Lab 1: Genetic Diseases and Molecular Diagnosis
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Questions and Answers

What is the primary basis for diagnosing a genetic condition?

  • Family history and physical characteristics (correct)
  • Types of equipment used in Genetic diseases and molecular diagnosis
  • Results of a screening test
  • PCR technique

    • Who developed the polymerase chain reaction (PCR) technique?

  • Nobel Prize winner
  • Dr. Hassan Lafta
  • American biochemist
  • Kary Mullis (correct)

    • What is the main purpose of PCR technique in the lab?

  • To diagnose a genetic disease
  • To make millions of copies of a particular section of DNA (correct)
  • To perform a comprehensive clinical examination
  • To analyze a person's physical characteristics

    • What was RT-PCR used for during the Covid-19 pandemic?

    <p>To confirm whether a person was infected with the virus</p> Signup and view all the answers

    What is the function of RT-PCR?

    <p>To transcribe a portion of the RNA genome into complementary DNA (cDNA)</p> Signup and view all the answers

    What is one of the types of equipment used in Genetic diseases and molecular diagnosis?

    <p>Nanodrop</p> Signup and view all the answers

    What is the typical volume of sample required for measurement using NanoDrop spectrophotometer?

    <p>1-2 µL</p> Signup and view all the answers

    What is the generally accepted ratio for 'pure' DNA?

    <p>~1.8</p> Signup and view all the answers

    What is the purpose of Gel Electrophoresis?

    <p>To separate mixtures of DNA, RNA, or proteins according to size</p> Signup and view all the answers

    What percentage of agarose gel is often used for standard electrophoresis?

    <p>1%</p> Signup and view all the answers

    What is the purpose of Ethidium bromide?

    <p>To stain DNA or RNA and visualize with UV light</p> Signup and view all the answers

    What type of gel is suitable for separating small 0.2-1kb DNA fragments?

    <p>2% agarose gel</p> Signup and view all the answers

    What is the primary purpose of a centrifuge?

    <p>To separate substances of different densities</p> Signup and view all the answers

    What is the purpose of an electronic balance?

    <p>To accurately measure the weight of chemicals</p> Signup and view all the answers

    What happens to inorganic compounds and large non-volatile organic molecules during distillation?

    <p>They are left behind</p> Signup and view all the answers

    What should be visible after microwaving the media?

    <p>A clear solution with no granules</p> Signup and view all the answers

    What is the result of distillation?

    <p>Purified water</p> Signup and view all the answers

    What is the purpose of heating contaminated water in distillation?

    <p>To create steam</p> Signup and view all the answers

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