Materials Used in Laminar Flow for Stem Cell Research
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Questions and Answers

What is the purpose of sterilizing the laminar flow unit with UV for half an hour before use?

  • To prevent contamination of the stem cells (correct)
  • To remove any residual chemicals
  • To prepare the unit for storage
  • To enhance laminar flow during the experiment

    • What is the purpose of using sterile petri dishes in stem cell isolation?

  • To store the isolated cells temporarily
  • To cultivate the isolated cells
  • To prepare the cells for enzymatic lysis
  • To collect and isolate the cells (correct)

    • What happens to the supernatant in the centrifuge during stem cell isolation?

  • It is used for further cell cultivation
  • It is discarded as waste (correct)
  • It is stored for future use
  • It is used to prepare the cells for enzymatic lysis

    • Why are the materials inside the laminar cabinet cleaned with 70% alcohol?

    <p>To sterilize the materials</p> Signup and view all the answers

    What is the purpose of physically dissecting the isolated tissues under a stereo microscope?

    <p>To remove any connective tissue</p> Signup and view all the answers

    What is the purpose of using automatic pipettes in stem cell isolation?

    <p>To handle the isolated cells carefully</p> Signup and view all the answers

    Which type of medium is specifically selected for the living condition and development of cells?

    <p>RPMI-16140 medium with L-Glutamine and sodium bicarbonate</p> Signup and view all the answers

    What is the primary purpose of the 0.22µm pore size filter system?

    <p>To purify the extra cells from waste materials</p> Signup and view all the answers

    What is the primary function of a +4°C refrigerator in a cell culture laboratory?

    <p>To store media and chemicals such as enzymes</p> Signup and view all the answers

    What is the primary purpose of a -20°C deep freezer in a cell culture laboratory?

    <p>To store frozen cells for a short time</p> Signup and view all the answers

    What is the purpose of Fetal Bovine Serum in a cell culture laboratory?

    <p>To supplement cell culture media</p> Signup and view all the answers

    What type of microscope is used to examine the morphology of living cells?

    <p>Inverted microscope</p> Signup and view all the answers

    What is the purpose of the laminar flow cabinet in cell culture?

    <p>To maintain a sterile environment for cell culture</p> Signup and view all the answers

    What is the function of the oven with carbon dioxide in cell culture?

    <p>To provide a controlled temperature and CO2 environment for cell growth</p> Signup and view all the answers

    What is the purpose of a centrifuge device in cell culture?

    <p>To separate cells from residual tissues</p> Signup and view all the answers

    What can be observed using an inverted microscope in cell culture?

    <p>All of the above</p> Signup and view all the answers

    What type of microscope is used to observe cells in 3D?

    <p>Stereomicroscope</p> Signup and view all the answers

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