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Questions and Answers
What is the importance of selecting a correct annealing temperature (At) for primer binding?
What is the formula for calculating the melting temperature (Tm) of a primer?
What is the relationship between annealing temperature (At) and melting temperature (Tm) of primers?
What factors determine the melting temperature (Tm) of a primer?
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What is the annealing temperature (At) for a primer with a melting temperature (Tm) of 58 degrees celsius?
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