WEEK 7

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Which type of test is used to detect enzymatic or metabolic activity in bacteria based on their ability to utilize specific substrates?

Gram staining

Phenotypic identification methods

Enzyme-based tests (correct)

Antimicrobial susceptibility testing

What is the purpose of using antimicrobial agents in the testing process?

To enhance enzymatic reactions

To verify the accuracy of the test results (correct)

To promote bacterial growth

To inhibit bacterial growth

Which environmental factor is not necessary for the growth of obligate aerobes?

Carbon dioxide (correct)

Nutritional requirements

Oxygen

Temperature

Which type of test is used to determine the presence of catalase in bacteria?

Catalase test Signup and view all the answers

Which of the following is a component of duplex/hybrid in Northern Blot?

Guanine Signup and view all the answers

Which technique allows the synthesis of limitless quantities of target nucleic acid sequence?

Polymerase Chain Reaction Signup and view all the answers

Which technique separates double-stranded DNA in a PCR cycle?

Denaturation Signup and view all the answers

Which of the following best describes the purpose of the identification scheme in bacterial analysis?

To guide physician care of the patient Signup and view all the answers

What is the main purpose of determining the antimicrobial susceptibility profiles of a bacterial species?

To determine the appropriate antimicrobial therapy Signup and view all the answers

What is the primary purpose of collecting epidemiologic data in bacterial analysis?

To monitor the control and transmission of organisms Signup and view all the answers

Which of the following is a molecular diagnostic technique used in microbiology laboratory testing?

Amplification Signup and view all the answers

Which test is used to determine the capability of bacteria to oxidize or ferment sugar or carbohydrate?

Oxidative Fermentative (OF) test Signup and view all the answers

What is the purpose of nucleic acid hybridization in microbiology laboratory testing?

To detect the presence of an organism Signup and view all the answers

What is the purpose of adding mineral oil in one tube during the Oxidative Fermentative (OF) test?

To drive off oxygen Signup and view all the answers

What is the result if a bacteria is able to utilize glucose in the presence of oxygen (aerobic) but not in the absence of oxygen (anaerobic)?

Oxidative/Nonfermenter Signup and view all the answers

What is the purpose of the Phenylalanine deaminase test in microbiology?

To detect metabolic activity Signup and view all the answers

What is the purpose of the inhibitor profile test?

To detect the presence of antibiotics Signup and view all the answers

Which of the following is true about generation time in bacteria?

Generation time is the time it takes for a bacterium to reproduce into 2 bacteria. Signup and view all the answers

What is the time required for most conventional identification schemes?

18 to 24 hours Signup and view all the answers

Which of the following is NOT true about growth-based tests?

They are dependent on the generation time of bacteria. Signup and view all the answers

What is the purpose of primary media in specimen processing?

To check for the presence of colonies Signup and view all the answers

Which of the following is a characteristic of Real-Time PCR (RT-PCR)?

Amplicons are detected after each cycle Signup and view all the answers

What is the purpose of the thermal cycler in PCR?

To maintain a constant temperature during the PCR cycle Signup and view all the answers

Which component of PCR is responsible for starting the synthesis of new DNA strands?

Oligonucleotide Primers Signup and view all the answers

What is the function of Magnesium Chloride (MgCl2) in PCR?

To act as a cofactor for Taq polymerase Signup and view all the answers

What is the purpose of Deoxynucleotides in PCR?

To provide the building blocks for DNA synthesis Signup and view all the answers

What is the purpose of Nucleic Acid Sequencing?

To determine the exact nucleotide sequence of a gene Signup and view all the answers