18 Questions
What is the typical duration for mammalian cells to complete replication?
22-30 hours
What is the primary purpose of adding FBS to cell culture media?
To provide growth factors
What is the primary characteristic of single-cell organisms?
They are composed of a single cell
What is the main difference between single-cell and multicellular organisms?
The number of cells they are composed of
What is the ideal temperature for culturing mammalian cells?
37°C
What is the term for the study of the structure and function of cells?
Cytology
Who is credited with coining the term 'cell'?
Robert Hooke
What is the term for the process by which cells are generated from a single cell?
Cell division
Why is it essential to check cell culture media for contamination every time before use?
To detect any potential bacterial or fungal contamination
What is the primary method of sterilization for mammalian cell culture media?
Filtration
What type of plastic materials are typically pre-sterilized by manufacturers?
Culture plates, flasks, and tubes
What is the term for the controlled growth of cells in a laboratory?
Cell culture
What is the primary function of the carbohydrate layer on the cell surface?
To protect the cell surface from mechanical and chemical damage
What is the typical temperature at which mammalian cells are cultured?
37°C
What is the purpose of adding fetal bovine serum (FBS) to the culture medium?
To provide growth factors and antibiotics
How often do most mammalian cells replicate?
Every 22-30 hours
What is the typical pH of the culture medium used for mammalian cells?
7.3
What is a key factor in selecting a suitable culture medium for a particular cell line?
The specific growth factors required by the cell line
Learn about the process of mammalian cell culture, including the necessary conditions, media, and equipment. Understand the importance of a sterile environment and the role of growth factors and antibiotics. Test your knowledge of cell culture techniques and protocols.
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