Questions and Answers
What must be done with contaminated materials intended for incineration?
How often should thermometers be checked for gas bubbles to ensure accuracy?
What is the required frequency for checking the temperature of a biofreezer?
What should be done with thermometers that differ by more than 1°C from the reference thermometer?
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How often must the face velocity and operation of a fume hood be tested?
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In what circumstance should halogenated hydrocarbon extinguishers be recommended?
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What is the monitoring frequency for checking the rpm of a centrifuge?
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Where should acid and base spill kits be kept?
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What is the recommended action when faced with burning liquids?
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What is the frequency for checking the piston of a pipet?
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Study Notes
Laboratory Waste Recommended Treatment
- Incinerate onsite contaminated materials or store safely before transportation.
- Decontaminate liquid waste, including potentially contaminated liquids, before disposal in the sanitary sewer.
Equipment Quality Control
- Thermometers must be checked against a reference thermometer from NIST periodically.
- Discard thermometers deviating by more than 1°C from the reference.
- Daily inspection for gas bubbles in thermometers is essential for accurate readings.
- Centrifuge speed (rpm) should be checked biannually using a tachometer.
Quality Control of Instruments and Equipment
- Analytical balances: accuracy checked at least once a year.
- Biofreezer and refrigerator temperatures monitored daily.
- Biohazard hoods’ airflow confirmed at least once a year or after relocation.
- Pipets to be checked every 3 to 6 months for piston accuracy.
- Water baths and heating blocks require daily temperature checks.
- Fume hood performance evaluated annually for face velocity and operation.
Safety Protocols
- Display safety signage and infographics in laboratories.
- Maintain acid, base, and flammable spill kits near relevant usage areas.
- Halogenated hydrocarbon extinguishers recommended for use around computer equipment.
- Water should never be used to extinguish burning liquids or electrical equipment fires.
- Most common injuries in labs involve eyes, mouth, respiratory, and digestive tracts.
- Do not wash and reuse gloves to avoid difficulty in removing adhering microorganisms.
Best Practices for Specimen Handling
- Keep specimens capped during centrifugation to prevent aerosol formation, minimizing infection risks.
- Capping and uncapping specimens introduces biohazard risks due to aerosol fluids.
Occupational Safety and Health Administration (OSHA) Guidelines
- The hepatitis B vaccine must be offered free of charge to personnel at risk of exposure to bloodborne pathogens.
- Prohibit eating, drinking, or applying cosmetics within laboratory environments to maintain safety.
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Description
This quiz covers the recommended treatment for laboratory waste as per the WHO Laboratory Biosafety Manual, 2020. It details the incineration requirements for contaminated materials and the decontamination processes for liquid waste prior to disposal. Test your knowledge on proper laboratory waste management practices.
