Kinetic Studies of Biodegradation
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Questions and Answers

Which process converts aromatic hydrocarbons into intermediates such as catechol and protocatechuate?

  • Hydroxylation
  • Polymerization
  • Dehydrogenation
  • Biotransformation (correct)
  • What enzymatic activity is crucial for the co-metabolic degradation of organo-pollutants?

  • Decarboxylation reactions
  • Synthesis of cofactors (correct)
  • Hydrolysis of substrates
  • Nitrogen fixation
  • Which of the following microbial genera is known for its high degradation activity of aromatic compounds?

  • Rhodococcus (correct)
  • Bacillus
  • Clostridium
  • Escherichia
  • Why do polychlorinated biphenyls (PCBs) resist electrophilic attack by oxygenases?

    <p>Because of their halogenated structure</p> Signup and view all the answers

    What is a key benefit of reductive attack by anaerobic bacteria on halogenated xenobiotics?

    <p>It leads to mineralization by aerobic bacteria</p> Signup and view all the answers

    What happens to the co-substrate during co-metabolic degradation?

    <p>It serves as a substrate for other organisms</p> Signup and view all the answers

    Which of the following represents a pathway for the degradation of aromatic hydrocarbons?

    <p>Isomerization and oxygenolytic ring cleavage</p> Signup and view all the answers

    What mechanism allows for the introduction of new substituent groups onto the benzene ring?

    <p>Electrophilic attack</p> Signup and view all the answers

    What is the primary condition required for reductive dehalogenation of PCBs?

    <p>Anaerobic conditions</p> Signup and view all the answers

    What type of microorganisms are primarily involved in anaerobic dechlorination?

    <p>Anaerobic bacteria</p> Signup and view all the answers

    What is produced by the incomplete anaerobic dechlorination of PCBs?

    <p>Di- and monochlorinated biphenyls</p> Signup and view all the answers

    Which cellular process begins with glucose degradation in aerobes?

    <p>Cellular respiration process</p> Signup and view all the answers

    Which statement is true regarding aerobic degradation compared to anaerobic processes?

    <p>Aerobic degradation leads to more complete digestion of waste.</p> Signup and view all the answers

    What types of enzymes primarily catalyze the reactions in aerobic biodegradation?

    <p>Oxygenases and peroxidases</p> Signup and view all the answers

    Which class of organisms is primarily responsible for degrading lignin?

    <p>Higher fungi</p> Signup and view all the answers

    Which organic pollutants are rapidly degraded under aerobic conditions?

    <p>Petroleum hydrocarbons and chlorinated aliphatics</p> Signup and view all the answers

    What is the primary role of hydrogen in the acetogenesis phase?

    <p>To thermodynamically enable the conversion of acids</p> Signup and view all the answers

    Which type of bacteria is responsible for the conversion of hydrogen and acetic acid to methane in the methanogenesis phase?

    <p>Methanogenic bacteria</p> Signup and view all the answers

    What happens if the activity of fermentative organisms exceeds that of carboxylic acid degraders and methanogens?

    <p>Acids and hydrogen will accumulate</p> Signup and view all the answers

    In what condition do chlorinated hydrocarbons (CHCs) primarily degrade?

    <p>Under anaerobic conditions</p> Signup and view all the answers

    What is the optimum pH for most methanogens?

    <p>Around 7</p> Signup and view all the answers

    What type of pollutants do volatile organic compounds (VOCs) primarily affect?

    <p>Land and groundwater</p> Signup and view all the answers

    Which of the following products is NOT generated during methanogenesis?

    <p>Hydrogen sulfide</p> Signup and view all the answers

    What are the two classes of VOCs contributing to environmental contamination?

    <p>Petroleum hydrocarbons and chlorinated hydrocarbons</p> Signup and view all the answers

    What is one significant benefit of using biodegradable and eco-friendly plastics?

    <p>They reduce the need for recycling and incineration.</p> Signup and view all the answers

    Which of the following polymers is notable for its ability to be mineralized by microorganisms?

    <p>Polyvinyl alcohol</p> Signup and view all the answers

    Which microorganisms are most commonly involved in the biodegradation of Polyvinyl alcohol?

    <p>Bacillus, Pseudomonas, and Alcaligenes</p> Signup and view all the answers

    What is the primary outcome of Polyhydroxyalkanoates (PHAs) degradation in aerobic conditions?

