Lab 1: Genetic Diseases and Molecular Diagnosis

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Questions and Answers

What is the primary basis for diagnosing a genetic condition?

  • Family history and physical characteristics (correct)
  • Types of equipment used in Genetic diseases and molecular diagnosis
  • Results of a screening test
  • PCR technique

Who developed the polymerase chain reaction (PCR) technique?

  • Nobel Prize winner
  • Dr. Hassan Lafta
  • American biochemist
  • Kary Mullis (correct)

What is the main purpose of PCR technique in the lab?

  • To diagnose a genetic disease
  • To make millions of copies of a particular section of DNA (correct)
  • To perform a comprehensive clinical examination
  • To analyze a person's physical characteristics

What was RT-PCR used for during the Covid-19 pandemic?

<p>To confirm whether a person was infected with the virus (B)</p> Signup and view all the answers

What is the function of RT-PCR?

<p>To transcribe a portion of the RNA genome into complementary DNA (cDNA) (C)</p> Signup and view all the answers

What is one of the types of equipment used in Genetic diseases and molecular diagnosis?

<p>Nanodrop (D)</p> Signup and view all the answers

What is the typical volume of sample required for measurement using NanoDrop spectrophotometer?

<p>1-2 µL (B)</p> Signup and view all the answers

What is the generally accepted ratio for 'pure' DNA?

<p>~1.8 (A)</p> Signup and view all the answers

What is the purpose of Gel Electrophoresis?

<p>To separate mixtures of DNA, RNA, or proteins according to size (B)</p> Signup and view all the answers

What percentage of agarose gel is often used for standard electrophoresis?

<p>1% (C)</p> Signup and view all the answers

What is the purpose of Ethidium bromide?

<p>To stain DNA or RNA and visualize with UV light (C)</p> Signup and view all the answers

What type of gel is suitable for separating small 0.2-1kb DNA fragments?

<p>2% agarose gel (A)</p> Signup and view all the answers

What is the primary purpose of a centrifuge?

<p>To separate substances of different densities (A)</p> Signup and view all the answers

What is the purpose of an electronic balance?

<p>To accurately measure the weight of chemicals (D)</p> Signup and view all the answers

What happens to inorganic compounds and large non-volatile organic molecules during distillation?

<p>They are left behind (C)</p> Signup and view all the answers

What should be visible after microwaving the media?

<p>A clear solution with no granules (D)</p> Signup and view all the answers

What is the result of distillation?

<p>Purified water (C)</p> Signup and view all the answers

What is the purpose of heating contaminated water in distillation?

<p>To create steam (C)</p> Signup and view all the answers

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