5 Questions
What is the purpose of preparative chromatography?
To separate the components of a mixture in large scales for further use
In chromatography, what is the role of the 'mobile phase'?
To dissolve the mixture being separated
What causes the various constituents of a mixture to separate in chromatography?
Differential partitioning between the mobile and stationary phases
In solvent extraction, what happens when the sample is acidified?
Acidic components are less likely to be extracted
What is the main basis for the separation of compounds in chromatography?
Differences in a compound's partition coefficient
Study Notes
Preparative Chromatography
- The purpose of preparative chromatography is to prepare or isolate specific compounds or substances from a mixture on a large scale.
Chromatography Basics
- The mobile phase in chromatography is the substance that carries the sample through the stationary phase, facilitating separation of the mixture's constituents.
Separation Mechanism
- The constituents of a mixture separate in chromatography due to differences in their interactions with the stationary and mobile phases, causing them to migrate at varying rates.
Solvent Extraction
- When a sample is acidified in solvent extraction, the pH change can affect the distribution of compounds between the two solvents, enhancing the extraction of target compounds.
Separation Principles
- The main basis for the separation of compounds in chromatography is the varying degrees of attraction between the constituents and the stationary and mobile phases.
Test your knowledge of laboratory techniques used for the separation of mixtures with this chromatography quiz.
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