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Questions and Answers
What is the typical capacity range of large volume preparative protocols?
What is the typical capacity range of large volume preparative protocols?
- 100-500 mL
- 300-1700 mL (correct)
- 1000-3000 mL
- 5000-7000 mL
Why are certain protocols habitually chosen?
Why are certain protocols habitually chosen?
- For experimental protocols
- For analytical protocols
- For small volume preparative protocols
- For large volume preparative protocols (correct)
What is the lower limit of the typical capacity of large volume preparative protocols?
What is the lower limit of the typical capacity of large volume preparative protocols?
- 300 mL (correct)
- 200 mL
- 400 mL
- 100 mL
What is the upper limit of the typical capacity of large volume preparative protocols?
What is the upper limit of the typical capacity of large volume preparative protocols?
What type of protocols are being discussed?
What type of protocols are being discussed?
What is the angle measured with respect to?
What is the angle measured with respect to?
What is the result of a shorter sedimentation pathlength?
What is the result of a shorter sedimentation pathlength?
What is the angle of a rotor mentioned in the text?
What is the angle of a rotor mentioned in the text?
What is the relationship between the angle of the rotor and the sedimentation pathlength?
What is the relationship between the angle of the rotor and the sedimentation pathlength?
What is the characteristic of a rotor with a fixed angle?
What is the characteristic of a rotor with a fixed angle?
What is the purpose of the hole in the bottom of the centrifugal tube?
What is the purpose of the hole in the bottom of the centrifugal tube?
What is the direction of the gradient displacement?
What is the direction of the gradient displacement?
How are the fractions collected from the tube?
How are the fractions collected from the tube?
What happens to the particles of different densities during centrifugation?
What happens to the particles of different densities during centrifugation?
What is the result of using a more dense solution in the centrifugal tube?
What is the result of using a more dense solution in the centrifugal tube?
Which type of viruses are related to human diseases?
Which type of viruses are related to human diseases?
What is an example of a human disease caused by a retrovirus?
What is an example of a human disease caused by a retrovirus?
Which of the following diseases is NOT caused by a retrovirus?
Which of the following diseases is NOT caused by a retrovirus?
What is another name for a type of virus that is related to human diseases?
What is another name for a type of virus that is related to human diseases?
How many types of viruses are related to human diseases?
How many types of viruses are related to human diseases?
What is the duration of the process of suspending the pellet and mixing it with a concentrated salt solution?
What is the duration of the process of suspending the pellet and mixing it with a concentrated salt solution?
What is the final density of the mixture after resuspending the pellet?
What is the final density of the mixture after resuspending the pellet?
What is the amount of the pellet in grams?
What is the amount of the pellet in grams?
What is the purpose of adding a concentrated salt solution to the pellet?
What is the purpose of adding a concentrated salt solution to the pellet?
What is the resulting layer obtained after the process?
What is the resulting layer obtained after the process?
Study Notes
Centrifugation Protocols
- Large volume preparative protocols typically have capacities ranging from 300-1700 mL.
- The sedimentation path length is shorter in a rotor with a fixed angle (e.g., 40°) compared to a vertical rotor.
Centrifugation Techniques
- To collect fractions, a hole is punctured in the bottom of the centrifugation tube, and the fractions are collected from top to bottom, from higher to lower density parts of the gradient.
- Displacement of the gradient upward is achieved using a more dense solution.
Characteristics of Retroviruses
- Several retroviruses are related to human diseases, such as influenza and HIV.
Density Gradient Centrifugation
- A floating layer of LDL is obtained, and the pellet is resuspended and adjusted to a final density of 1.21 g/mL.
- The process involves centrifugation at 100,000 g for 28 hours, followed by resuspension and adjustment of the density to 1.063 g/mL.
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Description
This quiz covers the use of large volume preparative chromatography in protocols, typically with capacities ranging from 300-1700 mL. It's a crucial step in many laboratory processes.
