Materials Used in Laminar Flow for Stem Cell Research

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What is the purpose of sterilizing the laminar flow unit with UV for half an hour before use?

To prevent contamination of the stem cells (correct)

To remove any residual chemicals

To prepare the unit for storage

To enhance laminar flow during the experiment

What is the purpose of using sterile petri dishes in stem cell isolation?

To store the isolated cells temporarily

To cultivate the isolated cells

To prepare the cells for enzymatic lysis

To collect and isolate the cells (correct)

What happens to the supernatant in the centrifuge during stem cell isolation?

It is used for further cell cultivation

It is discarded as waste (correct)

It is stored for future use

It is used to prepare the cells for enzymatic lysis

Why are the materials inside the laminar cabinet cleaned with 70% alcohol?

To sterilize the materials Signup and view all the answers

What is the purpose of physically dissecting the isolated tissues under a stereo microscope?

To remove any connective tissue Signup and view all the answers

What is the purpose of using automatic pipettes in stem cell isolation?

To handle the isolated cells carefully Signup and view all the answers

Which type of medium is specifically selected for the living condition and development of cells?

RPMI-16140 medium with L-Glutamine and sodium bicarbonate Signup and view all the answers

What is the primary purpose of the 0.22µm pore size filter system?

To purify the extra cells from waste materials Signup and view all the answers

What is the primary function of a +4°C refrigerator in a cell culture laboratory?

To store media and chemicals such as enzymes Signup and view all the answers

What is the primary purpose of a -20°C deep freezer in a cell culture laboratory?

To store frozen cells for a short time Signup and view all the answers

What is the purpose of Fetal Bovine Serum in a cell culture laboratory?

To supplement cell culture media Signup and view all the answers

What type of microscope is used to examine the morphology of living cells?

Inverted microscope Signup and view all the answers

What is the purpose of the laminar flow cabinet in cell culture?

To maintain a sterile environment for cell culture Signup and view all the answers

What is the function of the oven with carbon dioxide in cell culture?

To provide a controlled temperature and CO2 environment for cell growth Signup and view all the answers

Materials Used in Laminar Flow for Stem Cell Research

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Questions and Answers

What is the purpose of sterilizing the laminar flow unit with UV for half an hour before use?

What is the purpose of using sterile petri dishes in stem cell isolation?

What happens to the supernatant in the centrifuge during stem cell isolation?

Why are the materials inside the laminar cabinet cleaned with 70% alcohol?

What is the purpose of physically dissecting the isolated tissues under a stereo microscope?

What is the purpose of using automatic pipettes in stem cell isolation?

Which type of medium is specifically selected for the living condition and development of cells?

What is the primary purpose of the 0.22µm pore size filter system?

What is the primary function of a +4°C refrigerator in a cell culture laboratory?

What is the primary purpose of a -20°C deep freezer in a cell culture laboratory?

What is the purpose of Fetal Bovine Serum in a cell culture laboratory?

What type of microscope is used to examine the morphology of living cells?

What is the purpose of the laminar flow cabinet in cell culture?

What is the function of the oven with carbon dioxide in cell culture?

What is the purpose of a centrifuge device in cell culture?

What can be observed using an inverted microscope in cell culture?

What type of microscope is used to observe cells in 3D?

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