Podcast
Questions and Answers
What is the purpose of mixing the analyte solution with a matrix component before ionization?
What is the purpose of mixing the analyte solution with a matrix component before ionization?
- To protect the analyte molecules from the laser energy (correct)
- To evaporate the liquid
- To directly ionize the analyte molecules
- To absorb the laser energy
Which component is primarily ionized by the laser beam in the process described?
Which component is primarily ionized by the laser beam in the process described?
- Analyte molecules
- Matrix component (correct)
- Mass spectrometer
- Liquid solution
What is the role of the matrix component in transferring energy and charge to the analyte molecules during ionization?
What is the role of the matrix component in transferring energy and charge to the analyte molecules during ionization?
- To ionize the analyte directly
- To transfer energy and charge to the analyte (correct)
- To protect the analyte from laser energy
- To fragment the analyte molecules
Which type of mass spectrometer is typically used to analyze charged analytes in this process?
Which type of mass spectrometer is typically used to analyze charged analytes in this process?
In which ionization mode does Electron Impact (EI) result in the loss of a proton in negative mode?
In which ionization mode does Electron Impact (EI) result in the loss of a proton in negative mode?
What types of compounds are suitable for Electrospray Ionisation (ESI)?
What types of compounds are suitable for Electrospray Ionisation (ESI)?
Which type of compound is good for Matrix-Assisted Laser Desorption/Ionization (MALDI)?
Which type of compound is good for Matrix-Assisted Laser Desorption/Ionization (MALDI)?
What is the nature of the ionizing mechanism in Electrospray Ionisation (ESI) for negative mode?
What is the nature of the ionizing mechanism in Electrospray Ionisation (ESI) for negative mode?
Which ions are typically observed in Matrix-Assisted Laser Desorption/Ionization (MALDI)?
Which ions are typically observed in Matrix-Assisted Laser Desorption/Ionization (MALDI)?
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