Cell Culture Techniques Overview

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Questions and Answers

Which type of transport does not require energy from the cell?

Osmosis (correct)

Facilitated Diffusion (correct)

Endocytosis

Protein Pumps

What is the primary focus of Lecture 6?

Cell Culture Media

Growing Mammalian Cells

Nutrient Uptake (correct)

Contamination Control

What is NOT a mechanism of active transport?

Facilitated Diffusion (correct)

Protein Pumps

Endocytosis

Osmosis (correct)

In which week is the recap of Lectures 2 through 5 scheduled?

Week 4 Signup and view all the answers

Which lecture covers the biology of culture cells?

Lecture 8 Signup and view all the answers

What is the primary function of a carrier protein in facilitated diffusion?

To bind solute and change shape Signup and view all the answers

What essential knowledge is necessary before manipulating cells for product production?

Understanding nutritional requirements Signup and view all the answers

Which statement correctly describes channel proteins?

They have a hydrophilic pore for rapid transport. Signup and view all the answers

What type of immune response is covered in Lecture 15?

Innate immune response Signup and view all the answers

What is the primary function of the transport protein mentioned?

To bind sodium ions and a phosphate group Signup and view all the answers

What happens to the protein channel after sodium ions are released to the outside of the membrane?

It reverts to its original shape after releasing potassium ions. Signup and view all the answers

Which process is an example of active transport?

Endocytosis Signup and view all the answers

What best defines active transport?

Transport that requires metabolic energy Signup and view all the answers

What direction do solutes move during facilitated diffusion?

Down their concentration gradient Signup and view all the answers

Which type of transport is characterized by the movement against the concentration gradient?

Active transport Signup and view all the answers

Which of the following does NOT characterize passive transport?

It occurs against the concentration gradient. Signup and view all the answers

What is the main purpose of endocytosis in a cell?

To bring large molecules into the cell Signup and view all the answers

Which of the following is NOT a type of endocytosis?

Exocytosis Signup and view all the answers

What feature distinguishes channel proteins from carrier proteins?

Channel proteins allow rapid movement through an aqueous channel. Signup and view all the answers

During which process do white blood cells consume bacteria?

Phagocytosis Signup and view all the answers

In active transport, what must occur to move molecules against their concentration gradient?

Metabolic energy is required. Signup and view all the answers

What initiates the change in shape of the transport protein channel?

Splitting of ATP Signup and view all the answers

Regarding facilitated diffusion, which statement is incorrect?

Energy is required for facilitated diffusion. Signup and view all the answers

Which statement regarding passive diffusion is true?

It does not have specificity for molecules transported. Signup and view all the answers

What is the process called when specific receptors on the cell surface bind to macromolecules for internalization?

Receptor-mediated Endocytosis Signup and view all the answers

What is the fate of the receptor after receptor-mediated endocytosis?

It is recycled back to the plasma membrane. Signup and view all the answers

Which of the following is NOT a type of transport mechanism for nutrients into the cell?

Absorption Signup and view all the answers

What distinguishes active transport from passive transport?

Active transport requires energy input. Signup and view all the answers

What occurs during exocytosis?

Material is forced out of the cell in bulk. Signup and view all the answers

Study Notes

Week 1-12 Lecture Schedule

Week 1 (Tue 10th Sept): Introduction to lecture modules
Week 2 (Mon 16th Sept): Use of mammalian cells, Lab layout and Equipment, Cell culture materials
Week 3 (Mon 23rd Sept): Contamination control
Week 4 (Mon 30th Sept): Recap of lectures 2, 3, and 4, assessment questions
Week 5 (Mon 07th Oct): Nutrient uptake
Week 6 (Mon 14th Oct): Biology of Culture Cells
Week 7 (Mon 21st Oct): Cell culture media, lab data analysis
Week 8 (Mon 04th Nov): Cell Culture Media, recap lectures 8-11 and assessment questions
Week 9 (Mon 11th Nov): Growing mammalian cells
Week 10 (Mon 18th Nov): Monitoring cell growth, cryopreservation cells; recap of lectures 12-14 and assessment question
Week 11 (Mon 25th Nov): Innate immune response, adaptive immune response and Bioassays
Week 12 (Mon 02nd Dec): Recap of lectures 15-16, assessment questions, Revision (Tue 03rd Dec)

Lecture 7: Nutrients - Membrane Transport

Lecture Overview (Slide 2): Introduction, nutrient uptake mechanism, conclusion
Introduction (Slide 3): Cell manipulation for desired products and services requires understanding nutritional requirements.
Passive Transport (Slide 4): Cell doesn't use energy (Diffusion, Osmosis, Facilitated Diffusion)
Active Transport (Slide 4): Cell uses energy (Protein Pumps, Endocytosis, Exocytosis)
Facilitated Diffusion (Slide 5): Transport of molecules down concentration gradient, facilitated by transport proteins (No energy input)
Facilitated Diffusion - Carrier Protein (Slide 6): Protein alternates conformations, moves solute across membrane, direction down concentration gradient
Facilitated Diffusion - Channel Protein (Slide 7): Aqueous channel, hydrophilic pore, rapid selective solute movement (size/charge).
Active Transport (Slide 8): Movement against concentration gradient, requires metabolic energy and transport proteins
Sodium-Potassium Pump (Slide 9): Specific receptors for sodium ions, phosphate group from ATP, shape change, release to outside, potassium ions bind, phosphate release, original shape, and potassium release back into the cell
Passive vs. Facilitated vs. Active Transport Comparison (Slide 10): Characteristics comparison (carrier proteins, transport speed, against concentration gradient, specificity for molecules)
Endocytosis and Exocytosis (Slide 11): Mechanism for large molecules, food, and waste into/out of cell. Endocytosis brings materials into the cell. Exocytosis moves materials out of the cell.
Endocytosis Details (Slide 12): Process to move substances into the cell without crossing the membrane. Types include phagocytosis (large particles), pinocytosis (small particles), and receptor-mediated endocytosis
Receptor-mediated Endocytosis (Slide 13): Specific receptor on the cell surface binds tightly to macromolecule, then undergoes endocytosis (receptor recycling) examples include cholesterol, transferrin, and insulin.
Exocytosis (Slide 14): Bulk material removal from the cell. Cell changes shape - requires energy. Examples include hormones and wastes
Conclusion (Slide 15): Nutrients enter the cell via passive or active transport mechanisms. Passive transport doesn't require energy, and active transport always requires energy.

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Description

This quiz covers key concepts from lectures on cell culture techniques including mammalian cells, nutrient uptake, contamination control, and immune responses. Review the essential processes and terminology related to cell culture and laboratory practices from weeks 1 to 12. Ideal for students seeking to reinforce their understanding of cell biology.