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Questions and Answers

Which DNA repair mechanism could result in mutations due to several bases being deleted or added?

  • Nonhomologous end joining (NHEJ) (correct)
  • Zinc finger nucleases (ZFNs)
  • Homology-directed repair (HDR)
  • Transcription Activator-like effector nucleases (TALENs)

    • What is the function of zinc finger nucleases (ZFNs)?

  • To induce gene silencing or enhance transcription
  • To fix dsDNA damage by copying from a homologous template
  • To recognize a specific nucleotide triplet
  • To anchor zinc finger DBD to engineered nuclease FokI (correct)

    • What is the purpose of CRISPR-Cas9 bacterial system?

  • To synthesize RNA constructs that target specific genes
  • To recognize and destroy viral DNA (correct)
  • To incorporate viral DNA into bacterial cells
  • To repair dsDNA damage using a homologous template

    • What is the role of tracrRNA in the CRISPR-Cas9 system?

    <p>To orient the Cas9 protein and RNAse</p> Signup and view all the answers

    What is the function of single guide RNA (sgRNA) in the CRISPR-Cas9 system?

    <p>To synthesize RNA constructs that target specific genes</p> Signup and view all the answers

    What is the advantage of CRISPR-Cas9 over Zinc finger nucleases (ZFNs) and Transcription Activator-like effector nucleases (TALENs)?

    <p>Simple customizability</p> Signup and view all the answers

    What is the function of gene drives?

    <p>To bias inheritance and potentially suppress or replace populations</p> Signup and view all the answers

    What is the potential use of gene drive mosquitoes engineered to fight malaria?

    <p>To bias inheritance and potentially suppress or replace populations</p> Signup and view all the answers

    What is the purpose of homologous recombination in gene editing?

    <p>To repair dsDNA damage using a homologous template</p> Signup and view all the answers

    What are the variants of CRISPR?

    <p>Gene silencing or enhanced transcription</p> Signup and view all the answers

    What is the mechanism of genome editing using zinc finger nucleases (ZFNs)?

    <p>ZFNs anchor to FokI endonuclease to make a nonspecific double-stranded break</p> Signup and view all the answers

    What is the function of TALENs in gene editing?

    <p>TALENs recognize a specific nucleotide triplet and cleave the DNA at that site</p> Signup and view all the answers

    What is the DNA repair mechanism that can result in mutations due to several bases being deleted or added?

    <p>Nonhomologous end joining (NHEJ)</p> Signup and view all the answers

    What is the role of homologous recombination in gene editing?

    <p>It copies from a homologous template to fix dsDNA damage</p> Signup and view all the answers

    Which of the following is a requirement for Cas9 to recognize and cleave the target DNA in the CRISPR-Cas9 system?

    <p>Protospacer adjacent motif (PAM)</p> Signup and view all the answers

    What is the function of the sgRNA scaffold in the CRISPR-Cas9 system?

    <p>It associates with transcribed RNA product to orient the Cas9 protein</p> Signup and view all the answers

    What is the function of CRISPR-Cas9 in bacteria?

    <p>It acts as an adaptive immune defense against invading viruses</p> Signup and view all the answers

    What is the potential use of gene drive mosquitoes engineered to fight malaria?

    <p>Population suppression or replacement</p> Signup and view all the answers

    What is the advantage of CRISPR-Cas9 over Zinc finger nucleases (ZFNs) and Transcription Activator-like effector nucleases (TALENs)?

    <p>Simple customizability</p> Signup and view all the answers

    What is the mechanism of gene editing using TALENs?

    <p>TALENs recognize a specific nucleotide triplet and cleave the DNA at that site</p> Signup and view all the answers

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