Mastering ISSR Amplification

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AmbitiousConnemara
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What is the size range of ISSRs?

100-3000 bp

Which technique uses microsatellite core sequences as primers with selective nucleotides as anchors into non-repeat adjacent regions?

ISSR analysis

How are ISSRs separated for analysis?

Gel electrophoresis

Which technique uses a single primer containing only the core motif of a minisatellite?

DAMD

What is the purpose of scoring ISSRs as the presence or absence of fragments of particular size?

To measure the size of the DNA fragments

Test your knowledge on ISSRs and their amplification using PCR in this quiz. Learn about the selective nucleotides used as anchors and the process of scoring the generated fragments through gel electrophoresis.

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