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Light-Dependent Stage of Photosynthesis
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Light-Dependent Stage of Photosynthesis

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Questions and Answers

What occurs during the light-dependent stage of photosynthesis?

  • Carbon fixation
  • Splitting of water molecules (correct)
  • Production of ribulose bisphosphate
  • Synthesis of glucose
  • Which complex is responsible for the photolysis of water?

  • Photosystem I
  • Electron transport chain
  • Photosystem II (correct)
  • ATP synthase
  • What is the outcome of the reaction 2H2O → 4H+ + 4e− + O2?

  • Formation of ATP
  • Production of glucose
  • Release of oxygen (correct)
  • Reduction of NADP
  • Which process describes how ATP is produced during the light-dependent reactions?

    <p>Photophosphorylation</p> Signup and view all the answers

    What role do the electrons play once they are released from chlorophyll in PSII?

    <p>They are picked up by electron carriers.</p> Signup and view all the answers

    What happens to the protons (H+) after they are released from water during photolysis?

    <p>They build up in the thylakoid space.</p> Signup and view all the answers

    Where do the electrons emitted from PSII ultimately end up?

    <p>In NADP</p> Signup and view all the answers

    How are protons allowed to move back into the stroma from the thylakoid space?

    <p>Facilitated diffusion</p> Signup and view all the answers

    Which component primarily absorbs light energy during the light-dependent reactions?

    <p>Chlorophyll</p> Signup and view all the answers

    What is the final product of the combination of electrons from PSI and protons from photolysis?

    <p>NADPH</p> Signup and view all the answers

    What is the primary function of the chlorophyll in PSII during the photosynthesis process?

    <p>To raise the energy of electrons upon light absorption.</p> Signup and view all the answers

    What are the end products of the light-dependent stage of photosynthesis?

    <p>ATP and reduced NADP.</p> Signup and view all the answers

    What occurs during the Calvin cycle in the light-independent stage?

    <p>Glycerate 3-phosphate is produced from ribulose bisphosphate.</p> Signup and view all the answers

    What is the role of ATP in the Calvin cycle?

    <p>It is used to regenerate ribulose bisphosphate.</p> Signup and view all the answers

    Which of the following describes cyclic photophosphorylation?

    <p>It exclusively generates ATP without producing reduced NADP.</p> Signup and view all the answers

    What is the result of combining carbon dioxide with ribulose bisphosphate (RuBP)?

    <p>It produces two molecules of glycerate 3-phosphate (GP).</p> Signup and view all the answers

    What does triose phosphate (TP) primarily get used for in photosynthesis?

    <p>To regenerate ribulose bisphosphate.</p> Signup and view all the answers

    Which of the following substances is produced in the light-independent reactions?

    <p>Glucose.</p> Signup and view all the answers

    During which part of photosynthesis does the enzyme rubisco function?

    <p>In the Calvin cycle within the stroma.</p> Signup and view all the answers

    What is a primary role of reduced NADP in photosynthesis?

    <p>To provide hydrogen for the reduction of glycerate 3-phosphate.</p> Signup and view all the answers

    Study Notes

    Light-Dependent Stage

    • Occurs only in the presence of light
    • Energy from light is used to split water molecules and produce ATP and reduced NADP
    • Two pathways: non-cyclic photophosphorylation and cyclic photophosphorylation
    • Non-cyclic photophosphorylation
      • Chlorophyll molecules in PSI and PSII absorb light energy
      • Energy excites electrons, raising their energy level
        • Chlorophyll is said to be photoactivated
      • PSII contains an enzyme in the oxygen-evolving complex that splits water when activated by light
        • Photolysis: splitting by light
      • Water molecules are split into oxygen and hydrogen atoms
      • Each hydrogen atom loses its electron to become a positively charged hydrogen ion (proton), H+
      • Electrons from the hydrogen replace the electrons lost from chlorophyll in PSII
      • Oxygen atoms from the water combine to form oxygen molecules, which diffuse out of the chloroplast and into the air around the leaf
        • Equation for the reaction: 2H2O light 4H+ + 4e− + O2
      • Electrons emitted from PSII are picked up by electron carriers (electron transport chain) in the membranes of the thylakoids
      • As the electrons move along the chain they release energy
        • Energy is used to actively transport protons across the thylakoid membrane into the space between the membranes
        • High concentration of protons build up in the thylakoid space
        • Protons move back into the stroma from the thylakoid space by facilitated diffusion through ATP synthases, by chemiosmosis.
        • This process provides energy to cause ADP and inorganic phosphate to combine to make ATP (photophosphorylation)
      • At the end of the electron carrier chain, electrons are picked up by PSI
      • Electrons from PSI are passed along a different chain of carriers to NADP
      • NADP also picks up protons (hydrogen ions) from the split water molecules
        • NADP becomes reduced NADP
    • Z- scheme
      • Diagram that illustrates the light-dependant stage
      • Shows the energy levels of electrons
      • Follows the journey of an electron from a water molecule
        • Taken up by PSII
        • Energy is raised as chlorophyll in PSII absorbs light energy
        • Loses some energy as it passes along the electron carrier chain
        • Taken up by PSI
        • Energy is raised again as chlorophyll in PSI absorbs light energy
        • Becomes part of a reduced NADP molecule
    • Cyclic photophosphorylation
      • Alternative pathway for the electron emitted from PSI
      • Electron is passed along the electron transport chain, then back to PSI
      • ATP is produced through photophosphorylation
      • No reduced NADP is produced

    Light-Independent Stage

    • Series of reactions that can take place even without light
    • Uses ATP and NADP from the light-dependent stage to synthesize carbohydrates from carbon dioxide
    • Known as the Calvin cycle
    • Takes place in the stroma of the chloroplast
    • Rubisco
      • Enzyme found in the stroma
      • Carbon dioxide diffuses into the stroma from the air spaces within the leaf
      • Carbon dioxide enters the active site of rubisco, combining with ribulose bisphosphate (RuBP)
      • Two 3C molecules of glycerate 3-phosphate (GP) are produced
      • Carbon fixation: combination of carbon dioxide with RuBP
    • Energy from ATP and hydrogen from reduced NADP are used to reduce GP to triose phosphate (TP)
      • TP is the first carbohydrate produced in photosynthesis
      • Most of TP is used to regenerate RuBP, allowing more Carbon dioxide to be fixed
      • This process uses ATP
    • Remaining TP is used to produce glucose or other organic substances the plant cell requires
      • Includes polysaccharides (starch for energy storage, cellulose for making cell walls), sucrose for transport, amino acids for making proteins, lipids for energy storage, and nucleotides for making DNA and RNA
      • Some of the GP is directly used to produce certain amino acids

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    Description

    Explore the light-dependent stage of photosynthesis where light energy is utilized to split water molecules and generate ATP and reduced NADP. This quiz covers the two pathways: non-cyclic and cyclic photophosphorylation, including the role of chlorophyll and the process of photolysis.

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