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Questions and Answers
What does the term 'tissue processing' refer to?
What is the purpose of soaking tissues in a solid medium?
What is another technique used for tissue sectioning apart from soaking in a solid medium?
What is the distinctive feature of fixed soaked tissues?
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What does it mean for tissues to be 'sufficiently firm but flexible'?
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Study Notes
Tissue Processing
- Tissue processing involves treating tissue to impregnate it into a solid medium, making it firm yet elastic for cutting into desirable thickness sections on a microtome.
- Fixed and impregnated tissues have an advantage in being easily stored and allowing for reproducibility of sections at a later date.
- Alternative techniques for producing tissue sections include cryostat or freezing microtome on frozen tissues.
Importance of Labeling
- Proper labeling of tissue is crucial to avoid confusion, duplication of same names, or incorrect diagnosis for patients.
- A full-proof labeling system is necessary, with the label remaining throughout the entire processing and as a permanent record.
- Most laboratories use a numbering system for each specimen, recording a specific individual number, patient's name, doctor's name, and nature of tissue in the register.
Labeling Precautions
- Ordinary ink should not be used for labeling, as it gets dissolved in the reagent used during processing.
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Description
Test your knowledge of tissue processing in histopathology with this quiz. Explore the techniques used to render tissue firm yet elastic for sectioning and examine the impregnation process into a solid medium
