Mastering Primer Design

It increases the specificity of the PCR reaction (correct)

It ensures that the primer will bind to the template DNA

It decreases the yield of the PCR reaction

It has no effect on the PCR reaction

Tm = 2 (C + G) + 4 (A + T) degrees celsius

Tm = 2 (A + T) + 4 (C + G) degrees celsius (correct)

Tm = 2 (A + C) + 4 (G + T) degrees celsius

Tm = 2 (G + T) + 4 (A + C) degrees celsius

At should be equal to Tm

At should be 5 degrees celsius less than Tm (correct)

At should be 5 degrees celsius more than Tm

At has no relationship with Tm

<p>Both the length and base composition of the primer</p> Signup and view all the answers

<p>53 degrees celsius</p> Signup and view all the answers