Marine Biotechnology Unit 1 Quiz
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Questions and Answers

What characterizes secondary metabolites compared to primary metabolites?

  • They are dependent on environmental conditions. (correct)
  • They are larger and chemically less diverse.
  • They accumulate without any regulation.
  • They are synthesized through linear pathways only.
  • What occurs when metabolic regulation is efficient in organisms?

  • Certain steps of primary metabolism have regulated output. (correct)
  • Secondary metabolites are overproduced.
  • Intermediate products accumulate.
  • Primary pathways produce terminal products without regulation.
  • What can induce subsidiary pathways to form secondary metabolites in organisms?

  • Metabolic stress from intermediate accumulation. (correct)
  • High concentrations of primary metabolites.
  • Optimal temperature and light levels.
  • Sufficient nutrient availability.
  • How do new metabolites arise through genetic changes in organisms?

    <p>By random recombination and mutation. (A)</p> Signup and view all the answers

    What happens when a new compound proves advantageous for an organism?

    <p>The biosynthetic capacity becomes imprinted. (A)</p> Signup and view all the answers

    What is the role of combinatorial biology in natural product discovery?

    <p>It generates new genes mixed with pathway genes for diverse products. (B)</p> Signup and view all the answers

    Which of the following pathways often lead to the formation of macrocyclic antibiotics?

    <p>Fatty acid and monosaccharide biosynthetic pathways. (B)</p> Signup and view all the answers

    Which pigment is responsible for the brown color of Phaeophyta?

    <p>Fucoxanthin (A)</p> Signup and view all the answers

    What class of compounds do the natural products of cyanobacteria NOT include?

    <p>Amino acids (C)</p> Signup and view all the answers

    How do the cell walls of brown algae primarily differ from those of green algae?

    <p>Brown algae walls contain alginic acid, while green algae walls do not (C)</p> Signup and view all the answers

    Which of the following uses is NOT associated with the natural products derived from cyanobacteria?

    <p>Antidepressants (C)</p> Signup and view all the answers

    How many genera are approximately contained within the brown algae group (Phaeophyta)?

    <p>265 (A)</p> Signup and view all the answers

    What is the primary advantage of silicon spicules found in sponges over optical fibers?

    <p>They avoid the self-shading effect in microalgae cultivation. (B)</p> Signup and view all the answers

    Which of the following factors is NOT mentioned as affecting microalgae growth and metabolite accumulation?

    <p>Salinity (D)</p> Signup and view all the answers

    Why are products derived from sponges often synthesized artificially?

    <p>Sponge cultures require complex and costly systems. (C)</p> Signup and view all the answers

    In which phase of microalgae growth is it preferable to harvest?

    <p>Exponential phase (B)</p> Signup and view all the answers

    What is the habitat preference of Cnidarians as described?

    <p>Aquatic environments, preferably rocky shores (A)</p> Signup and view all the answers

    Which of the following is a characteristic of Cyclopeptides isolated from Geodia barretti?

    <p>They deter barnacle larvae settlement. (A)</p> Signup and view all the answers

    What biomass range is noted for Cnidarians?

    <p>8 to 15 grams/liter (D)</p> Signup and view all the answers

    How can cropping systems of microalgae be illuminated?

    <p>By both artificial light and sunlight. (D)</p> Signup and view all the answers

    What is the purpose of vigorous mixing in microalgae cultivation?

    <p>To ensure high frequency light:dark cycles. (B)</p> Signup and view all the answers

    What is a common characteristic of Cnidarians in terms of their species diversity?

    <p>There are about 10,000 species. (A)</p> Signup and view all the answers

    What is the optimum temperature range for growing most microalgae?

    <p>20 to 24 ºC (D)</p> Signup and view all the answers

    What pH range is generally optimal for the growth of most microalgae?

    <p>8.2 to 8.7 (D)</p> Signup and view all the answers

    At what temperature is the growth of many microalgae species considered lethal?

    <p>35 ºC (C)</p> Signup and view all the answers

    What happens to carotenoid accumulation in some algae when exposed to high temperatures?

