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Questions and Answers
What is the purpose of restriction digestion in generating an expression vector?
What is the purpose of restriction digestion in generating an expression vector?
- To see production of the new protein
- To select for cells that have taken up the vector
- To ligate vector and insert together
- To ensure the insert is cloned in only one orientation (correct)
What is the next step after ligating vector and insert together in generating an expression vector?
What is the next step after ligating vector and insert together in generating an expression vector?
- Digest vector and insert DNA with two restriction enzymes
- Select for cells that have taken up vector by plating on selective media
- Transform into E. coli (correct)
- See production of new protein
What is the purpose of selective media in generating an expression vector?
What is the purpose of selective media in generating an expression vector?
- To ensure the insert is cloned in only one orientation
- To ligate vector and insert together
- To select for cells that have taken up the vector (correct)
- To see production of the new protein
What is the difference between directional cloning and non-directional cloning?
What is the difference between directional cloning and non-directional cloning?
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What is the purpose of digesting vector and insert DNA with two restriction enzymes in directional cloning?
What is the purpose of digesting vector and insert DNA with two restriction enzymes in directional cloning?
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