Isoelectric Focusing and Biomolecule Isoelectric Point Quiz

The pH of the surrounding environment can affect the net charge of a molecule by causing it to become more positively or negatively charged due to the gain or loss of protons (H+).

The pI value can affect the solubility of a molecule at a given pH. Molecules have minimum solubility in water or salt solutions at the pH that corresponds to their pI and often precipitate out of solution.

Biological amphoteric molecules such as proteins carry a net positive charge at a pH below their pI, and a net negative charge at a pH above their pI.

<p>Proteins can be separated by net charge using preparative gel electrophoresis, which uses a constant pH, or isoelectric focusing, which uses a pH gradient to separate proteins.</p> Signup and view all the answers

<p>Isoelectric focusing is the process of separating proteins based on their isoelectric point (pI) in a pH gradient. Proteins migrate until they reach a pH equivalent to their pI, where they have no net charge and thus stop migrating. This allows for the separation of proteins based on their pI values and is a crucial step in 2-D gel electrophoresis for analyzing complex protein mixtures.</p> Signup and view all the answers

<p>The specific pI of a protein is used to design the pH gradient in isoelectric focusing, allowing for the separation and purification of the protein from a complex mixture. At its pI, a protein has no net charge and does not bind to any ion exchangers, facilitating its isolation.</p> Signup and view all the answers

<p>Zwitterionic behavior refers to the presence of both positive and negative charges in a molecule, as seen in biological proteins which contain both amine and carboxyl groups. This behavior results in proteins having a variable net charge depending on the pH of the environment, making their separation and purification possible through techniques such as isoelectric focusing and ion exchange chromatography.</p> Signup and view all the answers

<p>The pI of an amino acid can be calculated using the mean of the pKa values of its amine and carboxyl groups. This can be expressed mathematically as: pI = $\frac{1}{2}(pKa_{amine} + pKa_{carboxyl})$.</p> Signup and view all the answers

<p>The pH of the electrophoretic gel determines the net charge of proteins and thus their migration. At a pH lower than the protein's pI, the protein carries a net positive charge and migrates towards the negative electrode. Conversely, at a pH higher than the protein's pI, the protein carries a net negative charge and migrates towards the positive electrode.</p> Signup and view all the answers

<p>Ion exchange chromatography separates proteins based on their net charge at a specific pH. Positively charged proteins bind to negatively charged exchangers at low pH, while negatively charged proteins bind to positively charged exchangers at high pH. This allows for the selective isolation of proteins based on their charge properties.</p> Signup and view all the answers

<p>Algorithms and databases have been developed to estimate the isoelectric points of peptides and proteins based on their amino acid sequences and properties. These computational tools aid in predicting the pI values of biomolecules, facilitating research and purification processes.</p> Signup and view all the answers

<p>Two-dimensional gel electrophoresis separates proteins first by their pI using isoelectric focusing and then by their molecular weight using SDS-PAGE. This technique enables the visualization of proteins as distinct spots on the gel, each representing a different combination of pI and molecular weight.</p> Signup and view all the answers

<p>Metal oxide ceramics with isoelectric points are used in material science for aqueous processing to stabilize suspensions and control the behavior of charged particles. By adjusting the pH of the environment, these ceramics can manipulate the charge properties of particles, influencing their aggregation and dispersion in solutions.</p> Signup and view all the answers

<p>At a pH equal to the protein's pI, the protein carries no net charge and does not bind to any ion exchanger. This allows for the elution of the protein from the chromatography column, as it is not retained by the charged matrix. At pH values different from the protein's pI, the net charge of the protein influences its binding to the ion exchanger, enabling its selective separation.</p> Signup and view all the answers

<p>Buffers with varying pH are used to create the necessary pH gradient for techniques such as isoelectric focusing and ion exchange chromatography. By adjusting the pH of the environment, proteins can be selectively separated and purified based on their charge properties, facilitating the isolation of specific biomolecules from complex mixtures.</p> Signup and view all the answers

<p>At low pH, positively charged proteins bind to negatively charged matrices in cation exchangers, while at high pH, negatively charged proteins bind to positively charged matrices in anion exchangers. However, at a pH equal to the protein's pI, the protein does not bind to any exchanger and can be eluted out, enabling its purification.</p> Signup and view all the answers