Mass Spectrometry and Resolution Quiz
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Questions and Answers

What is the purpose of mixing the analyte solution with a matrix component before ionization?

  • To protect the analyte molecules from the laser energy (correct)
  • To evaporate the liquid
  • To directly ionize the analyte molecules
  • To absorb the laser energy

    • Which component is primarily ionized by the laser beam in the process described?

  • Analyte molecules
  • Matrix component (correct)
  • Mass spectrometer
  • Liquid solution

    • What is the role of the matrix component in transferring energy and charge to the analyte molecules during ionization?

  • To ionize the analyte directly
  • To transfer energy and charge to the analyte (correct)
  • To protect the analyte from laser energy
  • To fragment the analyte molecules

    • Which type of mass spectrometer is typically used to analyze charged analytes in this process?

    <p>Time-of-flight analyzer</p> Signup and view all the answers

    In which ionization mode does Electron Impact (EI) result in the loss of a proton in negative mode?

    <p>Negative mode loss of proton</p> Signup and view all the answers

    What types of compounds are suitable for Electrospray Ionisation (ESI)?

    <p>Non-polar, moderately polar; volatile and thermally stable</p> Signup and view all the answers

    Which type of compound is good for Matrix-Assisted Laser Desorption/Ionization (MALDI)?

    <p>Non-polar basic in gas phase to accept ionic compounds</p> Signup and view all the answers

    What is the nature of the ionizing mechanism in Electrospray Ionisation (ESI) for negative mode?

    <p>Loss of proton</p> Signup and view all the answers

    Which ions are typically observed in Matrix-Assisted Laser Desorption/Ionization (MALDI)?

    <p>$M^+$ ions</p> Signup and view all the answers

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