Histopathology Fixatives Quiz

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Questions and Answers

What is the primary function of Mercuric Chloride fixatives?

  • Applied in Ultrathin Sections (correct)
  • Used for fixing CNS tissues
  • Mainly used in Enzyme Histochemistry
  • Utilized for sputum samples

What are the disadvantages of formaldehyde fixatives mentioned in the text?

  • Can cause allergic dermatitis (correct)
  • Commonly applied in Ultrathin Sections
  • May produce black granular deposits
  • Used for Enzyme Histochemistry

When is a concentration of 4% formaldehyde usually utilized?

  • For fixing CNS tissues
  • For fixing larger tissues (correct)
  • For fixing sputum samples
  • For Plastic Embedding

Which type of fixative is often used for Central Nervous System (CNS) tissues?

<p>Lead Fixatives (A)</p> Signup and view all the answers

What is a common function of Chromate Fixatives?

<p>Primarily used in Electron Microscopy (B)</p> Signup and view all the answers

Which fixative should not contain Osmic acid?

<p>Heidenhain's Susa (B)</p> Signup and view all the answers

Which fixative is known for preserving the nucleus and chromatin material of the cell?

<p>Flemming's Fixative (B)</p> Signup and view all the answers

Which fixative renders the pH ≤4.6 and preserves membrane-bound organelles?

<p>Formol Corrosive (B)</p> Signup and view all the answers

Which fixative should not contain glacial acetic acid?

<p>Glutaraldehyde (D)</p> Signup and view all the answers

Which fixative is used to determine fat contents, presence of enzymes, and mucopolysaccharides?

<p>Newcomer's Bouin's Fixative (D)</p> Signup and view all the answers

Which fixative is an aldehyde fixative?

<p>Formol Corrosive (C)</p> Signup and view all the answers

What is the most commonly utilized fixative in the laboratory?

<p>Formalin (A)</p> Signup and view all the answers

What is the purpose of using phosphate buffers in formalin fixation?

<p>To stabilize the pH at 7 (D)</p> Signup and view all the answers

What component is used to dilute Formaldehyde to make a 10% solution for routine processing?

<p>NaCl (A)</p> Signup and view all the answers

In which fixative do we see the use of Picric Acid and Glacial Acetic Acid together?

<p>HISTO 10% Formol Saline (C)</p> Signup and view all the answers

Which fixative is known as 'Formol sublimate' and contains Chloride?

<p>Orth's Regaud's Fixative (A)</p> Signup and view all the answers

At what concentration is Glutaraldehyde often used for small tissues?

<p>2.5% (A)</p> Signup and view all the answers

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Study Notes

Fixatives

  • Mercuric Chloride fixatives' primary function is to fix and preserve tissues.
  • Formaldehyde fixatives have disadvantages, including being toxic and carcinogenic, and causing tissue shrinkage and hardening.
  • A 4% concentration of formaldehyde is usually utilized for routine tissue fixation.
  • Glutaraldehyde is often used for fixing Central Nervous System (CNS) tissues.
  • Chromate Fixatives are used to preserve lipids and membranes.
  • Osmic acid should not be used in Formaldehyde fixatives.
  • Formaldehyde is known for preserving the nucleus and chromatin material of the cell.
  • Acetic acid fixatives render the pH ≤4.6 and preserve membrane-bound organelles.
  • Glacial acetic acid should not be used in Formaldehyde fixatives.
  • Fat-containing fixatives, such as OsO4, are used to determine fat contents, presence of enzymes, and mucopolysaccharides.
  • Formaldehyde is an aldehyde fixative.
  • The most commonly utilized fixative in the laboratory is Formaldehyde.
  • Phosphate buffers are used in formalin fixation to stabilize the pH and prevent tissue autolysis.
  • Distilled water is used to dilute Formaldehyde to make a 10% solution for routine processing.
  • Bouin's fixative uses Picric Acid and Glacial Acetic Acid together.
  • Formol sublimate, which contains Chloride, is another name for Mercuric Chloride fixative.
  • Glutaraldehyde is often used at a concentration of 2-3% for small tissues.

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