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Questions and Answers
What is the primary function of Mercuric Chloride fixatives?
What are the disadvantages of formaldehyde fixatives mentioned in the text?
When is a concentration of 4% formaldehyde usually utilized?
Which type of fixative is often used for Central Nervous System (CNS) tissues?
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What is a common function of Chromate Fixatives?
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Which fixative should not contain Osmic acid?
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Which fixative is known for preserving the nucleus and chromatin material of the cell?
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Which fixative renders the pH ≤4.6 and preserves membrane-bound organelles?
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Which fixative should not contain glacial acetic acid?
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Which fixative is used to determine fat contents, presence of enzymes, and mucopolysaccharides?
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Which fixative is an aldehyde fixative?
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What is the most commonly utilized fixative in the laboratory?
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What is the purpose of using phosphate buffers in formalin fixation?
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What component is used to dilute Formaldehyde to make a 10% solution for routine processing?
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In which fixative do we see the use of Picric Acid and Glacial Acetic Acid together?
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Which fixative is known as 'Formol sublimate' and contains Chloride?
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At what concentration is Glutaraldehyde often used for small tissues?
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Study Notes
Fixatives
- Mercuric Chloride fixatives' primary function is to fix and preserve tissues.
- Formaldehyde fixatives have disadvantages, including being toxic and carcinogenic, and causing tissue shrinkage and hardening.
- A 4% concentration of formaldehyde is usually utilized for routine tissue fixation.
- Glutaraldehyde is often used for fixing Central Nervous System (CNS) tissues.
- Chromate Fixatives are used to preserve lipids and membranes.
- Osmic acid should not be used in Formaldehyde fixatives.
- Formaldehyde is known for preserving the nucleus and chromatin material of the cell.
- Acetic acid fixatives render the pH ≤4.6 and preserve membrane-bound organelles.
- Glacial acetic acid should not be used in Formaldehyde fixatives.
- Fat-containing fixatives, such as OsO4, are used to determine fat contents, presence of enzymes, and mucopolysaccharides.
- Formaldehyde is an aldehyde fixative.
- The most commonly utilized fixative in the laboratory is Formaldehyde.
- Phosphate buffers are used in formalin fixation to stabilize the pH and prevent tissue autolysis.
- Distilled water is used to dilute Formaldehyde to make a 10% solution for routine processing.
- Bouin's fixative uses Picric Acid and Glacial Acetic Acid together.
- Formol sublimate, which contains Chloride, is another name for Mercuric Chloride fixative.
- Glutaraldehyde is often used at a concentration of 2-3% for small tissues.
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Description
Test your knowledge on fixatives used in histopathology, including cytocological and histochemical fixatives. Learn about the different types of fixatives and their specific actions and remarks.
