20 Questions
Which DNA repair mechanism could result in mutations due to several bases being deleted or added?
Nonhomologous end joining (NHEJ)
What is the function of zinc finger nucleases (ZFNs)?
To anchor zinc finger DBD to engineered nuclease FokI
What is the purpose of CRISPR-Cas9 bacterial system?
To recognize and destroy viral DNA
What is the role of tracrRNA in the CRISPR-Cas9 system?
To orient the Cas9 protein and RNAse
What is the function of single guide RNA (sgRNA) in the CRISPR-Cas9 system?
To synthesize RNA constructs that target specific genes
What is the advantage of CRISPR-Cas9 over Zinc finger nucleases (ZFNs) and Transcription Activator-like effector nucleases (TALENs)?
Simple customizability
What is the function of gene drives?
To bias inheritance and potentially suppress or replace populations
What is the potential use of gene drive mosquitoes engineered to fight malaria?
To bias inheritance and potentially suppress or replace populations
What is the purpose of homologous recombination in gene editing?
To repair dsDNA damage using a homologous template
What are the variants of CRISPR?
Gene silencing or enhanced transcription
What is the mechanism of genome editing using zinc finger nucleases (ZFNs)?
ZFNs anchor to FokI endonuclease to make a nonspecific double-stranded break
What is the function of TALENs in gene editing?
TALENs recognize a specific nucleotide triplet and cleave the DNA at that site
What is the DNA repair mechanism that can result in mutations due to several bases being deleted or added?
Nonhomologous end joining (NHEJ)
What is the role of homologous recombination in gene editing?
It copies from a homologous template to fix dsDNA damage
Which of the following is a requirement for Cas9 to recognize and cleave the target DNA in the CRISPR-Cas9 system?
Protospacer adjacent motif (PAM)
What is the function of the sgRNA scaffold in the CRISPR-Cas9 system?
It associates with transcribed RNA product to orient the Cas9 protein
What is the function of CRISPR-Cas9 in bacteria?
It acts as an adaptive immune defense against invading viruses
What is the potential use of gene drive mosquitoes engineered to fight malaria?
Population suppression or replacement
What is the advantage of CRISPR-Cas9 over Zinc finger nucleases (ZFNs) and Transcription Activator-like effector nucleases (TALENs)?
Simple customizability
What is the mechanism of gene editing using TALENs?
TALENs recognize a specific nucleotide triplet and cleave the DNA at that site
Test your knowledge on gene knock-in, genome editing, and genetic engineering with this quiz. Learn about genome editing mechanisms like zinc finger nucleases (ZFNs) and TALENS, and understand the differences between in situ alteration of DNA sequence and other genetic engineering techniques.
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