29 Questions
What is the purpose of a selectable marker in a plasmid/vector used for protein expression?
To allow selection of desired bacteria only
What is the role of a C-terminal tag in protein expression?
To allow separation of the desired protein once expressed
Which technique uses protein structure as the basis for designing new ligands?
Molecular modelling
What is the main purpose of recombinant protein expression?
To generate large amounts of protein of interest
Why are living cells used in the process of recombinant protein expression?
Because proteins are too large and complex for easy chemical synthesis
What is the primary focus of molecular modelling?
To design new ligands based on protein structure
Which organism has the highest speed in protein expression?
Bacteria
Which organism has the lowest cost in protein expression?
Bacteria
Which organism has the highest yield in protein expression?
Yeast
What is the purpose of gel filtration in protein purification?
To filter larger proteins more slowly
What is required for functional analysis of proteins?
Proteins that are pure, stable, soluble, and plentiful
What is the basis for fluorescence-based binding assays?
Conformational changes induced by drug binding
What happens to untagged proteins in column-based protein purification?
They wash straight through
What is the purpose of the C-terminal tag in protein expression?
To conjugate with Ni
Which technique is used to measure thermodynamic parameters in solution between a ligand and a protein?
ITC Binding Assays
What does drug binding in ITC Binding Assays always result in?
Exothermic reaction
What is the primary purpose of Protein Crystallography?
To visualize proteins at high resolution (atomic scale)
Which technique requires a synchrotron and the scattering of radiation to obtain a diffraction pattern?
Protein Crystallography
What is the process of controlled evaporation used for in Crystallisation?
To form crystals
Which type of proteins are relatively easy to crystallize?
Hydrophilic (soluble) proteins
What is the primary use of Cryo-EM?
To investigate the detailed structure of tissues
What percentage of encoded proteins are membrane-bound proteins?
30%
What is the main reason why membrane-bound proteins are difficult to work with?
They are unstable and insoluble when removed from the membrane
What percentage of drugs target membrane-bound proteins?
50%
Which technique is used to visualize proteins at high resolution and understand conformational changes?
Protein Crystallography
What is the resolution limit for X-ray crystallography?
1 A
What is the primary purpose of Crystallisation in protein research?
To obtain an ordered, repeating pattern
Why are membrane-bound proteins difficult to work with?
They are unstable and insoluble when removed from the membrane
What is the main use of Cryo-EM?
To investigate the detailed structure of tissues, cells, organelles, and macromolecular complexes
Study Notes
Structure-Based Drug Design
- Utilizes protein structure as a basis for designing new ligands based on principles of molecular recognition
Molecular Modelling
- Involves the generation, manipulation, or representation of 3D structures of molecules and their associated physico-chemical properties
- Employs various computerized techniques based on theoretical chemistry and experimental data
Structural Biology
- Studies the molecular structure and dynamics of biological macromolecules
- Examines how altered structure affects function
- Incorporates molecular biology, biochemistry, and biophysics
Recombinant Protein Expression
- Requires large amounts of protein of interest
- Uses living cells instead of chemical synthesis due to the large and complex nature of proteins
- Needs a genetic template to produce the protein of interest
Test your knowledge on molecular techniques in neuropharmacology and structure-based drug design with this quiz. Explore the use of protein structure in designing new ligands, learn about molecular modelling techniques, and delve into the principles of molecular recognition.
Make Your Own Quizzes and Flashcards
Convert your notes into interactive study material.
Get started for free
