Mammalian Cell Culture

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Questions and Answers

What is the typical duration for mammalian cells to complete replication?

  • 40-50 hours
  • 30-40 hours
  • 12-18 hours
  • 22-30 hours (correct)

What is the primary purpose of adding FBS to cell culture media?

  • To provide growth factors (correct)
  • To adjust the pH of the media
  • To increase the surface tension of the media
  • To prevent bacterial growth

What is the primary characteristic of single-cell organisms?

  • They are only found in multicellular organisms
  • They are composed of a single cell (correct)
  • They are formed by multiple rounds of cell division
  • They are generated from multiple cells

What is the main difference between single-cell and multicellular organisms?

<p>The number of cells they are composed of (B)</p> Signup and view all the answers

What is the ideal temperature for culturing mammalian cells?

<p>37°C (D)</p> Signup and view all the answers

What is the term for the study of the structure and function of cells?

<p>Cytology (C)</p> Signup and view all the answers

Who is credited with coining the term 'cell'?

<p>Robert Hooke (B)</p> Signup and view all the answers

What is the term for the process by which cells are generated from a single cell?

<p>Cell division (D)</p> Signup and view all the answers

Why is it essential to check cell culture media for contamination every time before use?

<p>To detect any potential bacterial or fungal contamination (B)</p> Signup and view all the answers

What is the primary method of sterilization for mammalian cell culture media?

<p>Filtration (D)</p> Signup and view all the answers

What type of plastic materials are typically pre-sterilized by manufacturers?

<p>Culture plates, flasks, and tubes (B)</p> Signup and view all the answers

What is the term for the controlled growth of cells in a laboratory?

<p>Cell culture (A)</p> Signup and view all the answers

What is the primary function of the carbohydrate layer on the cell surface?

<p>To protect the cell surface from mechanical and chemical damage (B)</p> Signup and view all the answers

What is the typical temperature at which mammalian cells are cultured?

<p>37°C (D)</p> Signup and view all the answers

What is the purpose of adding fetal bovine serum (FBS) to the culture medium?

<p>To provide growth factors and antibiotics (A)</p> Signup and view all the answers

How often do most mammalian cells replicate?

<p>Every 22-30 hours (D)</p> Signup and view all the answers

What is the typical pH of the culture medium used for mammalian cells?

<p>7.3 (B)</p> Signup and view all the answers

What is a key factor in selecting a suitable culture medium for a particular cell line?

<p>The specific growth factors required by the cell line (C)</p> Signup and view all the answers

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