Podcast
Questions and Answers
What is the type of cancer that Burkitt lymphoma is a part of?
What is the type of cancer that Burkitt lymphoma is a part of?
- Non-Hodgkin lymphoma (correct)
- Melanoma
- Leukemia
- Hodgkin lymphoma
In which region of the world does endemic Burkitt lymphoma most often develop in children?
In which region of the world does endemic Burkitt lymphoma most often develop in children?
- South America
- Africa (correct)
- Asia
- Europe
What is the purpose of a biopsy in diagnosis of Burkitt lymphoma?
What is the purpose of a biopsy in diagnosis of Burkitt lymphoma?
- To determine the severity of the disease
- To identify the genetic changes in the cells
- To rule out Burkitt lymphoma
- To confirm the presence of Burkitt lymphoma (correct)
What is the name of the gene translocation that is assessed in molecular diagnosis of Burkitt lymphoma?
What is the name of the gene translocation that is assessed in molecular diagnosis of Burkitt lymphoma?
What is the term for the study of the gross genomic changes of lymphoma cells?
What is the term for the study of the gross genomic changes of lymphoma cells?
Which of the following groups of people are at risk of developing immunodeficiency-related Burkitt lymphoma?
Which of the following groups of people are at risk of developing immunodeficiency-related Burkitt lymphoma?
What is the advantage of using karyotyping in genetic diagnosis?
What is the advantage of using karyotyping in genetic diagnosis?
What is the purpose of using TRIzolâ„¢ reagent in RNA isolation?
What is the purpose of using TRIzolâ„¢ reagent in RNA isolation?
What is the function of SYBR®Green I dye in the GoTaq®1-Step RT-qPCR System kit?
What is the function of SYBR®Green I dye in the GoTaq®1-Step RT-qPCR System kit?
What is the purpose of the GoTaq®1-Step RT-qPCR System kit?
What is the purpose of the GoTaq®1-Step RT-qPCR System kit?
What is the recommended range of A260/280 readings for ensuring RNA purity?
What is the recommended range of A260/280 readings for ensuring RNA purity?
What is the purpose of the GoScriptâ„¢ RT Mix in the 1-Step RT-qPCR System?
What is the purpose of the GoScriptâ„¢ RT Mix in the 1-Step RT-qPCR System?
What is the purpose of c-MYC gene expression analysis in clinical diagnosis?
What is the purpose of c-MYC gene expression analysis in clinical diagnosis?
What is the concentration of the forward primer in the reaction mixture?
What is the concentration of the forward primer in the reaction mixture?
How many cycles of denaturation are performed in the real-time PCR reaction?
How many cycles of denaturation are performed in the real-time PCR reaction?
What is the temperature of the extension step in the real-time PCR reaction?
What is the temperature of the extension step in the real-time PCR reaction?
What is the purpose of the β-actin gene in the reaction?
What is the purpose of the β-actin gene in the reaction?
What is the total volume of the reaction mixture?
What is the total volume of the reaction mixture?
Flashcards
Burkitt Lymphoma
Burkitt Lymphoma
A type of cancer that is a subtype of Non-Hodgkin lymphoma.
Endemic Burkitt Lymphoma
Endemic Burkitt Lymphoma
This lymphoma often develops in children in Africa.
Biopsy in Burkitt Lymphoma Diagnosis
Biopsy in Burkitt Lymphoma Diagnosis
A procedure to confirm the presence of Burkitt lymphoma by examining tissue samples.
c-MYC Translocation
c-MYC Translocation
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Karyotyping
Karyotyping
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At-risk Group for Immunodeficiency-related Burkitt Lymphoma
At-risk Group for Immunodeficiency-related Burkitt Lymphoma
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Advantage of Karyotyping
Advantage of Karyotyping
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Purpose of TRIzolâ„¢ Reagent
Purpose of TRIzolâ„¢ Reagent
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Function of SYBR®Green I Dye
Function of SYBR®Green I Dye
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Purpose of GoTaq® 1-Step RT-qPCR System
Purpose of GoTaq® 1-Step RT-qPCR System
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Recommended A260/280 Range
Recommended A260/280 Range
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Purpose of GoScriptâ„¢ RT Mix
Purpose of GoScriptâ„¢ RT Mix
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c-MYC Gene Expression Analysis
c-MYC Gene Expression Analysis
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Forward Primer Concentration
Forward Primer Concentration
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Denaturation Cycles
Denaturation Cycles
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Extension Step Temperature
Extension Step Temperature
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Purpose of β-actin Gene
Purpose of β-actin Gene
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Total Reaction Volume
Total Reaction Volume
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Study Notes
Burkitt Lymphoma
- A fast-growing cancer and a type of non-Hodgkin lymphoma (NHL)
- Characterized by many genetic changes that cause cells to act differently than normal cells
- Three types:
- Endemic Burkitt lymphoma: most often develops in children living in Africa
- Sporadic Burkitt lymphoma: affects children worldwide and is very rare in adults
- Immunodeficiency-related Burkitt lymphoma: develops in people with immune system problems
Diagnosis of Burkitt Lymphoma
- Biopsy: examining a sample of tissue under a microscope to confirm or rule out Burkitt lymphoma
- Molecular diagnosis:
- Cytogenetic findings: assessing gross genomic changes of lymphoma cells, including ploidy, deletions, translocations, and marker chromosomes
- Karyotyping: identifying the partner chromosome of the c-MYC translocation
- Gene expression: analyzing molecular alterations leading to c-MYC dysregulation and its effect on prognosis and diagnosis
Molecular Diagnosis of c-MYC Gene Translocation
- RNA isolation: using TRIzolâ„¢ reagent to isolate total RNA from patients and healthy controls
- RNA quantification: using nanodrop A260/280 readings to ensure purity
- Reverse Transcription-Quantitative PCR (RT-qPCR): using the GoTaq® 1-Step RT-qPCR System kit to analyze RNA
- Synthesis of complementary DNA (cDNA) from mRNA: using GoScriptâ„¢ RT Mix for 1-Step RT-qPCR
- Primer sequences:
- c-myc forward primer: 5′-TAC CCT CTC AAC GAC AGC AG-3′
- c-myc reverse primer: 5′-TCT TGA CAT TCT CCT CGG TG-3′
- β-actin forward primer: 5′-TCA CCC ACA CTG TGC CCA TCT ACG A-3′
- β-actin reverse primer: 5′CAG CGG AAC CGC TCA TTG CCA ATG G-3′ (control gene)
Real-Time PCR Reaction
- Steps:
- cDNA Synthesis: 37°C for 15 minutes
- Initial Denaturation: 95°C for 5 minutes
- Denaturation: 95°C for 30 seconds
- Annealing: 60°C for 30 seconds
- Extension: 72°C for 30 seconds
- Melt curve: 65-90°C
- Holding: 4°C
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Description
This lab quiz covers the basics of genetic disease and molecular diagnosis, focusing on Burkitt lymphoma, a type of non-Hodgkin lymphoma. Learn about the three types of Burkitt lymphoma and the genetic changes that cause them. Test your knowledge of molecular diagnosis and its applications in genetic disease.
