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Questions and Answers
Which method of ISSR markers is ranked as the most technically demanding and costly?
Which method of ISSR markers is ranked as the most technically demanding and costly?
- Manual agarose
- Capillary electrophoresis
- Automated using fluorescently labeled primers (correct)
- Manual polyacrylamide with silver staining
What are the advantages of using ISSR markers?
What are the advantages of using ISSR markers?
- They have limited applications
- They can only be used in specific plant species
- They are expensive compared to other methods
- They are easy to develop (correct)
Which method of ISSR markers is considered a low-cost method for inferring plant genetic diversity?
Which method of ISSR markers is considered a low-cost method for inferring plant genetic diversity?
- Automated using fluorescently labeled primers
- Capillary electrophoresis
- Manual agarose (correct)
- Manual polyacrylamide with silver staining
What is the flexibility of the basic technique of ISSRs?
What is the flexibility of the basic technique of ISSRs?
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What is the potential universality of ISSR primers?
What is the potential universality of ISSR primers?
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Study Notes
ISSR Markers
- Radiolabeling method of ISSR markers is ranked as the most technically demanding and costly.
- Advantages of using ISSR markers include:
- High reproductive ability
- High variability in the DNA sequence
- Ability to detect polymorphisms
- Ability to detect genetic diversity
- The PCR-based method of ISSR markers is considered a low-cost method for inferring plant genetic diversity.
- The basic technique of ISSRs is flexible, allowing for the use of different primers and PCR conditions to optimize the results.
- ISSR primers have potential universality, meaning they can be used across different species and genera.
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