Introduction to Tissue Processing
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Questions and Answers

What is one of the main advantages of using supravital or differential dyes in microscopic examination?

  • They provide a permanent fixation of cells.
  • They allow for the observation of physiologic processes. (correct)
  • They eliminate the need for a microscope.
  • They enhance the structural integrity of tissues.

    • Which microscopy technique is NOT suitable for examining unstained tissue samples?

  • Electron microscopy (correct)
  • Phase-Contrast microscopy
  • Brightfield microscopy
  • Atomic force microscopy

    • What is a major limitation of using this method for diagnosis in living samples?

  • It only examines cellular structures.
  • It necessitates complex procedures.
  • It does not require any preparation.
  • It may lead to observations affected by post-mortem changes. (correct)

    • What happens to anatomical relationships during tissue teasing in solutions like NSS or Ringer's solution?

    <p>They are destroyed during the procedure.</p> Signup and view all the answers

    Which of the following statements about 'squash' preparations is correct?

    <p>They involve the dissection of tissue components.</p> Signup and view all the answers

    Which biopsy method is considered the simplest and least invasive for collecting specimens?

    <p>Fine Needle Aspiration</p> Signup and view all the answers

    What is the main purpose of an excisional biopsy?

    <p>To ensure complete removal of the lesion</p> Signup and view all the answers

    Which of the following specimens has the longest preservation time?

    <p>Slides</p> Signup and view all the answers

    What is a punch biopsy used for?

    <p>To obtain a cylindrical core of tissue samples</p> Signup and view all the answers

    Which statement correctly describes the advantages of preserved tissue examination compared to fresh tissue examination?

    <p>Preserved tissues are more reliable for long-term studies.</p> Signup and view all the answers

    What is the main focus of a histopathology laboratory?

    <p>To study diseases at the tissue level</p> Signup and view all the answers

    Which equipment is typically necessary for the preparation and processing of tissue sections?

    <p>Microtome</p> Signup and view all the answers

    What is the primary purpose of fine needle aspiration in a histopathology laboratory?

    <p>To remove cells from an abnormal area for analysis</p> Signup and view all the answers

    Study Notes

    Desired Learning Outcomes

    • Understand various tissue examination methods, choosing appropriate techniques based on specific samples.
    • Differentiate between types of specimens handled in histopathology.
    • Analyze pros and cons of fresh versus preserved tissue examinations.
    • Recognize the significance of initial steps before tissue processing.
    • Identify essential equipment, glassware, and materials in histopathology, including their applications.

    Types of Materials Tested

    • Autopsy Materials: Used to ascertain cause of death.
    • Surgical Materials: Known as surgical or biopsy materials; provide diagnostic information.

    Types of Biopsy Methods

    • Fine Needle Aspiration:
      • Simplest, minimally invasive technique for biopsies.
      • Ideal for fluid-containing tumors.
    • Core Needle Biopsy:
      • Extracts cells with a small surrounding tissue sample.
    • Incisional Biopsy:
      • Removes a portion of tissue with more surrounding area than core biopsy.
    • Excisional Biopsy:
      • Complete removal of the lesion for diagnosis confirmation.
    • Punch Biopsy:
      • Retrieves a cylindrical core of tissue; sizes range from 2mm to 4mm.
      • Center the lesion during sampling.
    • Shave Biopsy:
      • Involves superficial excision of small tissue fragments from surfaces.
    • Curettings:
      • Tissue or growth removal from body cavities.

    Specimen Storage Durations

    • Specimen: 1 month to 1 year.
    • Tissue Blocks: 3 to 10 years.
    • Slides: Indefinite storage.
    • Records: Maintained permanently.

    Examination Considerations

    • Analyze structural and chemical components, sample quantity, and immediate diagnosis necessity.
    • Fresh tissues do not require fixatives.
    • Use Brightfield or Phase-Contrast microscopes for examination.
    • Staining employs supravital or differential dyes for observing physiological processes like:
      • Motion
      • Mitosis
      • Phagocytosis
      • Pinocytosis
    • Technique is straightforward but has limited scope and shows post-mortem changes like putrefaction and autolysis.

    Tissue Separation Techniques

    • Teasing:
      • Dissection of tissue components using NSS or Ringer's solution.
      • Can be observed stained or unstained; however, anatomical relationships are compromised.
    • Squash Preparation:
      • Not specified in detail but typically involves compressing tissue samples for examination.

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    Quiz Team

    Description

    This quiz covers the fundamental principles of tissue processing, including various examination methods and proper specimen handling in the histopathology laboratory. You will learn to distinguish between fresh and preserved tissue examinations and their respective advantages and disadvantages.

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