Histopathology Fixatives Quiz
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Questions and Answers

What is the primary function of Mercuric Chloride fixatives?

  • Applied in Ultrathin Sections (correct)
  • Used for fixing CNS tissues
  • Mainly used in Enzyme Histochemistry
  • Utilized for sputum samples
  • What are the disadvantages of formaldehyde fixatives mentioned in the text?

  • Can cause allergic dermatitis (correct)
  • Commonly applied in Ultrathin Sections
  • May produce black granular deposits
  • Used for Enzyme Histochemistry
  • When is a concentration of 4% formaldehyde usually utilized?

  • For fixing CNS tissues
  • For fixing larger tissues (correct)
  • For fixing sputum samples
  • For Plastic Embedding
  • Which type of fixative is often used for Central Nervous System (CNS) tissues?

    <p>Lead Fixatives</p> Signup and view all the answers

    What is a common function of Chromate Fixatives?

    <p>Primarily used in Electron Microscopy</p> Signup and view all the answers

    Which fixative should not contain Osmic acid?

    <p>Heidenhain's Susa</p> Signup and view all the answers

    Which fixative is known for preserving the nucleus and chromatin material of the cell?

    <p>Flemming's Fixative</p> Signup and view all the answers

    Which fixative renders the pH ≤4.6 and preserves membrane-bound organelles?

    <p>Formol Corrosive</p> Signup and view all the answers

    Which fixative should not contain glacial acetic acid?

    <p>Glutaraldehyde</p> Signup and view all the answers

    Which fixative is used to determine fat contents, presence of enzymes, and mucopolysaccharides?

    <p>Newcomer's Bouin's Fixative</p> Signup and view all the answers

    Which fixative is an aldehyde fixative?

    <p>Formol Corrosive</p> Signup and view all the answers

    What is the most commonly utilized fixative in the laboratory?

    <p>Formalin</p> Signup and view all the answers

    What is the purpose of using phosphate buffers in formalin fixation?

    <p>To stabilize the pH at 7</p> Signup and view all the answers

    What component is used to dilute Formaldehyde to make a 10% solution for routine processing?

    <p>NaCl</p> Signup and view all the answers

    In which fixative do we see the use of Picric Acid and Glacial Acetic Acid together?

    <p>HISTO 10% Formol Saline</p> Signup and view all the answers

    Which fixative is known as 'Formol sublimate' and contains Chloride?

    <p>Orth's Regaud's Fixative</p> Signup and view all the answers

    At what concentration is Glutaraldehyde often used for small tissues?

    <p>2.5%</p> Signup and view all the answers

    Study Notes

    Fixatives

    • Mercuric Chloride fixatives' primary function is to fix and preserve tissues.
    • Formaldehyde fixatives have disadvantages, including being toxic and carcinogenic, and causing tissue shrinkage and hardening.
    • A 4% concentration of formaldehyde is usually utilized for routine tissue fixation.
    • Glutaraldehyde is often used for fixing Central Nervous System (CNS) tissues.
    • Chromate Fixatives are used to preserve lipids and membranes.
    • Osmic acid should not be used in Formaldehyde fixatives.
    • Formaldehyde is known for preserving the nucleus and chromatin material of the cell.
    • Acetic acid fixatives render the pH ≤4.6 and preserve membrane-bound organelles.
    • Glacial acetic acid should not be used in Formaldehyde fixatives.
    • Fat-containing fixatives, such as OsO4, are used to determine fat contents, presence of enzymes, and mucopolysaccharides.
    • Formaldehyde is an aldehyde fixative.
    • The most commonly utilized fixative in the laboratory is Formaldehyde.
    • Phosphate buffers are used in formalin fixation to stabilize the pH and prevent tissue autolysis.
    • Distilled water is used to dilute Formaldehyde to make a 10% solution for routine processing.
    • Bouin's fixative uses Picric Acid and Glacial Acetic Acid together.
    • Formol sublimate, which contains Chloride, is another name for Mercuric Chloride fixative.
    • Glutaraldehyde is often used at a concentration of 2-3% for small tissues.

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    Description

    Test your knowledge on fixatives used in histopathology, including cytocological and histochemical fixatives. Learn about the different types of fixatives and their specific actions and remarks.

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