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Questions and Answers
Which section of the course will discuss how Recombinant DNA is inserted into a cell to create recombinant proteins?
Which section of the course will discuss how Recombinant DNA is inserted into a cell to create recombinant proteins?
What is the function of the first section of this course?
What is the function of the first section of this course?
What is DNA?
What is DNA?
What is the purpose of the second section of this course?
What is the purpose of the second section of this course?
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Which molecule is the building block of DNA?
Which molecule is the building block of DNA?
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What are the four bases found in DNA?
What are the four bases found in DNA?
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What is the process called when DNA is transcribed to RNA?
What is the process called when DNA is transcribed to RNA?
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What type of proteins require post-translational modifications to be fully functional?
What type of proteins require post-translational modifications to be fully functional?
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Which type of cells are more sensitive to changes in the cell culture environment such as temperature and pH?
Which type of cells are more sensitive to changes in the cell culture environment such as temperature and pH?
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What is the main limitation of using bacterial cells to create proteins?
What is the main limitation of using bacterial cells to create proteins?
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Which recombinant protein is used to replace a missing protein in patients with Hemophilia A?
Which recombinant protein is used to replace a missing protein in patients with Hemophilia A?
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What is the main function of Herceptin in treating breast cancer?
What is the main function of Herceptin in treating breast cancer?
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Which of the following best describes recombinant DNA?
Which of the following best describes recombinant DNA?
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What is the role of restriction enzymes in genetic engineering?
What is the role of restriction enzymes in genetic engineering?
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What is a plasmid?
What is a plasmid?
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How is the gene of interest integrated into a plasmid?
How is the gene of interest integrated into a plasmid?
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Which enzyme is used to create a gap in the plasmid during the process of making a recombinant plasmid?
Which enzyme is used to create a gap in the plasmid during the process of making a recombinant plasmid?
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What is the purpose of using bacterial cells to create recombinant proteins?
What is the purpose of using bacterial cells to create recombinant proteins?
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What are the two common methods used to transfer a plasmid into bacterial cells?
What are the two common methods used to transfer a plasmid into bacterial cells?
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What is the function of DNA ligase in the process of making a recombinant plasmid?
What is the function of DNA ligase in the process of making a recombinant plasmid?
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Which method is used to get rid of bacterial cells that do not contain the plasmid?
Which method is used to get rid of bacterial cells that do not contain the plasmid?
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What is the reason for using a liposome in the process of transferring recombinant DNA into mammalian cells?
What is the reason for using a liposome in the process of transferring recombinant DNA into mammalian cells?
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Why can't antibiotic selection be used to select only the mammalian cells that have taken up the plasmid?
Why can't antibiotic selection be used to select only the mammalian cells that have taken up the plasmid?
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What is one advantage of using bacterial cells over mammalian cells for creating recombinant proteins?
What is one advantage of using bacterial cells over mammalian cells for creating recombinant proteins?
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Which of the following best describes fusion proteins?
Which of the following best describes fusion proteins?
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What are Alprolix and Eloctate?
What are Alprolix and Eloctate?
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Why are fusion proteins like Alprolix and Eloctate important for hemophiliacs?
Why are fusion proteins like Alprolix and Eloctate important for hemophiliacs?
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Why are antibodies considered stable in a patient's body?
Why are antibodies considered stable in a patient's body?
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What is the advantage of fusing the gene for a blood clotting factor with a portion of the antibody gene?
What is the advantage of fusing the gene for a blood clotting factor with a portion of the antibody gene?
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What are genetically engineered animals used for in the production of biologic drugs?
What are genetically engineered animals used for in the production of biologic drugs?
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