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Questions and Answers
What is the purpose of a selectable marker in a plasmid/vector used for protein expression?
What is the role of a C-terminal tag in protein expression?
Which technique uses protein structure as the basis for designing new ligands?
What is the main purpose of recombinant protein expression?
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Why are living cells used in the process of recombinant protein expression?
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What is the primary focus of molecular modelling?
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Which organism has the highest speed in protein expression?
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Which organism has the lowest cost in protein expression?
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Which organism has the highest yield in protein expression?
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What is the purpose of gel filtration in protein purification?
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What is required for functional analysis of proteins?
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What is the basis for fluorescence-based binding assays?
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What happens to untagged proteins in column-based protein purification?
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What is the purpose of the C-terminal tag in protein expression?
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Which technique is used to measure thermodynamic parameters in solution between a ligand and a protein?
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What does drug binding in ITC Binding Assays always result in?
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What is the primary purpose of Protein Crystallography?
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Which technique requires a synchrotron and the scattering of radiation to obtain a diffraction pattern?
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What is the process of controlled evaporation used for in Crystallisation?
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Which type of proteins are relatively easy to crystallize?
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What is the primary use of Cryo-EM?
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What percentage of encoded proteins are membrane-bound proteins?
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What is the main reason why membrane-bound proteins are difficult to work with?
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What percentage of drugs target membrane-bound proteins?
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Which technique is used to visualize proteins at high resolution and understand conformational changes?
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What is the resolution limit for X-ray crystallography?
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What is the primary purpose of Crystallisation in protein research?
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Why are membrane-bound proteins difficult to work with?
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What is the main use of Cryo-EM?
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Study Notes
Structure-Based Drug Design
- Utilizes protein structure as a basis for designing new ligands based on principles of molecular recognition
Molecular Modelling
- Involves the generation, manipulation, or representation of 3D structures of molecules and their associated physico-chemical properties
- Employs various computerized techniques based on theoretical chemistry and experimental data
Structural Biology
- Studies the molecular structure and dynamics of biological macromolecules
- Examines how altered structure affects function
- Incorporates molecular biology, biochemistry, and biophysics
Recombinant Protein Expression
- Requires large amounts of protein of interest
- Uses living cells instead of chemical synthesis due to the large and complex nature of proteins
- Needs a genetic template to produce the protein of interest
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Description
Test your knowledge on molecular techniques in neuropharmacology and structure-based drug design with this quiz. Explore the use of protein structure in designing new ligands, learn about molecular modelling techniques, and delve into the principles of molecular recognition.