HCT - FIXATIVE TISSUES - UNFINAL
30 Questions
4 Views

Choose a study mode

Play Quiz
Study Flashcards
Spaced Repetition
Chat to lesson

Podcast

Play an AI-generated podcast conversation about this lesson

Questions and Answers

Which fixative is recommended for CNS and postmortem tissues for histochemical examination?

  • Glutaraldehyde
  • Karnovsky's fixative (correct)
  • Formolcorrosive
  • Zinc formalin

    • Which fixative is best for iron pigments and elastic fibers?

  • Glutaraldehyde
  • Zinc formalin
  • Formolcorrosive (correct)
  • Karnovsky's fixative

    • Which fixative is suitable for use when preparing samples for light microscopy in resin embedding and sectioning, and for electron microscopy?

  • Glutaraldehyde
  • Formolcorrosive
  • Karnovsky's fixative
  • Zinc formalin (correct)

    • Which fixative is made up of 2 formaldehyde residues linked by 3 carbon chains?

    <p>Glutaraldehyde</p> Signup and view all the answers

    Which fixative is recommended for routine mortem tissues?

    <p>Formolcorrosive</p> Signup and view all the answers

    Which fixative is an alternative to mercuric chloride formulations and provides improved results with immunohistochemistry?

    <p>Zinc formalin</p> Signup and view all the answers

    Which fixative is the most rapid fixative and is used for the diagnosis of rabies?

    <p>Karnovsky's fixative</p> Signup and view all the answers

    Which fixative is commonly used for routine paraffin sections?

    <p>Neutral Buffered Formalin</p> Signup and view all the answers

    What is the recommended concentration for formaldehyde fixative?

    <p>10%</p> Signup and view all the answers

    Which fixative is the best general tissue fixative?

    <p>Neutral Buffered Formalin</p> Signup and view all the answers

    What is the pH of 10% Formol Saline?

    <p>7</p> Signup and view all the answers

    Which fixative is recommended for paraffin embedding, immunohistochemistry, and fluorescent in-situ hybridization (FSH)?

    <p>Neutral Buffered Formalin</p> Signup and view all the answers

    What is the purpose of adding magnesium carbonate or CaCO3 to formaldehyde fixative?

    <p>To remove acid formaldehyde hematin</p> Signup and view all the answers

    How often should the formalin/formaldehyde be changed if it is old?

    <p>Every 3 months</p> Signup and view all the answers

    Which of the following is a potential effect of exposure to acid vapor?

    <p>Irritation of eyes</p> Signup and view all the answers

    What is the fixation time for acid vapor?

    <p>24 - 48 hours</p> Signup and view all the answers

    Which of the following is true about the preservation effects of acid vapor?

    <p>It dissolves fat</p> Signup and view all the answers

    At what temperature should acid vapor be used for fixation of cryostat sections?

    <p>0°C to 4°C</p> Signup and view all the answers

    What is the recommended fixation time for small tissue blocks using acid vapor?

    <p>1 - 24 hours</p> Signup and view all the answers

    What is the potential effect of acid vapor on tissue blocks?

    <p>Shrinkage</p> Signup and view all the answers

    What type of sections can be fixed with acid vapor in several minutes?

    <p>Cell smears</p> Signup and view all the answers

    Which of the following is a potential effect of exposure to acid vapor on the cornea?

    <p>Blindness</p> Signup and view all the answers

    What is the effect of acid vapor on hematoxylin?

    <p>It inhibits hematoxylin</p> Signup and view all the answers

    Which fixative is recommended for renal tissue, fibrin, connective tissues, and muscles?

    <p>Mercuric chloride</p> Signup and view all the answers

    Which fixative is recommended for liver, spleen, CT fibers, and nuclei?

    <p>Mercuric chloride with glacial acetic acid</p> Signup and view all the answers

    Which fixative is recommended for trichrome staining of connective tissue?

    <p>Mercuric chloride with glacial acetic acid</p> Signup and view all the answers

    Which fixative is recommended for tumor biopsies (skin)?

    <p>Mercuric chloride with sodium chloride iodine</p> Signup and view all the answers

    Which fixative is recommended for BM biopsy?

    <p>Mercuric chloride with 4% formaldehyde and 0.22% acetic acid</p> Signup and view all the answers

    Which fixative is recommended for fixation of embryos and pituitary biopsies?

    <p>Brasil's alcoholic picroformol (w/ TCA)</p> Signup and view all the answers

    Which fixative is recommended for GIT specimens and endocrine tissues?

    <p>Hollande's fixative</p> Signup and view all the answers

    Study Notes

    Fixatives for Histochemical Examination

    • Bouin's fixative is recommended for CNS and postmortem tissues.
    • Hollande's fixative is best for iron pigments and elastic fibers.
    • Glutaraldehyde is suitable for preparing samples for light microscopy in resin embedding and sectioning, and for electron microscopy.
    • Formaldehyde is made up of 2 formaldehyde residues linked by 3 carbon chains.
    • Formaldehyde is recommended for routine mortem tissues.
    • Zinc formalin is an alternative to mercuric chloride formulations and provides improved results with immunohistochemistry.
    • Bouin's fixative is the most rapid fixative and is used for the diagnosis of rabies.
    • Formaldehyde is commonly used for routine paraffin sections.
    • The recommended concentration for formaldehyde fixative is 10%.
    • Formaldehyde is the best general tissue fixative.
    • The pH of 10% Formol Saline is 7.4.

    Additional Fixative Information

    • Adding magnesium carbonate or CaCO3 to formaldehyde fixative helps to neutralize acidic pH.
    • Formalin/formaldehyde should be changed every 2-3 weeks if it is old.
    • Exposure to acid vapor can cause corrosion to equipment and mucous membrane irritation.
    • The fixation time for acid vapor is 15-30 minutes.
    • Acid vapor helps to preserve morphology and enzymatic activity.
    • Acid vapor should be used at 4-8°C for fixation of cryostat sections.
    • The recommended fixation time for small tissue blocks using acid vapor is 15-30 minutes.
    • Acid vapor can cause tissue blocks to become brittle.
    • Cryostat sections can be fixed with acid vapor in several minutes.
    • Exposure to acid vapor can cause corneal damage.
    • Acid vapor can cause hematoxylin to precipitate.

    Tissue-Specific Fixatives

    • Zenker's fixative is recommended for renal tissue, fibrin, connective tissues, and muscles.
    • Formaldehyde is recommended for liver, spleen, CT fibers, and nuclei.
    • Bouin's fixative is recommended for trichrome staining of connective tissue.
    • Formaldehyde is recommended for tumor biopsies (skin).
    • Zenker's fixative is recommended for BM biopsy.
    • Bouin's fixative is recommended for fixation of embryos and pituitary biopsies.
    • Formaldehyde is recommended for GIT specimens and endocrine tissues.

    Studying That Suits You

    Use AI to generate personalized quizzes and flashcards to suit your learning preferences.

    Quiz Team

    Related Documents

    HCT-FIXATIVES-TABLE.pdf PDF

    Description

    Test your knowledge on fixatives used in tissue processing with this quiz! Learn about the characteristics, precautions, advantages, and disadvantages of popular fixatives like Mercuric Chloride Fixative, Zenker's Fluid, Zenker-Formol Solution, and more. Perfect for those interested in histology and tissue photography.

    More Like This

    Use Quizgecko on...
    Browser
    Open
    Browser
    Browser