Chromatography Techniques
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Questions and Answers

What is the purpose of preparative chromatography?

  • To extract acidic and basic components from a mixture
  • To analyze the components of a mixture
  • To separate the components of a mixture in large scales for further use (correct)
  • To purify a mixture using solvent extraction
  • In chromatography, what is the role of the 'mobile phase'?

  • To dissolve the mixture being separated (correct)
  • To cause differential retention on the stationary phase
  • To change the separation based on partition coefficient
  • To hold the stationary phase
  • What causes the various constituents of a mixture to separate in chromatography?

  • Differential partitioning between the mobile and stationary phases (correct)
  • The extraction selective towards acidic and basic components
  • The pH alteration of the aqueous layer
  • The differential retention on the stationary phase
  • In solvent extraction, what happens when the sample is acidified?

    <p>Acidic components are less likely to be extracted</p> Signup and view all the answers

    What is the main basis for the separation of compounds in chromatography?

    <p>Differences in a compound's partition coefficient</p> Signup and view all the answers

    Study Notes

    Preparative Chromatography

    • The purpose of preparative chromatography is to prepare or isolate specific compounds or substances from a mixture on a large scale.

    Chromatography Basics

    • The mobile phase in chromatography is the substance that carries the sample through the stationary phase, facilitating separation of the mixture's constituents.

    Separation Mechanism

    • The constituents of a mixture separate in chromatography due to differences in their interactions with the stationary and mobile phases, causing them to migrate at varying rates.

    Solvent Extraction

    • When a sample is acidified in solvent extraction, the pH change can affect the distribution of compounds between the two solvents, enhancing the extraction of target compounds.

    Separation Principles

    • The main basis for the separation of compounds in chromatography is the varying degrees of attraction between the constituents and the stationary and mobile phases.

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    Description

    Test your knowledge of laboratory techniques used for the separation of mixtures with this chromatography quiz.

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