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Questions and Answers
What is the importance of selecting a correct annealing temperature (At) for primer binding?
What is the importance of selecting a correct annealing temperature (At) for primer binding?
- It increases the specificity of the PCR reaction (correct)
- It ensures that the primer will bind to the template DNA
- It decreases the yield of the PCR reaction
- It has no effect on the PCR reaction
What is the formula for calculating the melting temperature (Tm) of a primer?
What is the formula for calculating the melting temperature (Tm) of a primer?
- Tm = 2 (C + G) + 4 (A + T) degrees celsius
- Tm = 2 (A + T) + 4 (C + G) degrees celsius (correct)
- Tm = 2 (A + C) + 4 (G + T) degrees celsius
- Tm = 2 (G + T) + 4 (A + C) degrees celsius
What is the relationship between annealing temperature (At) and melting temperature (Tm) of primers?
What is the relationship between annealing temperature (At) and melting temperature (Tm) of primers?
- At should be equal to Tm
- At should be 5 degrees celsius less than Tm (correct)
- At should be 5 degrees celsius more than Tm
- At has no relationship with Tm
What factors determine the melting temperature (Tm) of a primer?
What factors determine the melting temperature (Tm) of a primer?
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What is the annealing temperature (At) for a primer with a melting temperature (Tm) of 58 degrees celsius?
What is the annealing temperature (At) for a primer with a melting temperature (Tm) of 58 degrees celsius?
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