PCOL 5-1 Molecular techniques in neuropharmacology
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PCOL 5-1 Molecular techniques in neuropharmacology

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Questions and Answers

What is the purpose of a selectable marker in a plasmid/vector used for protein expression?

  • To turn expression of the protein on/off
  • To induce expression of the desired protein
  • To separate the desired protein from other proteins
  • To allow selection of desired bacteria only (correct)

    • What is the role of a C-terminal tag in protein expression?

  • To turn expression of the protein on/off
  • To separate the desired protein from other proteins
  • To induce expression of the protein
  • To allow separation of the desired protein once expressed (correct)

    • Which technique uses protein structure as the basis for designing new ligands?

  • Genetic template
  • Molecular modelling (correct)
  • Recombinant protein expression
  • Structural biology

    • What is the main purpose of recombinant protein expression?

    <p>To generate large amounts of protein of interest</p> Signup and view all the answers

    Why are living cells used in the process of recombinant protein expression?

    <p>Because proteins are too large and complex for easy chemical synthesis</p> Signup and view all the answers

    What is the primary focus of molecular modelling?

    <p>To design new ligands based on protein structure</p> Signup and view all the answers

    Which organism has the highest speed in protein expression?

    <p>Bacteria</p> Signup and view all the answers

    Which organism has the lowest cost in protein expression?

    <p>Bacteria</p> Signup and view all the answers

    Which organism has the highest yield in protein expression?

    <p>Yeast</p> Signup and view all the answers

    What is the purpose of gel filtration in protein purification?

    <p>To filter larger proteins more slowly</p> Signup and view all the answers

    What is required for functional analysis of proteins?

    <p>Proteins that are pure, stable, soluble, and plentiful</p> Signup and view all the answers

    What is the basis for fluorescence-based binding assays?

    <p>Conformational changes induced by drug binding</p> Signup and view all the answers

    What happens to untagged proteins in column-based protein purification?

    <p>They wash straight through</p> Signup and view all the answers

    What is the purpose of the C-terminal tag in protein expression?

    <p>To conjugate with Ni</p> Signup and view all the answers

    Which technique is used to measure thermodynamic parameters in solution between a ligand and a protein?

    <p>ITC Binding Assays</p> Signup and view all the answers

    What does drug binding in ITC Binding Assays always result in?

    <p>Exothermic reaction</p> Signup and view all the answers

    What is the primary purpose of Protein Crystallography?

    <p>To visualize proteins at high resolution (atomic scale)</p> Signup and view all the answers

    Which technique requires a synchrotron and the scattering of radiation to obtain a diffraction pattern?

    <p>Protein Crystallography</p> Signup and view all the answers

    What is the process of controlled evaporation used for in Crystallisation?

    <p>To form crystals</p> Signup and view all the answers

    Which type of proteins are relatively easy to crystallize?

    <p>Hydrophilic (soluble) proteins</p> Signup and view all the answers

    What is the primary use of Cryo-EM?

    <p>To investigate the detailed structure of tissues</p> Signup and view all the answers

    What percentage of encoded proteins are membrane-bound proteins?

    <p>30%</p> Signup and view all the answers

    What is the main reason why membrane-bound proteins are difficult to work with?

    <p>They are unstable and insoluble when removed from the membrane</p> Signup and view all the answers

    What percentage of drugs target membrane-bound proteins?

    <p>50%</p> Signup and view all the answers

    Which technique is used to visualize proteins at high resolution and understand conformational changes?

    <p>Protein Crystallography</p> Signup and view all the answers

    What is the resolution limit for X-ray crystallography?

    <p>1 A</p> Signup and view all the answers

    What is the primary purpose of Crystallisation in protein research?

    <p>To obtain an ordered, repeating pattern</p> Signup and view all the answers

    Why are membrane-bound proteins difficult to work with?

    <p>They are unstable and insoluble when removed from the membrane</p> Signup and view all the answers

    What is the main use of Cryo-EM?

    <p>To investigate the detailed structure of tissues, cells, organelles, and macromolecular complexes</p> Signup and view all the answers

    Study Notes

    Structure-Based Drug Design

    • Utilizes protein structure as a basis for designing new ligands based on principles of molecular recognition

    Molecular Modelling

    • Involves the generation, manipulation, or representation of 3D structures of molecules and their associated physico-chemical properties
    • Employs various computerized techniques based on theoretical chemistry and experimental data

    Structural Biology

    • Studies the molecular structure and dynamics of biological macromolecules
    • Examines how altered structure affects function
    • Incorporates molecular biology, biochemistry, and biophysics

    Recombinant Protein Expression

    • Requires large amounts of protein of interest
    • Uses living cells instead of chemical synthesis due to the large and complex nature of proteins
    • Needs a genetic template to produce the protein of interest

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    Description

    Test your knowledge on molecular techniques in neuropharmacology and structure-based drug design with this quiz. Explore the use of protein structure in designing new ligands, learn about molecular modelling techniques, and delve into the principles of molecular recognition.

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