Podcast
Questions and Answers
Which DNA repair mechanism could result in mutations due to several bases being deleted or added?
Which DNA repair mechanism could result in mutations due to several bases being deleted or added?
- Nonhomologous end joining (NHEJ) (correct)
- Zinc finger nucleases (ZFNs)
- Homology-directed repair (HDR)
- Transcription Activator-like effector nucleases (TALENs)
What is the function of zinc finger nucleases (ZFNs)?
What is the function of zinc finger nucleases (ZFNs)?
- To induce gene silencing or enhance transcription
- To fix dsDNA damage by copying from a homologous template
- To recognize a specific nucleotide triplet
- To anchor zinc finger DBD to engineered nuclease FokI (correct)
What is the purpose of CRISPR-Cas9 bacterial system?
What is the purpose of CRISPR-Cas9 bacterial system?
- To synthesize RNA constructs that target specific genes
- To recognize and destroy viral DNA (correct)
- To incorporate viral DNA into bacterial cells
- To repair dsDNA damage using a homologous template
What is the role of tracrRNA in the CRISPR-Cas9 system?
What is the role of tracrRNA in the CRISPR-Cas9 system?
What is the function of single guide RNA (sgRNA) in the CRISPR-Cas9 system?
What is the function of single guide RNA (sgRNA) in the CRISPR-Cas9 system?
What is the advantage of CRISPR-Cas9 over Zinc finger nucleases (ZFNs) and Transcription Activator-like effector nucleases (TALENs)?
What is the advantage of CRISPR-Cas9 over Zinc finger nucleases (ZFNs) and Transcription Activator-like effector nucleases (TALENs)?
What is the function of gene drives?
What is the function of gene drives?
What is the potential use of gene drive mosquitoes engineered to fight malaria?
What is the potential use of gene drive mosquitoes engineered to fight malaria?
What is the purpose of homologous recombination in gene editing?
What is the purpose of homologous recombination in gene editing?
What are the variants of CRISPR?
What are the variants of CRISPR?
What is the mechanism of genome editing using zinc finger nucleases (ZFNs)?
What is the mechanism of genome editing using zinc finger nucleases (ZFNs)?
What is the function of TALENs in gene editing?
What is the function of TALENs in gene editing?
What is the DNA repair mechanism that can result in mutations due to several bases being deleted or added?
What is the DNA repair mechanism that can result in mutations due to several bases being deleted or added?
What is the role of homologous recombination in gene editing?
What is the role of homologous recombination in gene editing?
Which of the following is a requirement for Cas9 to recognize and cleave the target DNA in the CRISPR-Cas9 system?
Which of the following is a requirement for Cas9 to recognize and cleave the target DNA in the CRISPR-Cas9 system?
What is the function of the sgRNA scaffold in the CRISPR-Cas9 system?
What is the function of the sgRNA scaffold in the CRISPR-Cas9 system?
What is the function of CRISPR-Cas9 in bacteria?
What is the function of CRISPR-Cas9 in bacteria?
What is the potential use of gene drive mosquitoes engineered to fight malaria?
What is the potential use of gene drive mosquitoes engineered to fight malaria?
What is the advantage of CRISPR-Cas9 over Zinc finger nucleases (ZFNs) and Transcription Activator-like effector nucleases (TALENs)?
What is the advantage of CRISPR-Cas9 over Zinc finger nucleases (ZFNs) and Transcription Activator-like effector nucleases (TALENs)?
What is the mechanism of gene editing using TALENs?
What is the mechanism of gene editing using TALENs?
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