5 Questions
What is the importance of selecting a correct annealing temperature (At) for primer binding?
It increases the specificity of the PCR reaction
What is the formula for calculating the melting temperature (Tm) of a primer?
Tm = 2 (A + T) + 4 (C + G) degrees celsius
What is the relationship between annealing temperature (At) and melting temperature (Tm) of primers?
At should be 5 degrees celsius less than Tm
What factors determine the melting temperature (Tm) of a primer?
Both the length and base composition of the primer
What is the annealing temperature (At) for a primer with a melting temperature (Tm) of 58 degrees celsius?
53 degrees celsius
Test your knowledge of primer design with this quiz on annealing temperature (At) and melting temperature (Tm)! Learn about the importance of selecting the correct At for primer binding and how Tm is calculated based on base composition and primer size. Get ready to calculate Tm using the formula provided and improve your primer design skills.
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