Podcast
Questions and Answers
What is the primary source material for the Moringa oleifera food supplement used in the experiment?
What is the primary source material for the Moringa oleifera food supplement used in the experiment?
- A mixture of fresh Moringa leaves and fruits
- Hydrated and processed Moringa stems
- Dehydrated pulverized leaves of Moringa oleifera (correct)
- Fresh, unpulverized Moringa roots
In column chromatography, what is the crucial role of the glass column?
In column chromatography, what is the crucial role of the glass column?
- To measure the refractive index of the eluent
- To mix the sample with the mobile phase
- To heat the mobile phase for faster separation
- To hold the solid stationary phase (correct)
During column chromatography, how are the different components of a sample separated?
During column chromatography, how are the different components of a sample separated?
- Based on their boiling points and volatility
- Based on their color and UV absorbance
- Based on their differences in affinity to both the stationary and the mobile phase (correct)
- Based on their molecular weight and size
During the column chromatography procedure, when should the collection beaker be changed?
During the column chromatography procedure, when should the collection beaker be changed?
What is the main purpose of performing Thin Layer Chromatography (TLC) on each fraction collected during column chromatography?
What is the main purpose of performing Thin Layer Chromatography (TLC) on each fraction collected during column chromatography?
In the experiment, what is the stated criteria for stopping fraction collection?
In the experiment, what is the stated criteria for stopping fraction collection?
What specific materials are used as the stationary phase in this experiment?
What specific materials are used as the stationary phase in this experiment?
What is the mobile phase used in this experiment?
What is the mobile phase used in this experiment?
What is used in place of glass wool in this experiment?
What is used in place of glass wool in this experiment?
What is the developing solvent used for TLC analysis of collected fractions?
What is the developing solvent used for TLC analysis of collected fractions?
Based on the information provided, which stationary phase showed better performance in separating the components of the Moringa extract?
Based on the information provided, which stationary phase showed better performance in separating the components of the Moringa extract?
What is mentioned as a limitation of using sodium bicarbonate ($NaHCO_3$) as the stationary phase?
What is mentioned as a limitation of using sodium bicarbonate ($NaHCO_3$) as the stationary phase?
What is the purpose of using a Pasteur pipette in the experiment?
What is the purpose of using a Pasteur pipette in the experiment?
Why is it important to keep adding new solvent during column chromatography?
Why is it important to keep adding new solvent during column chromatography?
What is the relationship between a compound's affinity for the stationary phase and its movement through the column?
What is the relationship between a compound's affinity for the stationary phase and its movement through the column?
What is the main advantage of using column chromatography to isolate components of Moringa capsules?
What is the main advantage of using column chromatography to isolate components of Moringa capsules?
How does the solvent choice impact the separation of compounds in column chromatography?
How does the solvent choice impact the separation of compounds in column chromatography?
What is the significance of Rf values obtained from TLC analysis?
What is the significance of Rf values obtained from TLC analysis?
For TLC, if a compound has a high affinity for the mobile phase, would you expect its Rf value to be closer to 0 or 1?
For TLC, if a compound has a high affinity for the mobile phase, would you expect its Rf value to be closer to 0 or 1?
In the context of conducting this experiment, why is it beneficial to evaluate the effect of changing the stationary phase?
In the context of conducting this experiment, why is it beneficial to evaluate the effect of changing the stationary phase?
Flashcards
Moringana Supplement
Moringana Supplement
A food supplement derived from dehydrated, pulverized leaves of Moringa oleifera (a.k.a. malunggay).
Column Chromatography
Column Chromatography
A separation technique used to isolate a considerable amount of the various components of a food supplement.
Stationary Phase
Stationary Phase
The sample is applied to the top of this and then eluted with the chosen liquid mobile phase.
Mobile Phase
Mobile Phase
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Separation in Column Chromatography
Separation in Column Chromatography
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Objective of Experiment
Objective of Experiment
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Isolate Moringana's components
Isolate Moringana's components
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Effect of Stationary Phase
Effect of Stationary Phase
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Stationary Phase Components
Stationary Phase Components
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Mobile Phase Composition
Mobile Phase Composition
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Type of glass column used
Type of glass column used
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Number of Spots
Number of Spots
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TLC Stationary Phase
TLC Stationary Phase
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Alternative Stationary Phase
Alternative Stationary Phase
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Limitation of sodium bicarbonate
Limitation of sodium bicarbonate
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Study Notes
- Experiment 4 focuses on isolating the components of moringa capsules using column chromatography.
- Moringa capsules contain dehydrated pulverized leaves of Moringa oleifera, also known as malunggay, and are considered a food supplement.
Column Chromatography
- Column chromatography can isolate a considerable amount of various components of a food supplement.
- A glass column holds the solid stationary phase.
- The sample is applied to the top of the stationary phase and then eluted using a chosen liquid mobile phase.
- Components separate based on differences in affinity to both the stationary and mobile phases.
- Components more attracted to the stationary phase are carried away less easily by the mobile phase.
Experiment Objectives
- Setting up and running a column chromatography proficiently.
- Isolating the different components of Moringana food supplement.
- Evaluating the effect of changing the stationary phase on the purification process.
Procedure Reminders
- Stationary phase: silica gel and NaHCO3
- Mobile phase: 4:1 hexane/EtOAc
- Glass column: Pasteur pipette
- Cotton is used instead of glass wool.
- TLC analysis is to be performed for each collected fraction.
- Developing solvent is 4:1 hexane/EtOAc.
- Fraction collection stops after obtaining 4 spots with different Rf values.
Observations
- Silica gel performed better, resulting in 9 spots, while NaHCO3 gave 6 spots.
- NaHCO3 stationary phase cannot be used if the compounds to be separated are acidic.
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