Introduction to Mass Spectrometry
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Introduction to Mass Spectrometry

Learn about the analytical technique of mass spectrometry, which involves ionization and fragmentation of molecules for mass and structural analysis. Explore the five basic parts of mass spectrometry and understand its importance in scientific research.

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Questions and Answers

Mass spectrometry involves the ionization and fragmentation of molecules for size and structural analysis.


Mass spectrometry consists of four basic parts: a high vacuum system, a sample handling system, an ion source, and an analyzer.


The principle of mass spectrometry involves sorting gaseous ions in electric fields based on their mass-to-charge ratios.


Mass spectrometers and mass spectographs are used to study the principles of mass spectrometry.

<p>True</p> Signup and view all the answers

During ionization in mass spectrometry, the sample is converted into neutral particles.

<p>False</p> Signup and view all the answers

In mass spectrometry, the ions are deflected by an electric field, where the lighter ions are more deflected.

<p>False</p> Signup and view all the answers

Detection in mass spectrometry involves determining the charge of ions rather than their mass-to-charge ratio.

<p>False</p> Signup and view all the answers

Study Notes

Introduction to Mass Spectrometry

  • Mass spectrometry is an analytical technique used for mass and structural analysis of molecules.
  • It involves the ionization and fragmentation of molecules.

Components of Mass Spectrometry

  • Five basic parts:
    • High vacuum system
    • Sample handling system for introducing the sample
    • Ion source for producing a beam of charged particles
    • Analyzer for separating the beam into its components
    • Detector

Principles of Mass Spectrometry

  • Sorting of gaseous ions in electric and magnetic fields according to their mass-to-charge ratios.
  • Instruments used: mass spectrometers and mass spectographs.

Ionization and Fragmentation Process

  • Ionization: conversion of the sample into charged particles.
  • Acceleration: speeding up ions to give them the same kinetic energy.
  • Deflection: deflection of ions by a magnetic field, with lighter ions deflected more.
  • Detection: detection of deflected ions and determination of mass-to-charge ratio.

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