    <p>Degradation to CO2 and water</p> Signup and view all the answers

    Which characteristic distinguishes Polyvinyl alcohol from other bulk polymers?

    <p>It can achieve thermoplasticity.</p> Signup and view all the answers

    What is a major challenge associated with the disposal of conventional plastics?

    <p>They can accumulate without proper disposal methods.</p> Signup and view all the answers

    Which of the following statements is true regarding the biodegradation mechanism of Polyvinyl alcohol?

    <p>It is accelerated by specific oxidase and dehydrogenase enzymes.</p> Signup and view all the answers

    What affects the recycling efficiency of many conventional plastics?

    <p>The presence of additives during manufacture.</p> Signup and view all the answers

    What is a primary characteristic of contact pesticides?

    <p>They kill pests instantly after being contacted.</p> Signup and view all the answers

    How do systemic pesticides function?

    <p>They are absorbed and then spread throughout the organism.</p> Signup and view all the answers

    Which type of pesticide specifically interferes with insect growth?

    <p>Insect growth regulators</p> Signup and view all the answers

    What role do repellents play in pest control?

    <p>They repel pests rather than killing them.</p> Signup and view all the answers

    Which of the following describes desiccants in relation to pest control?

    <p>They remove water from the bodies of plants or animals.</p> Signup and view all the answers

    What was the original purpose of introducing the cane toad to Australia?

    <p>To eat the beetles attacking sugar cane crops</p> Signup and view all the answers

    What has been the impact of the cane toad becoming an invasive species?

    <p>Decreased populations of native animals</p> Signup and view all the answers

    What is one disadvantage of biological pest control compared to chemical pest control?

    <p>It can take longer to show results</p> Signup and view all the answers

    What are biopesticides primarily produced from?

    <p>Living organisms</p> Signup and view all the answers

    What is Bacillus thuringiensis commonly known as?

    <p>Bt</p> Signup and view all the answers

    How do plant-incorporated protectants (PIPs) function?

    <p>They are produced internally by genetically altered plants</p> Signup and view all the answers

    What happens once a predator population is established through biological pest control?

    <p>It operates autonomously without further human input</p> Signup and view all the answers

    What is the role of the proteins produced by different strains of Bt?

    <p>To kill specific species of insect larvae</p> Signup and view all the answers

    Study Notes

    Kinetic Studies of Biodegradation

    • Thousands of manufactured chemicals and materials are used, their ultimate fate in the environment often unknown
    • These substances are discharged into the environment through air, water, or land
    • Solid waste disposal is a significant environmental problem
    • Many substances degrade slowly, harming plants and animals
    • Biodegradation, aerobic or anaerobic, breaks down large molecules into smaller ones
    • Biodegradation can be used to mitigate pollution and produce biogas
    • Agricultural waste and organic matter are significant renewable energy sources
    • Untreated animal manure is a major pollutant of air and water

    Mechanisms of Biodegradation

    • Cellulose, lignocellulose, and lignin are major plant biomass sources, polymeric substances crucial for the carbon cycle
    • These polymers are degraded by various microorganisms producing enzymes
    • Fungal and bacterial degradation can occur extracellularly
    • Hydrolytic enzymes are responsible for cellulose and hemicellulose degradation
    • Oxidative ligninolytic enzymes depolymerize lignin
    • Many chemicals require different microorganisms and enzymes for degradation
    • Growth-associated degradation uses organic compounds for carbon and energy, leading to complete mineralization. Archaea, prokaryotes, and eukaryotes play a role
    • Co-metabolism involves metabolism of an organic compound alongside a growth substrate. Final products are often CO2 and methane
    • Growth-associated degradation of aliphatic compounds produces CO2, H2O, and cell biomass

    Growth-Associated Degradation of Aliphatic Compounds

    • Cell biomass acts as biocatalysts for degradation
    • Contaminated areas ultimately mineralize cell biomass
    • Aromatic hydrocarbons (benzene, toluene, ethylbenzene, xylenes, naphthalene) are important industrial chemicals and fuels
    • Phenols and chlorophenols are used in various industries

    Growth-Associated Degradation of Aromatic Compounds

    • Many microorganisms utilize catabolic pathways to degrade aromatic compounds
    • Benzene oxidation begins by hydroxylation catalyzed by dioxygenase, leading to a diol converted into catechol by a dehydrogenase
    • Monooxygenase and dioxygenase reactions are important mechanism steps
    • Hydroxylation, oxygenolytic ring cleavage, isomerization and hydrolysis are common reactions for degrading aromatic substrates
    • Bacteria with induced enzyme activity can adapt to diverse substrate mixtures and high degradation rates

