27 Questions
Which type of test is used to detect enzymatic or metabolic activity in bacteria based on their ability to utilize specific substrates?
Enzyme-based tests
What is the purpose of using antimicrobial agents in the testing process?
To verify the accuracy of the test results
Which environmental factor is not necessary for the growth of obligate aerobes?
Carbon dioxide
Which type of test is used to determine the presence of catalase in bacteria?
Catalase test
Which of the following is a component of duplex/hybrid in Northern Blot?
Guanine
Which technique allows the synthesis of limitless quantities of target nucleic acid sequence?
Polymerase Chain Reaction
Which technique separates double-stranded DNA in a PCR cycle?
Denaturation
Which of the following best describes the purpose of the identification scheme in bacterial analysis?
To guide physician care of the patient
What is the main purpose of determining the antimicrobial susceptibility profiles of a bacterial species?
To determine the appropriate antimicrobial therapy
What is the primary purpose of collecting epidemiologic data in bacterial analysis?
To monitor the control and transmission of organisms
Which of the following is a molecular diagnostic technique used in microbiology laboratory testing?
Amplification
Which test is used to determine the capability of bacteria to oxidize or ferment sugar or carbohydrate?
Oxidative Fermentative (OF) test
What is the purpose of nucleic acid hybridization in microbiology laboratory testing?
To detect the presence of an organism
What is the purpose of adding mineral oil in one tube during the Oxidative Fermentative (OF) test?
To drive off oxygen
What is the result if a bacteria is able to utilize glucose in the presence of oxygen (aerobic) but not in the absence of oxygen (anaerobic)?
Oxidative/Nonfermenter
What is the purpose of the Phenylalanine deaminase test in microbiology?
To detect metabolic activity
What is the purpose of the inhibitor profile test?
To detect the presence of antibiotics
Which of the following is true about generation time in bacteria?
Generation time is the time it takes for a bacterium to reproduce into 2 bacteria.
What is the time required for most conventional identification schemes?
18 to 24 hours
Which of the following is NOT true about growth-based tests?
They are dependent on the generation time of bacteria.
What is the purpose of primary media in specimen processing?
To check for the presence of colonies
Which of the following is a characteristic of Real-Time PCR (RT-PCR)?
Amplicons are detected after each cycle
What is the purpose of the thermal cycler in PCR?
To maintain a constant temperature during the PCR cycle
Which component of PCR is responsible for starting the synthesis of new DNA strands?
Oligonucleotide Primers
What is the function of Magnesium Chloride (MgCl2) in PCR?
To act as a cofactor for Taq polymerase
What is the purpose of Deoxynucleotides in PCR?
To provide the building blocks for DNA synthesis
What is the purpose of Nucleic Acid Sequencing?
To determine the exact nucleotide sequence of a gene
Test your knowledge on Northern Blot, a technique that uses gel electrophoresis and solid support hybridization. Learn about the components of duplex/hybrid and the hydrogen bonds formed between base sequences. Challenge yourself to understand the role of hybridization in forming duplexes.
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