Mastering Primer Design
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Questions and Answers

What is the importance of selecting a correct annealing temperature (At) for primer binding?

  • It increases the specificity of the PCR reaction (correct)
  • It ensures that the primer will bind to the template DNA
  • It decreases the yield of the PCR reaction
  • It has no effect on the PCR reaction

    • What is the formula for calculating the melting temperature (Tm) of a primer?

  • Tm = 2 (C + G) + 4 (A + T) degrees celsius
  • Tm = 2 (A + T) + 4 (C + G) degrees celsius (correct)
  • Tm = 2 (A + C) + 4 (G + T) degrees celsius
  • Tm = 2 (G + T) + 4 (A + C) degrees celsius

    • What is the relationship between annealing temperature (At) and melting temperature (Tm) of primers?

  • At should be equal to Tm
  • At should be 5 degrees celsius less than Tm (correct)
  • At should be 5 degrees celsius more than Tm
  • At has no relationship with Tm

    • What factors determine the melting temperature (Tm) of a primer?

    <p>Both the length and base composition of the primer</p> Signup and view all the answers

    What is the annealing temperature (At) for a primer with a melting temperature (Tm) of 58 degrees celsius?

    <p>53 degrees celsius</p> Signup and view all the answers

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