Podcast
Questions and Answers
What is the primary function of Mercuric Chloride fixatives?
What is the primary function of Mercuric Chloride fixatives?
- Applied in Ultrathin Sections (correct)
- Used for fixing CNS tissues
- Mainly used in Enzyme Histochemistry
- Utilized for sputum samples
What are the disadvantages of formaldehyde fixatives mentioned in the text?
What are the disadvantages of formaldehyde fixatives mentioned in the text?
- Can cause allergic dermatitis (correct)
- Commonly applied in Ultrathin Sections
- May produce black granular deposits
- Used for Enzyme Histochemistry
When is a concentration of 4% formaldehyde usually utilized?
When is a concentration of 4% formaldehyde usually utilized?
- For fixing CNS tissues
- For fixing larger tissues (correct)
- For fixing sputum samples
- For Plastic Embedding
Which type of fixative is often used for Central Nervous System (CNS) tissues?
Which type of fixative is often used for Central Nervous System (CNS) tissues?
What is a common function of Chromate Fixatives?
What is a common function of Chromate Fixatives?
Which fixative should not contain Osmic acid?
Which fixative should not contain Osmic acid?
Which fixative is known for preserving the nucleus and chromatin material of the cell?
Which fixative is known for preserving the nucleus and chromatin material of the cell?
Which fixative renders the pH ≤4.6 and preserves membrane-bound organelles?
Which fixative renders the pH ≤4.6 and preserves membrane-bound organelles?
Which fixative should not contain glacial acetic acid?
Which fixative should not contain glacial acetic acid?
Which fixative is used to determine fat contents, presence of enzymes, and mucopolysaccharides?
Which fixative is used to determine fat contents, presence of enzymes, and mucopolysaccharides?
Which fixative is an aldehyde fixative?
Which fixative is an aldehyde fixative?
What is the most commonly utilized fixative in the laboratory?
What is the most commonly utilized fixative in the laboratory?
What is the purpose of using phosphate buffers in formalin fixation?
What is the purpose of using phosphate buffers in formalin fixation?
What component is used to dilute Formaldehyde to make a 10% solution for routine processing?
What component is used to dilute Formaldehyde to make a 10% solution for routine processing?
In which fixative do we see the use of Picric Acid and Glacial Acetic Acid together?
In which fixative do we see the use of Picric Acid and Glacial Acetic Acid together?
Which fixative is known as 'Formol sublimate' and contains Chloride?
Which fixative is known as 'Formol sublimate' and contains Chloride?
At what concentration is Glutaraldehyde often used for small tissues?
At what concentration is Glutaraldehyde often used for small tissues?
Study Notes
Fixatives
- Mercuric Chloride fixatives' primary function is to fix and preserve tissues.
- Formaldehyde fixatives have disadvantages, including being toxic and carcinogenic, and causing tissue shrinkage and hardening.
- A 4% concentration of formaldehyde is usually utilized for routine tissue fixation.
- Glutaraldehyde is often used for fixing Central Nervous System (CNS) tissues.
- Chromate Fixatives are used to preserve lipids and membranes.
- Osmic acid should not be used in Formaldehyde fixatives.
- Formaldehyde is known for preserving the nucleus and chromatin material of the cell.
- Acetic acid fixatives render the pH ≤4.6 and preserve membrane-bound organelles.
- Glacial acetic acid should not be used in Formaldehyde fixatives.
- Fat-containing fixatives, such as OsO4, are used to determine fat contents, presence of enzymes, and mucopolysaccharides.
- Formaldehyde is an aldehyde fixative.
- The most commonly utilized fixative in the laboratory is Formaldehyde.
- Phosphate buffers are used in formalin fixation to stabilize the pH and prevent tissue autolysis.
- Distilled water is used to dilute Formaldehyde to make a 10% solution for routine processing.
- Bouin's fixative uses Picric Acid and Glacial Acetic Acid together.
- Formol sublimate, which contains Chloride, is another name for Mercuric Chloride fixative.
- Glutaraldehyde is often used at a concentration of 2-3% for small tissues.
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Description
Test your knowledge on fixatives used in histopathology, including cytocological and histochemical fixatives. Learn about the different types of fixatives and their specific actions and remarks.
