Introduction to Mass Spectrometry

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What is the main purpose of mass spectrometry?

Determining the molecular weight of a compound

Which part of a mass spectrometer is responsible for producing a beam of charged particles characteristic of the sample being analyzed?

Ion source

What is the role of deflection in mass spectrometry?

Separating ions based on their mass-to-charge ratios

In mass spectrometry, what happens during the acceleration stage?

Ions are sped up to have the same kinetic energy

What principle governs the sorting of gaseous ions in mass spectrometry?

Mass-to-charge ratio principle

Why is the detection step crucial in mass spectrometry?

To identify deflected ions and determine their mass-to-charge ratio

Study Notes

Mass Spectrometry

  • Mass spectrometry is an analytical technique that involves ionization and fragmentation of molecules for mass and structural analysis.
  • It consists of five basic parts: high vacuum system, sample handling system, ion source, analyzer, and detector.

Principles of Mass Spectrometry

  • The principle of mass spectrometry involves sorting of gaseous ions in electric and magnetic fields according to their mass-to-charge ratios.
  • Instruments used in mass spectrometry studies are called mass spectrometers and mass spectrographs.

Steps in Mass Spectrometry

  • Ionization: conversion of sample into charged particles.
  • Acceleration: speeding up ions to give them the same kinetic energy.
  • Deflection: deflecting ions with a magnetic field, with lighter ions deflecting more.
  • Detection: detecting deflected ions and determining their mass-to-charge ratio.

Learn about the analytical technique of mass spectrometry, which involves the ionization and fragmentation of molecules for mass and structural analysis. Explore the five basic parts of a mass spectrometer and understand its applications in research and analysis.

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