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Histopathology: Tissue Processing Quiz

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Questions and Answers

What does the term 'tissue processing' refer to?

The treatment of tissues to make them sufficiently firm but flexible for desired tissue sections (correct)

The soaking of tissues in a solid medium to achieve the desired thickness for sectioning

The production of tissue sections using a cryostat or freezing microtome

The use of a device to regulate cryostat or freezing microtome for tissue sectioning

What is the purpose of soaking tissues in a solid medium?

To produce tissue sections with irregular thickness

To make the tissues more pliable for sectioning

To achieve the desired thickness for sectioning (correct)

To freeze the tissues for preservation

What is another technique used for tissue sectioning apart from soaking in a solid medium?

Using a device to regulate cryostat or freezing microtome

Producing tissue sections using a cryostat or freezing microtome (correct)

Freezing tissues for preservation

Soaking tissues in a liquid medium

What is the distinctive feature of fixed soaked tissues?

<p>They are firmer and more flexible for sectioning</p> Signup and view all the answers

What does it mean for tissues to be 'sufficiently firm but flexible'?

<p>The tissues can be appropriately cut into desired sections</p> Signup and view all the answers

Study Notes

Tissue Processing

Tissue processing involves treating tissue to impregnate it into a solid medium, making it firm yet elastic for cutting into desirable thickness sections on a microtome.
Fixed and impregnated tissues have an advantage in being easily stored and allowing for reproducibility of sections at a later date.
Alternative techniques for producing tissue sections include cryostat or freezing microtome on frozen tissues.

Importance of Labeling

Proper labeling of tissue is crucial to avoid confusion, duplication of same names, or incorrect diagnosis for patients.
A full-proof labeling system is necessary, with the label remaining throughout the entire processing and as a permanent record.
Most laboratories use a numbering system for each specimen, recording a specific individual number, patient's name, doctor's name, and nature of tissue in the register.

Labeling Precautions

Ordinary ink should not be used for labeling, as it gets dissolved in the reagent used during processing.

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Description

Test your knowledge of tissue processing in histopathology with this quiz. Explore the techniques used to render tissue firm yet elastic for sectioning and examine the impregnation process into a solid medium