Kjeldahl Method and Nitrogen Determination Quiz
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Questions and Answers

What does the Kjeldahl method not include in its measurement without modification?

  • Ammonia
  • Protein
  • Organic substances
  • Nitrate (correct)
  • What is added to make the digestion process go faster in the Kjeldahl method?

  • Water
  • Catalysts like selenium, Hg2SO4 or CuSO4 (correct)
  • Extra sulfuric acid
  • Additional organic substances
  • What is the Kjeldahl method used for?

  • Quantitative determination of all forms of nitrogen in a sample
  • Qualitative determination of protein content
  • Qualitative determination of nitrogen in inorganic compounds
  • Quantitative determination of nitrogen in organic substances and ammonia/ammonium (correct)
  • When is the digestion considered complete in the Kjeldahl method?

    <p>When the liquor clarifies with the release of fumes</p> Signup and view all the answers

    What is used as a known volume of standard acid at the end of the distillation system in Kjeldahl method?

    <p>Sulfuric acid (H2SO4)</p> Signup and view all the answers

    Study Notes

    Kjeldahl Method Overview

    • The Kjeldahl method does not measure nitrogen present in nitrates and nitrites without modification.
    • The method is primarily designed to quantify total nitrogen in organic compounds, excluding certain nitrogen forms.

    Acceleration of Digestion

    • To expedite the digestion process in the Kjeldahl method, catalysts such as selenium or copper are often added.

    Applications of the Kjeldahl Method

    • The Kjeldahl method is widely used for determining protein content in food, fertilizers, and feed, serving an essential role in nutritional analysis.

    Completion of Digestion

    • Digestion is considered complete when a clear solution is achieved, indicating that all organic nitrogen has been converted to ammonium ions.

    Standard Acid in Distillation

    • A known volume of hydrochloric acid (HCl) or sulfuric acid (H2SO4) is utilized as a standard acid at the end of the distillation system in the Kjeldahl method for titration purposes.

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    Description

    Test your knowledge of the Kjeldahl method, a chemical analysis technique used to quantitatively determine nitrogen in organic substances and inorganic compounds like ammonia and ammonium. Learn about its significance in measuring protein content and its limitations in detecting other forms of inorganic nitrogen.

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