Materials Used in Laminar Flow for Stem Cell Research
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Questions and Answers

What is the purpose of sterilizing the laminar flow unit with UV for half an hour before use?

  • To prevent contamination of the stem cells (correct)
  • To remove any residual chemicals
  • To prepare the unit for storage
  • To enhance laminar flow during the experiment

What is the purpose of using sterile petri dishes in stem cell isolation?

  • To store the isolated cells temporarily
  • To cultivate the isolated cells
  • To prepare the cells for enzymatic lysis
  • To collect and isolate the cells (correct)

What happens to the supernatant in the centrifuge during stem cell isolation?

  • It is used for further cell cultivation
  • It is discarded as waste (correct)
  • It is stored for future use
  • It is used to prepare the cells for enzymatic lysis

Why are the materials inside the laminar cabinet cleaned with 70% alcohol?

<p>To sterilize the materials (C)</p> Signup and view all the answers

What is the purpose of physically dissecting the isolated tissues under a stereo microscope?

<p>To remove any connective tissue (B)</p> Signup and view all the answers

What is the purpose of using automatic pipettes in stem cell isolation?

<p>To handle the isolated cells carefully (C)</p> Signup and view all the answers

Which type of medium is specifically selected for the living condition and development of cells?

<p>RPMI-16140 medium with L-Glutamine and sodium bicarbonate (B)</p> Signup and view all the answers

What is the primary purpose of the 0.22µm pore size filter system?

<p>To purify the extra cells from waste materials (A)</p> Signup and view all the answers

What is the primary function of a +4°C refrigerator in a cell culture laboratory?

<p>To store media and chemicals such as enzymes (B)</p> Signup and view all the answers

What is the primary purpose of a -20°C deep freezer in a cell culture laboratory?

<p>To store frozen cells for a short time (C)</p> Signup and view all the answers

What is the purpose of Fetal Bovine Serum in a cell culture laboratory?

<p>To supplement cell culture media (A)</p> Signup and view all the answers

What type of microscope is used to examine the morphology of living cells?

<p>Inverted microscope (D)</p> Signup and view all the answers

What is the purpose of the laminar flow cabinet in cell culture?

<p>To maintain a sterile environment for cell culture (C)</p> Signup and view all the answers

What is the function of the oven with carbon dioxide in cell culture?

<p>To provide a controlled temperature and CO2 environment for cell growth (B)</p> Signup and view all the answers

What is the purpose of a centrifuge device in cell culture?

<p>To separate cells from residual tissues (B)</p> Signup and view all the answers

What can be observed using an inverted microscope in cell culture?

<p>All of the above (D)</p> Signup and view all the answers

What type of microscope is used to observe cells in 3D?

<p>Stereomicroscope (A)</p> Signup and view all the answers

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