Podcast
Questions and Answers
What is the purpose of a selectable marker in a plasmid/vector used for protein expression?
What is the purpose of a selectable marker in a plasmid/vector used for protein expression?
What is the role of a C-terminal tag in protein expression?
What is the role of a C-terminal tag in protein expression?
Which technique uses protein structure as the basis for designing new ligands?
Which technique uses protein structure as the basis for designing new ligands?
What is the main purpose of recombinant protein expression?
What is the main purpose of recombinant protein expression?
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Why are living cells used in the process of recombinant protein expression?
Why are living cells used in the process of recombinant protein expression?
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What is the primary focus of molecular modelling?
What is the primary focus of molecular modelling?
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Which organism has the highest speed in protein expression?
Which organism has the highest speed in protein expression?
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Which organism has the lowest cost in protein expression?
Which organism has the lowest cost in protein expression?
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Which organism has the highest yield in protein expression?
Which organism has the highest yield in protein expression?
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What is the purpose of gel filtration in protein purification?
What is the purpose of gel filtration in protein purification?
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What is required for functional analysis of proteins?
What is required for functional analysis of proteins?
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What is the basis for fluorescence-based binding assays?
What is the basis for fluorescence-based binding assays?
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What happens to untagged proteins in column-based protein purification?
What happens to untagged proteins in column-based protein purification?
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What is the purpose of the C-terminal tag in protein expression?
What is the purpose of the C-terminal tag in protein expression?
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Which technique is used to measure thermodynamic parameters in solution between a ligand and a protein?
Which technique is used to measure thermodynamic parameters in solution between a ligand and a protein?
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What does drug binding in ITC Binding Assays always result in?
What does drug binding in ITC Binding Assays always result in?
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What is the primary purpose of Protein Crystallography?
What is the primary purpose of Protein Crystallography?
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Which technique requires a synchrotron and the scattering of radiation to obtain a diffraction pattern?
Which technique requires a synchrotron and the scattering of radiation to obtain a diffraction pattern?
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What is the process of controlled evaporation used for in Crystallisation?
What is the process of controlled evaporation used for in Crystallisation?
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Which type of proteins are relatively easy to crystallize?
Which type of proteins are relatively easy to crystallize?
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What is the primary use of Cryo-EM?
What is the primary use of Cryo-EM?
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What percentage of encoded proteins are membrane-bound proteins?
What percentage of encoded proteins are membrane-bound proteins?
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What is the main reason why membrane-bound proteins are difficult to work with?
What is the main reason why membrane-bound proteins are difficult to work with?
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What percentage of drugs target membrane-bound proteins?
What percentage of drugs target membrane-bound proteins?
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Which technique is used to visualize proteins at high resolution and understand conformational changes?
Which technique is used to visualize proteins at high resolution and understand conformational changes?
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What is the resolution limit for X-ray crystallography?
What is the resolution limit for X-ray crystallography?
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What is the primary purpose of Crystallisation in protein research?
What is the primary purpose of Crystallisation in protein research?
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Why are membrane-bound proteins difficult to work with?
Why are membrane-bound proteins difficult to work with?
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What is the main use of Cryo-EM?
What is the main use of Cryo-EM?
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Study Notes
Structure-Based Drug Design
- Utilizes protein structure as a basis for designing new ligands based on principles of molecular recognition
Molecular Modelling
- Involves the generation, manipulation, or representation of 3D structures of molecules and their associated physico-chemical properties
- Employs various computerized techniques based on theoretical chemistry and experimental data
Structural Biology
- Studies the molecular structure and dynamics of biological macromolecules
- Examines how altered structure affects function
- Incorporates molecular biology, biochemistry, and biophysics
Recombinant Protein Expression
- Requires large amounts of protein of interest
- Uses living cells instead of chemical synthesis due to the large and complex nature of proteins
- Needs a genetic template to produce the protein of interest
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Description
Test your knowledge on molecular techniques in neuropharmacology and structure-based drug design with this quiz. Explore the use of protein structure in designing new ligands, learn about molecular modelling techniques, and delve into the principles of molecular recognition.
