Mammalian Cell Culture Overview

Podcast

Play an AI-generated podcast conversation about this lesson

Questions and Answers

Quale test es essential pro assecurar que le medium non es compromittite post filtration?

Verificar si le membrana del filtro es intacte (correct)

Evaluar le sapor del medium

Assicurarse que le pH del medium es correct

Testar si le medium es colorate

Que significa 'Plating efficiency' in le contexto de cultura cellulare?

Le proportion de cellul que cresce in colonias de celles in 100 celles inoculate (correct)

Le velocitate de croissance del celles durante le incubation

Le numero de fois que le celles es transferite a un novo medium

Le quantitate de medium usate pro cultiva cellulapor

Quale de le sequente es un test de sterilisazione pro le medium?

Transferimento de celles a un novo receptacle

Observation del color del medium

Misura del pH del medium

Incubation de aliquotas de medium in varios broths (correct)

Que es le metodo pro comparar le characteristics de cultura cellular inter le novos e vetustos lots de medium?

Compare le curvas de croissance Signup and view all the answers

Quando un medium es preparate in le laboratorio, que tipo de test debe esser realizate ante le uso?

Testo de promtion de croissance e sterilitá Signup and view all the answers

Quande se discute le media de cultura cellular mammalian?

In le octave et novem septiman Signup and view all the answers

Qual de iste non es un metodo de sterilisation pro media de cultura cellular?

Sterilisation per radiomembrana Signup and view all the answers

Qual es un control de qualitate que debe esser facte ante le uso de media?

Controllo del pH Signup and view all the answers

Quande se face un recap de lecturas ante le examenes?

In le undecime et duodecime septiman Signup and view all the answers

Qual lection recuntrara le biologia del cellulas de cultura?

Leccion 8 Signup and view all the answers

Qual es un tema discutite in le lection 15?

Response immunitaria innate Signup and view all the answers

Qual de iste non es un parte del programma de lection?

Culture de cellulas vegetales Signup and view all the answers

Qual es le methodo de sterilisation que non es necessario si le media es preparate in un ambiente aseptic?

Filtration per membrana Signup and view all the answers

Qual broth es usate pro isolar organisimos aerobic e facultative?

TSB Signup and view all the answers

Quo es le principal causa pro sterilisar le media pro cultura cellular?

Proteger le media ab contamination Signup and view all the answers

Quo es un signale de contamination in un sterility test?

Cloudiness Signup and view all the answers

Qual es le temperature correct pro incubar TSB media durante le test de sterility?

37 C Signup and view all the answers

Quale de le sequente techniques es crucial pro mantener le sterilidade del media?

Technique aseptica Signup and view all the answers

Pro quantos dies deberea le SDB media essere incubate in le test de sterility?

5 dies Signup and view all the answers

Quale es le methodo preferite pro sterilisar media pro cultura cellular?

Filtration Signup and view all the answers

Qual broth es usate pro isolar organisimos anaerobico?

TGB Signup and view all the answers

Quale es le pore size recomendate pro le filtrage steril del media?

0.2µm Signup and view all the answers

Qual es le prime passo ante sterilisation de media?

Dissolver in aqua purificate Signup and view all the answers

Quale de le sequente materiales debe esser inert pro un filter sterilizante?

Inoxidable Signup and view all the answers

Quale effetto debe esser confirmate per le studi de crescimento post-filtrage?

Nullo effetto super le proprietates de promovimento del crescimento Signup and view all the answers

Qual es le procedura pro verificar le sterility de un media?

Incubate e observar pro crescita Signup and view all the answers

Quo debe ocurrer si contamination es observate in un batch?

Discarder le batch Signup and view all the answers

Quale de le sequente es un requirement pro le containers de media?

Debent esser steril Signup and view all the answers

Quo non es un criterium pro le media components?

Costo basse Signup and view all the answers

Qual es le fontanian de aqua considerat le plus pur?

Aqua pro injection Signup and view all the answers

Quale de le sequente non es un methodo de purification de aqua?

Ajustamento de pH Signup and view all the answers

Porque aqua potabile debe subire purificazione in-domicilio ante utiliser?

Illa ha contaminantes organicos Signup and view all the answers

Qual parte del processu de purificazione elimina microorganismos?

Filtration Signup and view all the answers

Qual es un detail importante a documentar durante le preparation de media?