    <p>It can increase at temperatures up to 40 ºC. (C)</p> Signup and view all the answers

    Why is an initial pH adjustment to 6.5 recommended prior to inoculation?

    <p>To support optimal CO2 availability. (B)</p> Signup and view all the answers

    How does increasing the age of a microalgae crop affect pH levels?

    <p>It causes pH to increase due to mineral accumulation. (C)</p> Signup and view all the answers

    What is the effect of excess mechanical agitation in algae cultivation?

    <p>It can permanently damage cell structures. (B)</p> Signup and view all the answers

    What is the role of buffer solutions in microalgae cultivation?

    <p>To maintain desired pH levels. (B)</p> Signup and view all the answers

    What is the consequence of using alkaline pH levels in microalgae culture?

    <p>It can limit CO2 availability. (C)</p> Signup and view all the answers

    Which of these factors affects the absorption efficiency in Chlorella zofingiensis?

    <p>Temperature and pigment levels. (C)</p> Signup and view all the answers

    What is the primary method by which marine sponges obtain their nutrients?

    <p>By capturing water through external pores (A)</p> Signup and view all the answers

    How much water can even the smallest sponge filter in a day?

    <p>20 litres (D)</p> Signup and view all the answers

    Which type of sponge is known for releasing cytotoxic substances to compete for resources?

    <p>Both encrusting and tree-like sponges (D)</p> Signup and view all the answers

    What was one of the first synthetic drugs derived from marine sponge compounds?

    <p>Zovirax (Acyclovir) (B)</p> Signup and view all the answers

    What unique property does rosacelose have?

    <p>Anti-HIV activity (D)</p> Signup and view all the answers

    Which sponge has shown extraordinary potential as an anti-tumor agent?

    <p>Halicondria okadai (D)</p> Signup and view all the answers

    What type of activities are metabolites from the genus Haliclona known for?

    <p>Antifouling, antimicrobial, antifungal, and antimalarial activity (D)</p> Signup and view all the answers

    Which compound was isolated in the 1950 studies to mark the beginning of marine therapeutic agent research?

    <p>Spongothymidine (B)</p> Signup and view all the answers

    What is a potential application of the cytotoxic substances released by sponges?

    <p>Anti-tumor drugs (C)</p> Signup and view all the answers

    Flashcards

    What are secondary metabolites?

    Secondary metabolites are smaller and more diverse than primary metabolites, such as proteins, lipids, carbohydrates, and nucleic acids. They are generated by branched pathways of primary metabolism.

    How are secondary metabolites affected by environment?

    Secondary metabolites are influenced by the environment. Production can be regulated by factors like temperature, salinity, light, and water hardness.

    How are secondary metabolites produced in organisms with efficient metabolic regulation?

    In efficient metabolic regulation, primary metabolic pathways don't accumulate intermediates and terminal products. However, certain organisms have genes for secondary metabolite production, leading to an overproduction of intermediates in primary pathways.

    What is the role of metabolic stress in secondary metabolite production?

    Under metabolic stress, these accumulated intermediates can trigger the formation of secondary metabolites. For example, algae subjected to stress can produce secondary metabolites.

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    How do pathways combine to create secondary metabolites?

    Combining fatty acid biosynthetic pathways with monosaccharide pathways can result in the formation of macrocycles, like antibiotics or macrolides.

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    How do secondary metabolic pathways evolve?

    Secondary metabolic pathways arise through random recombination and mutation of existing genes. If the new compound provides advantages, this biosynthetic capability is reinforced.

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    How are natural products discovered through combinatorial biology?

    Genetic processes are combined with bioassays to identify and isolate recombinant clones, potentially producing novel natural products with biological activities against diseases.

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    How do marine sponges feed?

    Marine sponges feed through pores on their bodies, filtering water and absorbing nutrients.

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    What is the ecological role of sponges?

    They play an important role in keeping marine waters clean by filtering out pollutants.

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    What makes sponges valuable from a scientific perspective?

    Sponges produce a wide range of unique compounds, many with potential medicinal uses.

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    Why do sponges create secondary metabolites?

