Lab 1: Genetic Diseases and Molecular Diagnosis

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What is the primary basis for diagnosing a genetic condition?

Family history and physical characteristics (correct)

Types of equipment used in Genetic diseases and molecular diagnosis

Results of a screening test

PCR technique

Who developed the polymerase chain reaction (PCR) technique?

Nobel Prize winner

Dr. Hassan Lafta

American biochemist

Kary Mullis (correct)

What is the main purpose of PCR technique in the lab?

To diagnose a genetic disease

To make millions of copies of a particular section of DNA (correct)

To perform a comprehensive clinical examination

To analyze a person's physical characteristics

What was RT-PCR used for during the Covid-19 pandemic?

To confirm whether a person was infected with the virus Signup and view all the answers

What is the function of RT-PCR?

To transcribe a portion of the RNA genome into complementary DNA (cDNA) Signup and view all the answers

What is one of the types of equipment used in Genetic diseases and molecular diagnosis?

Nanodrop Signup and view all the answers

What is the typical volume of sample required for measurement using NanoDrop spectrophotometer?

1-2 µL Signup and view all the answers

What is the generally accepted ratio for 'pure' DNA?

~1.8 Signup and view all the answers

What is the purpose of Gel Electrophoresis?

To separate mixtures of DNA, RNA, or proteins according to size Signup and view all the answers

What percentage of agarose gel is often used for standard electrophoresis?

1% Signup and view all the answers

What is the purpose of Ethidium bromide?

To stain DNA or RNA and visualize with UV light Signup and view all the answers

What type of gel is suitable for separating small 0.2-1kb DNA fragments?

2% agarose gel Signup and view all the answers

What is the primary purpose of a centrifuge?

To separate substances of different densities Signup and view all the answers

What is the purpose of an electronic balance?

To accurately measure the weight of chemicals Signup and view all the answers

Lab 1: Genetic Diseases and Molecular Diagnosis

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Questions and Answers

What is the primary basis for diagnosing a genetic condition?

Who developed the polymerase chain reaction (PCR) technique?

What is the main purpose of PCR technique in the lab?

What was RT-PCR used for during the Covid-19 pandemic?

What is the function of RT-PCR?

What is one of the types of equipment used in Genetic diseases and molecular diagnosis?

What is the typical volume of sample required for measurement using NanoDrop spectrophotometer?

What is the generally accepted ratio for 'pure' DNA?

What is the purpose of Gel Electrophoresis?

What percentage of agarose gel is often used for standard electrophoresis?

What is the purpose of Ethidium bromide?

What type of gel is suitable for separating small 0.2-1kb DNA fragments?

What is the primary purpose of a centrifuge?

What is the purpose of an electronic balance?

What happens to inorganic compounds and large non-volatile organic molecules during distillation?

What should be visible after microwaving the media?

What is the result of distillation?

What is the purpose of heating contaminated water in distillation?

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