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Questions and Answers
What does the term 'tissue processing' refer to?
What does the term 'tissue processing' refer to?
- The treatment of tissues to make them sufficiently firm but flexible for desired tissue sections (correct)
- The soaking of tissues in a solid medium to achieve the desired thickness for sectioning
- The production of tissue sections using a cryostat or freezing microtome
- The use of a device to regulate cryostat or freezing microtome for tissue sectioning
What is the purpose of soaking tissues in a solid medium?
What is the purpose of soaking tissues in a solid medium?
- To produce tissue sections with irregular thickness
- To make the tissues more pliable for sectioning
- To achieve the desired thickness for sectioning (correct)
- To freeze the tissues for preservation
What is another technique used for tissue sectioning apart from soaking in a solid medium?
What is another technique used for tissue sectioning apart from soaking in a solid medium?
- Using a device to regulate cryostat or freezing microtome
- Producing tissue sections using a cryostat or freezing microtome (correct)
- Freezing tissues for preservation
- Soaking tissues in a liquid medium
What is the distinctive feature of fixed soaked tissues?
What is the distinctive feature of fixed soaked tissues?
What does it mean for tissues to be 'sufficiently firm but flexible'?
What does it mean for tissues to be 'sufficiently firm but flexible'?
Flashcards
Tissue Processing
Tissue Processing
Treatment of tissues to make them firm yet flexible enough for sectioning.
Soaking Tissues in Solid Medium
Soaking Tissues in Solid Medium
To provide support so tissues can be cut into thin sections of desired thickness.
Cryostat/Freezing Microtome
Cryostat/Freezing Microtome
Producing tissue sections using a cryostat or freezing microtome
Sufficiently Firm but Flexible
Sufficiently Firm but Flexible
Tissue can be appropriately cut into desired sections
Signup and view all the flashcardsStudy Notes
Tissue Processing
- Tissue processing involves treating tissue to impregnate it into a solid medium, making it firm yet elastic for cutting into desirable thickness sections on a microtome.
- Fixed and impregnated tissues have an advantage in being easily stored and allowing for reproducibility of sections at a later date.
- Alternative techniques for producing tissue sections include cryostat or freezing microtome on frozen tissues.
Importance of Labeling
- Proper labeling of tissue is crucial to avoid confusion, duplication of same names, or incorrect diagnosis for patients.
- A full-proof labeling system is necessary, with the label remaining throughout the entire processing and as a permanent record.
- Most laboratories use a numbering system for each specimen, recording a specific individual number, patient's name, doctor's name, and nature of tissue in the register.
Labeling Precautions
- Ordinary ink should not be used for labeling, as it gets dissolved in the reagent used during processing.
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