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Questions and Answers
What is the purpose of preparative chromatography?
What is the purpose of preparative chromatography?
- To extract acidic and basic components from a mixture
- To analyze the components of a mixture
- To separate the components of a mixture in large scales for further use (correct)
- To purify a mixture using solvent extraction
In chromatography, what is the role of the 'mobile phase'?
In chromatography, what is the role of the 'mobile phase'?
- To dissolve the mixture being separated (correct)
- To cause differential retention on the stationary phase
- To change the separation based on partition coefficient
- To hold the stationary phase
What causes the various constituents of a mixture to separate in chromatography?
What causes the various constituents of a mixture to separate in chromatography?
- Differential partitioning between the mobile and stationary phases (correct)
- The extraction selective towards acidic and basic components
- The pH alteration of the aqueous layer
- The differential retention on the stationary phase
In solvent extraction, what happens when the sample is acidified?
In solvent extraction, what happens when the sample is acidified?
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What is the main basis for the separation of compounds in chromatography?
What is the main basis for the separation of compounds in chromatography?
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Study Notes
Preparative Chromatography
- The purpose of preparative chromatography is to prepare or isolate specific compounds or substances from a mixture on a large scale.
Chromatography Basics
- The mobile phase in chromatography is the substance that carries the sample through the stationary phase, facilitating separation of the mixture's constituents.
Separation Mechanism
- The constituents of a mixture separate in chromatography due to differences in their interactions with the stationary and mobile phases, causing them to migrate at varying rates.
Solvent Extraction
- When a sample is acidified in solvent extraction, the pH change can affect the distribution of compounds between the two solvents, enhancing the extraction of target compounds.
Separation Principles
- The main basis for the separation of compounds in chromatography is the varying degrees of attraction between the constituents and the stationary and mobile phases.
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