Mammalian Cell Culture
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Questions and Answers

What is the typical duration for mammalian cells to complete replication?

  • 40-50 hours
  • 30-40 hours
  • 12-18 hours
  • 22-30 hours (correct)
  • What is the primary purpose of adding FBS to cell culture media?

  • To provide growth factors (correct)
  • To adjust the pH of the media
  • To increase the surface tension of the media
  • To prevent bacterial growth
  • What is the primary characteristic of single-cell organisms?

  • They are only found in multicellular organisms
  • They are composed of a single cell (correct)
  • They are formed by multiple rounds of cell division
  • They are generated from multiple cells
  • What is the main difference between single-cell and multicellular organisms?

    <p>The number of cells they are composed of</p> Signup and view all the answers

    What is the ideal temperature for culturing mammalian cells?

    <p>37°C</p> Signup and view all the answers

    What is the term for the study of the structure and function of cells?

    <p>Cytology</p> Signup and view all the answers

    Who is credited with coining the term 'cell'?

    <p>Robert Hooke</p> Signup and view all the answers

    What is the term for the process by which cells are generated from a single cell?

    <p>Cell division</p> Signup and view all the answers

    Why is it essential to check cell culture media for contamination every time before use?

    <p>To detect any potential bacterial or fungal contamination</p> Signup and view all the answers

    What is the primary method of sterilization for mammalian cell culture media?

    <p>Filtration</p> Signup and view all the answers

    What type of plastic materials are typically pre-sterilized by manufacturers?

    <p>Culture plates, flasks, and tubes</p> Signup and view all the answers

    What is the term for the controlled growth of cells in a laboratory?

    <p>Cell culture</p> Signup and view all the answers

    What is the primary function of the carbohydrate layer on the cell surface?

    <p>To protect the cell surface from mechanical and chemical damage</p> Signup and view all the answers

    What is the typical temperature at which mammalian cells are cultured?

    <p>37°C</p> Signup and view all the answers

    What is the purpose of adding fetal bovine serum (FBS) to the culture medium?

    <p>To provide growth factors and antibiotics</p> Signup and view all the answers

    How often do most mammalian cells replicate?

    <p>Every 22-30 hours</p> Signup and view all the answers

    What is the typical pH of the culture medium used for mammalian cells?

    <p>7.3</p> Signup and view all the answers

    What is a key factor in selecting a suitable culture medium for a particular cell line?

    <p>The specific growth factors required by the cell line</p> Signup and view all the answers

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