Electron Microscopy Tissue Fixation Quiz

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Which step in the fixation process is considered the most critical?

Decalcification

Infiltration

Trimming

Dehydration (correct)

What is the primary purpose of fixation in tissue preparation?

To harden and protect the tissue

To prevent degeneration and decomposition

To make cutting easier

To preserve the morphological and chemical integrity of the cell (correct)

Which process follows fixation in tissue preparation?

Dehydration (correct)

Trimming

Decalcification

Infiltration

What happens if fixation is not adequate in tissue preparation?

The tissue will become brittle during dehydration Signup and view all the answers

Which fixative is recommended for fixation of surgical specimens at room temperature?

10% formaldehyde Signup and view all the answers

Which fixative is composed of mercuric chloride and glacial acetic acid?

Zenker's solution Signup and view all the answers

Which fixative is suitable for cytoplasmic fixation?

Flemming's fluid without acetic acid Signup and view all the answers

Which fixative is recommended for histochemical studies?

10% formol saline Signup and view all the answers

Which fixative is recommended for the preservation and storage of surgical, post-mortem, and research specimens?

Formal Saline Signup and view all the answers

Which fixative is the best for preserving iron pigments and elastic fibers?

Unbuffered Zinc Formalin Signup and view all the answers

Which fixative is recommended for routine post-mortem tissues?

Formol Corrosive Signup and view all the answers

Which fixative is commonly used for electron microscopy?

Glutaraldehyde Signup and view all the answers

Which fixative is recommended for fixing adrenal glands in vapor form?

Osmium tetroxide Signup and view all the answers

Which fixative completely permeabilizes cell membranes?

Osmium tetroxide Signup and view all the answers

Which fixative is pale yellow in color and can cause blindness with direct contact?

Picric acid Signup and view all the answers

Which fixative is not recommended as a morphological fixative for tissue blocks due to shrinkage and poor preservation effects?

Acetone Signup and view all the answers

Which fixative is recommended for connective tissue samples in trichrome staining?

Zenker-Formol (Helly's) Solution Signup and view all the answers

Which fixative is an excellent micro anatomic fixative for pituitary, BM, spleen, and liver, but can produce brown pigment if fixed for more than 24 hours?

Zenker-Formol (Helly's) Solution Signup and view all the answers

Which fixative is recommended for tumor biopsies (skin) and is composed of mercuric chloride, sodium trichloroacetic acid, and formaldehyde?

Heidenhain's Susa Signup and view all the answers

Which fixative is recommended for the use of BM biopsy, specifically for normal and abnormal cell types of bone marrow?

Lillie's B-5 Fixative Signup and view all the answers

Which fixative is commonly used for fixing blood, tissue films, and smears, but cannot be mixed with chromic acid or potassium dichromate?

Ethyl alcohol Signup and view all the answers

Which fixative is the most rapid fixative, commonly used for fixing chromosomes and brain tissues for the diagnosis of rabies?

Carnoy's fixative Signup and view all the answers

Which fixative can be used for rapid diagnosis, fixes and dehydrates at the same time in the frozen section room, and is used to fix sputum?

Gendre's fixative Signup and view all the answers

Which fixative is recommended for fixing mucopolysaccharides and nuclear proteins, and can produce fair results after conventional processing if fixation time is kept very short?

Newcomer's fluid Signup and view all the answers

Which of the following is NOT a method used for washing out excess fixative?

Alcoholic iodine Signup and view all the answers

What is the purpose of decalcification?

To make tissues flexible and easier to cut Signup and view all the answers

Which decalcifying agent should be changed regularly during the decalcification process?

EDTA (Versene) Signup and view all the answers

What is the purpose of secondary fixation?

To remove excess fixative from the tissue Signup and view all the answers

Which of the following is NOT a factor that affects fixation?

Electrical ionization Signup and view all the answers

What is the disadvantage of heat fixation using a microwave?

It is only suitable for small tissues Signup and view all the answers