    Co-metabolic Degradation of Organopollutants

    • Co-metabolism is an important aspect of microbial transformation
    • Microorganisms that grow on one substrate can also oxidize another
    • Co-substrates are not incorporated; however, their metabolites can serve as a substrate for other organisms in mixed cultures
    • Enzymes and cofactors are involved in co-metabolic transformations
    • Oxygenases and other enzymes are necessary for the conversion of organic compounds to natural intermediates like catechol and protocatechuate

    Aerobic Biodegradation

    • Many microorganisms grow in aerobic conditions using cellular respiration (CSP)
    • Glucose is degraded into smaller molecules in the cytoplasm, which then enter mitochondria for aerobic respiration
    • Oxygen is crucial for breaking down molecules into water and carbon dioxide
    • Aerobic biodegradation is characterized by oxidation reactions, often catalyzed by oxygenases and peroxidases

    Anaerobic Biodegradation

    • Anaerobic biodegradation occurs in the absence of oxygen and various microbes have different roles
    • Electrons originate from the oxidation of organic matter
    • Manganese, iron, sulfur, sulfate, nitrate, and carbon dioxide are important electron acceptors
    • Biogas generation from anaerobic digestion of organic matter is an effective and important renewable energy source
    • Hydrolysis, acidogenesis, acetogenesis, and methanogenesis are the major steps of anaerobic digestion.
    • These stages result in the production of methane and carbon dioxide

    Biodegradation of Industrial Organic Pollutants

    • The fate of industrial chemicals in the environment is important for designing biodegradation systems. Organic pollutants are toxic and accumulate in the environment.
    • Petroleum hydrocarbons (PHCs) and chlorinated hydrocarbons (CHCs) are major pollutants
    • PHCs degrade effectively under aerobic conditions
    • CHCs degrade effectively under anaerobic conditions.

    Biodegradation of Plastics

    • Some plastics are resistant to biodegradation.
    • Polycyclic aromatic hydrocarbons (PAHs) are toxic, mutagenic, and resistant to breakdown.
    • Microbial communities and factors such as environmental conditions, nutrient levels, and temperature influence the rate and extent of plastic biodegradation
    • Polyvinyl alcohol (PVA) is biodegradable, but this does not automatically mean it is bio-based.

    The Pest Problem

    • Pests damage crops, livestock, and property
    • Pesticides are used to control pests, but they can have negative environmental and health consequences
    • Biological pest control utilizes other organisms to manage pests. No chemical contamination, so no harm to the environment
    • "Classical" biological control introduces natural predators, parasites, or pathogens of a target
    • "Conservation" control enhances local natural enemies of the pest
    • "Augmentation" control increases the number of existing pest enemies
    • Biopesticides are produced from living organisms and are safer for humans than chemical pesticides
    • Microbial pesticides, plant-incorporated protectants (PIPs), and biochemical pesticides are some types of biopesticides

    Additional Notes (Categorized by Topic)

    • Classifying pesticides: Pesticides are categorized by the organism they target (e.g., insects, weeds, rodents) or by their chemical structure (e.g., organophosphates, pyrethroids)
    • Possible Health Effects: Pesticides have various effects on humans, with potential long-term or acute symptoms. Serious cases could lead to death.
    • Integrated Pest Management (IPM): IPM uses a combination of techniques (natural methods, biological control, and, if absolutely necessary, pesticides) to minimize pest-related problems and environmental concerns.
    • Disadvantages of biological pest control: Unforeseen population effects, potential new pest introduction from another region
    • Biodegradation of petroleum hydrocarbons: PHCs degrade quickly aerobically. In contrast, Chlorinated hydrocarbons degrade slowly in anaerobic conditions.
    • Biodegradation of aromatic hydrocarbons: Bacteria and fungi can utilize catabolic pathways.
    • Biodegradation of plastics: Bio-based plastics are an alternative to traditional petro-based plastics, but not all bio-plastics are biodegradable.

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    Description

    Explore the complex processes involved in the biodegradation of various substances in the environment. This quiz covers mechanisms such as aerobic and anaerobic breakdown and the role of microorganisms in degrading polymers found in plant biomass. Understand how biodegradation can help mitigate pollution and produce renewable energy.

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