Lecture 15
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Questions and Answers

What is the capital of France? (example question)

  • Paris (correct)
  • London
  • Berlin
  • Madrid
  • What is the main characteristic of bacterial mRNAs?

  • They are monocistronic.
  • They are polycistronic. (correct)
  • They are circular.
  • They contain introns.
  • Which site on the ribosome is responsible for the exit of tRNA?

  • A site
  • P site
  • E site (correct)
  • S site
  • What sequence on mRNA helps in ribosome binding during bacterial translation?

    <p>Shine-Dalgarno sequence</p> Signup and view all the answers

    Which ribosomal subunit contains the 23S rRNA?

    <p>50S</p> Signup and view all the answers

    What role does EF-Tu play during bacterial translation?

    <p>It guides the next aminoacyl-tRNA into the A site.</p> Signup and view all the answers

    What is required for the formation of peptide bonds during translation?

    <p>Peptidyl transferase activity</p> Signup and view all the answers

    In eukaryotic translation, which structure helps stabilize the mRNA?

    Signup and view all the answers

    What type of polymerization occurs during amino acid chain elongation?

    <p>Head polymerization</p> Signup and view all the answers

    What is the function of the Kozak sequence in eukaryotic mRNA?

    <p>It facilitates ribosome binding and identifies the start codon.</p> Signup and view all the answers

    Which factor is responsible for the scanning process in eukaryotic translation?

    <p>eIF4A</p> Signup and view all the answers

    What does eEF2 do during elongation in eukaryotic translation?

    Signup and view all the answers

    Which of the following is a difference between prokaryotic and eukaryotic ribosomes?

    <p>Prokaryotic ribosomes have a 70S structure.</p> Signup and view all the answers

    What is the role of the release factor in eukaryotic translation?

    <p>It recognizes stop codons and facilitates termination.</p> Signup and view all the answers

    What is the primary function of ribosomes during translation?

    <p>To catalyze the formation of peptide bonds.</p> Signup and view all the answers

    What does the acronym "eRF" stand for in the context of translation?

    <p>Eukaryotic Release Factor</p> Signup and view all the answers

    What does puromycin mimic in the process of translation?

    <p>tRNA</p> Signup and view all the answers

    Which ribosomal site holds the tRNA with the growing polypeptide chain?

    <p>P site</p> Signup and view all the answers

    What happens to the ribosome after termination of translation?

    <p>It disassociates into its subunits.</p> Signup and view all the answers

    Which of the following is a consequence of ricin action on the ribosome?

    <p>Inhibition of translocation</p> Signup and view all the answers

    What is the main evolutionary significance of eukaryotic translation machinery?

    <p>It indicates a common ancestor with bacteria.</p> Signup and view all the answers

    What is a major difference in the structure of eukaryotic versus prokaryotic mRNA?

    <p>Eukaryotic mRNA has a longer 5' UTR.</p> Signup and view all the answers

    Which type of ribosomal RNA is involved in the peptidyl transferase activity?

    <p>23S rRNA</p> Signup and view all the answers

    What is the primary role of the initiation factors in eukaryotic translation?

    <p>To coordinate the formation of the initiation complex.</p> Signup and view all the answers

    What is the main reason for the circularization of eukaryotic mRNA?

    <p>To facilitate efficient translation.</p> Signup and view all the answers

    Which of the following best describes the role of EF-G in bacterial translation?

    <p>It promotes ribosome translocation.</p> Signup and view all the answers

    What type of bacterial ribosome is formed by the association of the 30S and 50S subunits?

    <p>70S</p> Signup and view all the answers

    What is the main function of the Shine-Dalgarno sequence?

    <p>To recruit the ribosome to the mRNA.</p> Signup and view all the answers

    What is the primary action of the release factor during translation termination?

    <p>It hydrolyzes the bond between the polypeptide and tRNA.</p> Signup and view all the answers

    Which evolutionary event likely led to the development of eukaryotic translation machinery?

    <p>The fusion of archaea and bacteria.</p> Signup and view all the answers

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