Le materiaes prime usate Signup and view all the answers

Quale de le sequente describe aqua purificate?

Le aqua qui ha subite processu de filtration Signup and view all the answers

Qual es le standard US que determina le criteria pro aqua pro injection?

USP Signup and view all the answers

Qual contaminante es eliminat per 'Activated Charcoal'?

Material organico Signup and view all the answers

Study Notes

Week Schedule

Week 1: Lecture Module Introduction, Tuesday, September 10th
Week 2: Lecture 1 (Use of Mammalian Cells), Monday, September 16th; Lecture 2 (Cell Culture Lab Layout, Equipment, and Materials), Tuesday, September 17th
Week 3: Lecture 3 (Contamination Control), Monday, September 23rd; Lecture 4 (Contamination Control), Tuesday, September 24th
Week 4: Lecture 5 (Contamination Control), Monday, September 30th; Recap of Lectures 2, 3, 4, and 5, and sample assessment questions, Tuesday, October 1st
Week 5: Lecture 6 (Nutrient Uptake), Monday, October 7th; Lecture 7 (Nutrient Uptake), Tuesday, October 8th
Week 6: Lecture 8 (Biology of Culture Cells), Monday, October 14th; Lecture 9 (Cell Culture Media), Tuesday, October 15th
Week 7: Lecture 10 (Cell Culture Media), Monday, October 21st; Lab 3 Data Analysis, Tuesday, October 22nd
Week 8: Reading Week
Week 9: Lecture 10 (Cell Culture Media), Monday, November 4th; Lecture 11 (Cell Culture Media), Tuesday, November 5th; Lecture 12 (Growing Mammalian Cells), Monday, November 11th; Lecture 8, 9, 10, and 11 recap and sample assessment questions, Tuesday, November 12th
Week 10: Lecture 13 (Monitoring Growth), Monday, November 18th; Lecture 14 (Cryopreservation), Tuesday, November 19th
Week 11: Lecture 15 (Innate Immune Response), Monday, November 25th; Lecture 16 (Adaptive Immune Response and Bioassays), Tuesday, November 26th; Recap of Lectures 12, 13, and 14, and sample assessment questions
Week 12: Lecture 15 and 16 recap and sample assessment questions, revision, Monday, December 2nd; Revision, Tuesday, December 3rd

Mammalian Cell Culture Media - Lecture Overview

Introduction: Why discuss this topic
Main discussion: Mammalian cell culture media, sterilization and quality control
Conclusion: Take home message

Mammalian Cell Culture Media - Sterility

Cell culture media need to be free of harmful contaminants and adventitious biological agents (bacteria, fungi, viruses, mycoplasma).
Sterility relies on laminar flow biological safety cabinets / cleanrooms, suitable work surfaces, trained personnel, quality controlled reagents and media, and meticulous aseptic technique and equipment maintenance.

Mammalian Cell Culture Media - Sterilization Methods

Membrane filtration is the preferred method for sterilizing cell culture media.
Autoclaving is generally not suitable as it could destroy critical components.
Sterile filters are rated for 0.2µm or smaller.
Filter materials used must be inert and not add or remove components.

Quality Control of Cell Culture Media

Growth Promotion: Evaluate growth characteristics by comparing old and new media batches.
Sterility Tests: Standard microbiological tests for bacteria/yeasts, incubating aliquots of media in different broths (e.g., Trypticase Soy Broth), incubating and observing for growth.
Sterile Filter: Ensure filter membrane integrity is tested to confirm intact membrane after filtration.

Water Purification for Cell Culture Media

Components of cell culture media are dissolved in purified water.
Potable water is subjected to further in-house purification prior to use.
Prepared media are sterilized by filtration and tested for sterility
Preparation details should be recorded, and an expiry date assigned to the batch.

Water for Injection (WFI)

Water for injection (WFI) is the purest form of water for use in medicinal applications.
Distinguished from purified water based on the range/quantity of allowable contaminants.

Studying That Suits You

Use AI to generate personalized quizzes and flashcards to suit your learning preferences.

Description

Este quiz explora le structure e metodologia pro le cultura de cellulas mammaliane. Le focus es on contamination control e uptake de nutriente durant le processus. Examina le lecturas e le assessement que se basano in iste topics crucial.