ISSRs
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Questions and Answers

Which of the following is a main advantage of ISSRs?

  • They are randomly distributed throughout the genome. (correct)
  • They do not require PCR for analytical procedures.
  • They require sequence data for primer construction.
  • They require high quantities of template DNA for PCR.

Which of the following is NOT required for primer construction in ISSRs?

  • Low quantities of template DNA
  • Analytical procedures
  • PCR
  • Sequence data (correct)

Which of the following is true about the quantity of template DNA required for ISSRs?

  • No template DNA is required.
  • High quantities are needed.
  • The quantity required depends on the genome size.
  • Low quantities are needed. (correct)

Study Notes

ISSRs (Inter Simple Sequence Repeats)

  • Main advantage of ISSRs: ISSRs are a simple and cost-effective method for genetic analysis.
  • Not required for primer construction: Prior sequence knowledge is NOT required for primer construction in ISSRs.
  • Template DNA requirement: Only a small quantity of template DNA is required for ISSRs, making it a useful method for analyzing limited DNA samples.

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Test your knowledge on ISSRs, a molecular marker technique that does not require sequence data for primer construction. Learn about the advantages and applications of ISSRs in genetic research.

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