    Sponges produce secondary metabolites to defend themselves and compete for resources.

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    How are sponges used in cancer research?

    Sponges like Haliclona permollis produce cytotoxic substances, which are compounds capable of killing cancer cells.

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    What is the historical significance of sponge research?

    In the 1950s, scientists isolated nucleosides from sponges, leading to the development of anti-tumour and antiviral drugs.

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    What is Ara-C and what is it used for?

    Ara-C, a drug derived from sponge compounds, is a crucial anti-tumour agent.

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    What is Zovirax and what is it used for?

    Zovirax, another drug derived from sponge compounds, effectively inhibits viral replication.

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    What is rosacelose and what is its potential?

    A compound called rosacelose extracted from the Myxilla rosacea sponge shows promise in combating HIV.

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    What are sponges?

    Sponges are marine organisms with unique properties, including the production of bioactive compounds with potential applications in medicine, agriculture, and environmental protection.

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    What are examples of sponge bioactive compounds?

    Anti-fouling compounds produced by sponges help prevent the growth of organisms like barnacles and mussels on their surfaces.

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    What are cnidarians?

    Cnidarians, such as corals, jellyfish, and anemones, are aquatic animals with stinging cells called nematocysts.

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    What are microalgae?

    Microalgae, like cyanobacteria, are single-celled photosynthetic organisms that play a crucial role in the food chain and produce valuable biomolecules.

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    What factors affect microalgae growth?

    The growth and production of metabolites in microalgae are influenced by factors such as light intensity, CO2 availability, and temperature.

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    What are raceways?

    Microalgae are often grown in raceways, which are shallow, open ponds designed to maximize their exposure to sunlight and nutrients.

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    Why is light intensity important for microalgae ?

    Light intensity is a critical factor for the growth of microalgae, as it provides the energy for photosynthesis.

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    What are different ways to illuminate microalgae?

    Microalgae cultures can be illuminated by artificial light, natural sunlight, or a combination of both depending on the cultivation strategy.

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    When is the ideal time to harvest microalgae?

    The harvesting of microalgae is typically done at the exponential phase of growth, maximizing biomass production.

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    What is the death phase in microalgae cultivation?

    The death phase in microalgae cultivation represents the decline in growth and the possibility of cell lysis, which can impact production and potential contamination.

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    What are Brown algae?

    Brown algae are classified in the division Phaeophyta and are characterized by a wide range of morphologies, from simple filamentous structures to complex, large algae.

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    What gives brown algae their colour?

    Fucoxanthin, a xanthophyll pigment, gives brown algae their distinctive colour. This pigment acts as a shield from strong sunlight.

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    What are the components of brown algae's cell wall?

    Brown algae have cell walls composed of cellulose and alginic acid. Alginic acid is an important ingredient in various biotechnology applications.

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    What characterizes Green algae?

    Green algae belong to the Chlorophyta division and are known for their bright green colour due to the presence of chlorophyll a and b.

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    How do Green algae store energy and what are their cell walls made of?

    Green algae store carbohydrates in the form of starch and have cell walls primarily made of cellulose. They are the most diverse group of algae with over 10,000 species.

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    Optimal temperature for microalgae growth

    The optimal range for growing microalgae is between 20 and 24 degrees Celsius (68-75 degrees Fahrenheit).

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    Tolerance range of microalgae

    Most microalgae can tolerate a slightly wider temperature range, from 16 to 27 degrees Celsius (61-81 degrees Fahrenheit).

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    Harmful temperature for microalgae

    Temperatures above 35 degrees Celsius (95 degrees Fahrenheit) can be lethal to many microalgae species, causing them to die.

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    Temperature impact on microalgae metabolism

    Changes in temperature can impact the metabolic pathways of microalgae, including the production of carotenoids.

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    Species-specific temperature preferences

    The specific species and strain of microalgae can influence the optimal temperature range for growth.

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    Optimal pH range for microalgae

    The ideal pH range for most microalgae is between 7 and 9, with an optimal range of 8.2 to 8.7, slightly alkaline.

