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Mannitol Salt Agar Lab Reading & Procedure

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Mannitol Salt Agar Lab Reading & Procedure

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Questions and Answers

What color does the pH indicator turn when encountering acid on the mannitol salt agar plate?

Bright pink

Bright orange

Bright red

Bright yellow (correct)

How does the appearance of the non-mannitol fermenter differ from the mannitol fermenter on the agar surface?

It grows more rapidly

It turns pink

It develops a green color

It shows no color change of the agar (correct)

What is the role of sodium chloride in mannitol salt agar?

Enhances the growth of Staphylococcal species

Prevents the growth of unwanted Gram-negative organisms (correct)

Turns the agar yellow

Promotes the growth of Gram-negative organisms

Why is it important for mannitol salt agar to inhibit the growth of Gram-negative organisms?

They compete for resources with Staphylococcal species Signup and view all the answers

In what way does the yellow halo on the agar indicate the spread of acid from mannitol fermentation?

The halo indicates how far acid has reached from fermentation Signup and view all the answers

What is the purpose of Mannitol Salt Agar?

To select for and differentiate among Gram-positive Staphylococcus species Signup and view all the answers

Why is a pH indicator added to Mannitol Salt Agar plates?

To differentiate organisms based on their metabolic capabilities Signup and view all the answers

How does Mannitol Salt Agar differentiate among different organisms?

By their ability to produce acid during fermentation Signup and view all the answers

What makes Mannitol Salt Agar a differential media?

The ability to differentiate among organisms based on their metabolic characteristics Signup and view all the answers

Why is Mannitol important in Mannitol Salt Agar?

It is the ingredient that makes the media differential Signup and view all the answers

Study Notes

Mannitol Salt Agar Plate

When a Gram-positive organism capable of mannitol fermentation is inoculated on the plate, it turns the pink agar yellow due to acid production.

Characteristics of Mannitol Salt Agar

Selective and differential medium
Specifically selects for and promotes growth of Gram-positive Staphylococcus species
Contains mannitol, a carbohydrate that some organisms can ferment while others cannot
Contains a pH indicator that changes the agar color in response to acid production

Role of Sodium Chloride (Salt)

Inhibits the growth of unwanted Gram-negative organisms
Makes mannitol salt agar selective for Gram-positive Staphylococcus species
Staphylococcal species can live in salty environments, such as on human skin

pH Indicator and Mannitol Fermentation

When an organism ferments mannitol, it produces acid as a waste product
The acid diffuses through the agar, decreasing the pH and turning the agar yellow
The yellow halo surrounding the organism indicates the spread of acid from mannitol fermentation

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Description

Explore the reading and procedure of Mannitol Salt Agar, a selective and differential media used in medical microbiology labs. Learn about how this type of media assists in identifying unknown organisms by selecting for specific types and differentiating among different species.