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Questions and Answers
What is the purpose of preparative chromatography?
What is the purpose of preparative chromatography?
In chromatography, what is the role of the 'mobile phase'?
In chromatography, what is the role of the 'mobile phase'?
What causes the various constituents of a mixture to separate in chromatography?
What causes the various constituents of a mixture to separate in chromatography?
In solvent extraction, what happens when the sample is acidified?
In solvent extraction, what happens when the sample is acidified?
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What is the main basis for the separation of compounds in chromatography?
What is the main basis for the separation of compounds in chromatography?
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Study Notes
Preparative Chromatography
- The purpose of preparative chromatography is to prepare or isolate specific compounds or substances from a mixture on a large scale.
Chromatography Basics
- The mobile phase in chromatography is the substance that carries the sample through the stationary phase, facilitating separation of the mixture's constituents.
Separation Mechanism
- The constituents of a mixture separate in chromatography due to differences in their interactions with the stationary and mobile phases, causing them to migrate at varying rates.
Solvent Extraction
- When a sample is acidified in solvent extraction, the pH change can affect the distribution of compounds between the two solvents, enhancing the extraction of target compounds.
Separation Principles
- The main basis for the separation of compounds in chromatography is the varying degrees of attraction between the constituents and the stationary and mobile phases.
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Description
Test your knowledge of laboratory techniques used for the separation of mixtures with this chromatography quiz.