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    pH increase in high density cultures

    High cell density cultures can lead to increased pH due to mineral accumulation and nutrient oxidation.

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    Controlling pH with CO2

    Aeration with CO2-enriched air can help adjust and maintain the optimal pH in microalgae cultures.

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    Importance of mixing in microalgae cultivation

    Efficient mixing is crucial in microalgae cultivation to ensure uniform distribution of algae, light, nutrients, and temperature.

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    Potential harm of excessive agitation

    Excessive mechanical agitation can damage microalgae cells, negatively impacting their growth and metabolite production.

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    Study Notes

    Unit 1: Introduction to Marine Biotechnology

    • Blue Biotechnology is the application of science and technology to living aquatic organisms for knowledge, goods, and services (OECD, 2016).
    • Natural products from terrestrial sources currently make up over 50% of the market, but these resources are being exploited and depleted.
    • The oceans are a rich source of biological and chemical diversity, containing over 300,000 documented species of plants and animals.
    • Marine organisms have unique properties, often differing from terrestrial organisms due to their exclusively marine environment, including adaptations like gas vesicles in algae for buoyancy and light capture.
    • Marine organisms possess various bioactive metabolites and natural products, frequently having novel chemical structures.
    • Rigid terrestrial plants differ in cell wall structure from flexible marine plants (e.g., gas vesicles in Fucus vesiculosus).
    • Cell wall of brown algae can act as ion exchangers with high capacity, 3.5 times greater than in terrestrial plants.

    Unit 1: Index

    • Marine biodiversity and blue biotechnology (page 1)
    • Marine organisms vs. terrestrials (page 1)
    • Research in Marine Biotechnology (page 2)
    • Natural products or secondary metabolites? (page 3)
    • Bioprospection of the marine environment and NMP screening techniques (page 7)

    Unit 4: Marine Biotechnology and the Environment

    • Treatment technology overview (page 81)
    • Bioremediation overview (page 83)
    • Biosensors (page 85)
    • Biomarkers (page 86)

    Unit 2: Biotechnological Applications from Marine Organisms

    • Index of topics (page 14)
    • Sponges (pages 16-18)
    • Cnidarians (pages 21-23)
    • Annelids (page 26)
    • Molluscs (page 30)
    • Lophophores (page 33)
    • Crustaceans (page 21)
    • Echinoderms (pages 41-43)
    • Tunicates (pages 44-45)

    Unit 3: Microalgae Biotechnology Applications

    • Index of topics (page 52)
    • Microalgae (page 53)
    • Photobioreactors (page 54)
    • Microalgae cultivation (page 58)
    • Cyanobacteria (page 62)
    • Biotechnology applications of cyanobacteria (page 62)

    Unit 3.2: Biotechnological Applications of Macroalgae

    • Index of topics (page 67)
    • Macroalgae (page 68)
    • Importance of macroalgae culture (page 69)
    • Macroalgae culture (page 71)
    • Biotechnological applications of macroalgae (page 78)

    Unit 5: Food Security and Marine Biotechnology (Aquaculture)

    • Index of topics (page 88)
    • Importance of aquaculture (page 89)
    • Biotechnology and aquaculture (page 90)
    • Reproduction induction (page 91)
    • Sex control (page 92)
    • Chromosomic manipulation (page 93)
    • Genetic selection programs (page 94)

    Unit 6: Biomaterials and Bioplastics

    • Index of topics (page 97)
    • What is a biomaterial? (page 98)
    • Importance of biomaterials (page 99)
    • Types of biomaterials (page 101)
    • Bioplastics (page 101)
    • Biomaterials of marine origin (page 102)

    Unit 7: Expectations of Research in Marine Biotechnology

    • Index of topics (page 104)
    • Marine biotechnology as a discipline (page 105)
    • Expansion of marine biotechnology (page 106)
    • Marine biotechnology and public policy (page 108)

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    Description

    Test your knowledge on the fundamentals of marine biotechnology in this quiz about Unit 1. Explore the unique properties of marine organisms and their applications in science and technology. Understand the significance of marine biodiversity and its potential for sustainable resources.

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