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Which fixative is recommended for CNS and postmortem tissues for histochemical examination?
Which fixative is best for iron pigments and elastic fibers?
Which fixative is suitable for use when preparing samples for light microscopy in resin embedding and sectioning, and for electron microscopy?
Which fixative is made up of 2 formaldehyde residues linked by 3 carbon chains?
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Which fixative is recommended for routine mortem tissues?
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Which fixative is an alternative to mercuric chloride formulations and provides improved results with immunohistochemistry?
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Which fixative is the most rapid fixative and is used for the diagnosis of rabies?
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Which fixative is commonly used for routine paraffin sections?
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What is the recommended concentration for formaldehyde fixative?
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Which fixative is the best general tissue fixative?
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What is the pH of 10% Formol Saline?
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Which fixative is recommended for paraffin embedding, immunohistochemistry, and fluorescent in-situ hybridization (FSH)?
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What is the purpose of adding magnesium carbonate or CaCO3 to formaldehyde fixative?
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How often should the formalin/formaldehyde be changed if it is old?
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Which of the following is a potential effect of exposure to acid vapor?
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What is the fixation time for acid vapor?
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Which of the following is true about the preservation effects of acid vapor?
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At what temperature should acid vapor be used for fixation of cryostat sections?
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What is the recommended fixation time for small tissue blocks using acid vapor?
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What is the potential effect of acid vapor on tissue blocks?
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What type of sections can be fixed with acid vapor in several minutes?
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Which of the following is a potential effect of exposure to acid vapor on the cornea?
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What is the effect of acid vapor on hematoxylin?
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Which fixative is recommended for renal tissue, fibrin, connective tissues, and muscles?
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Which fixative is recommended for liver, spleen, CT fibers, and nuclei?
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Which fixative is recommended for trichrome staining of connective tissue?
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Which fixative is recommended for tumor biopsies (skin)?
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Which fixative is recommended for BM biopsy?
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Which fixative is recommended for fixation of embryos and pituitary biopsies?
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Which fixative is recommended for GIT specimens and endocrine tissues?
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Study Notes
Fixatives for Histochemical Examination
- Bouin's fixative is recommended for CNS and postmortem tissues.
- Hollande's fixative is best for iron pigments and elastic fibers.
- Glutaraldehyde is suitable for preparing samples for light microscopy in resin embedding and sectioning, and for electron microscopy.
- Formaldehyde is made up of 2 formaldehyde residues linked by 3 carbon chains.
- Formaldehyde is recommended for routine mortem tissues.
- Zinc formalin is an alternative to mercuric chloride formulations and provides improved results with immunohistochemistry.
- Bouin's fixative is the most rapid fixative and is used for the diagnosis of rabies.
- Formaldehyde is commonly used for routine paraffin sections.
- The recommended concentration for formaldehyde fixative is 10%.
- Formaldehyde is the best general tissue fixative.
- The pH of 10% Formol Saline is 7.4.
Additional Fixative Information
- Adding magnesium carbonate or CaCO3 to formaldehyde fixative helps to neutralize acidic pH.
- Formalin/formaldehyde should be changed every 2-3 weeks if it is old.
- Exposure to acid vapor can cause corrosion to equipment and mucous membrane irritation.
- The fixation time for acid vapor is 15-30 minutes.
- Acid vapor helps to preserve morphology and enzymatic activity.
- Acid vapor should be used at 4-8°C for fixation of cryostat sections.
- The recommended fixation time for small tissue blocks using acid vapor is 15-30 minutes.
- Acid vapor can cause tissue blocks to become brittle.
- Cryostat sections can be fixed with acid vapor in several minutes.
- Exposure to acid vapor can cause corneal damage.
- Acid vapor can cause hematoxylin to precipitate.
Tissue-Specific Fixatives
- Zenker's fixative is recommended for renal tissue, fibrin, connective tissues, and muscles.
- Formaldehyde is recommended for liver, spleen, CT fibers, and nuclei.
- Bouin's fixative is recommended for trichrome staining of connective tissue.
- Formaldehyde is recommended for tumor biopsies (skin).
- Zenker's fixative is recommended for BM biopsy.
- Bouin's fixative is recommended for fixation of embryos and pituitary biopsies.
- Formaldehyde is recommended for GIT specimens and endocrine tissues.
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Description
Test your knowledge on fixatives used in tissue processing with this quiz! Learn about the characteristics, precautions, advantages, and disadvantages of popular fixatives like Mercuric Chloride Fixative, Zenker's Fluid, Zenker-Formol Solution, and more. Perfect for those interested in histology and tissue photography.
