5 Questions
What is the purpose of fixation in light microscopy?
The purpose of fixation in light microscopy is to retard the alterations of tissue subsequent to death and maintain its normal architecture.
What are the objectives of fixation?
The objectives of fixation are to prevent autolysis and bacterial attack, to fix the tissues so that their volume and shape will not change during processing, to prepare the tissue for clear staining of sections, and to leave the tissue as close as possible to its living state without losing small molecules.
What is the reaction that occurs during fixation?
Fixation occurs by a reaction between the fixative and protein, which forms a gel and keeps everything in their in vivo relation to each other.
List the steps involved in the processing of the tissue for light microscopy.
The steps involved in the processing of the tissue for light microscopy are fixation, dehydration in a series of acetone, embedding in paraffin wax, sectioning, and staining with Hematoxylin-Eosin (H&E).
What is the thickness of the sections used in light microscopy?
The sections used in light microscopy are 4-5µ thick.
This quiz covers the process of light microscopy, specifically focusing on fixation, processing, sectioning, embedding, and staining of samples. Learn about the steps involved and how to examine samples under a light microscope.
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