Mastering Restriction Digestion and Directional Cloning for Expression Vectors

To see production of the new protein

To select for cells that have taken up the vector

To ligate vector and insert together

To ensure the insert is cloned in only one orientation (correct)

Digest vector and insert DNA with two restriction enzymes

Select for cells that have taken up vector by plating on selective media

Transform into E. coli (correct)

See production of new protein

To ensure the insert is cloned in only one orientation

To ligate vector and insert together

To select for cells that have taken up the vector (correct)

To see production of the new protein

<p>Directional cloning ensures the insert is cloned in only one orientation, while non-directional cloning does not</p> Signup and view all the answers

<p>To ensure the insert is cloned in only one orientation</p> Signup and view all the answers