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ISSRs

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Questions and Answers

Which of the following is a main advantage of ISSRs?

They are randomly distributed throughout the genome. (correct)

They do not require PCR for analytical procedures.

They require sequence data for primer construction.

They require high quantities of template DNA for PCR.

Which of the following is NOT required for primer construction in ISSRs?

Low quantities of template DNA

Analytical procedures

PCR

Sequence data (correct)

Which of the following is true about the quantity of template DNA required for ISSRs?

No template DNA is required.

High quantities are needed.

The quantity required depends on the genome size.

Low quantities are needed. (correct)

Study Notes

ISSRs (Inter Simple Sequence Repeats)

Main advantage of ISSRs: ISSRs are a simple and cost-effective method for genetic analysis.
Not required for primer construction: Prior sequence knowledge is NOT required for primer construction in ISSRs.
Template DNA requirement: Only a small quantity of template DNA is required for ISSRs, making it a useful method for analyzing limited DNA samples.

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Description

Test your knowledge on ISSRs, a molecular marker technique that does not require sequence data for primer construction. Learn about the advantages and applications of ISSRs in genetic research.