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Questions and Answers
What is the main purpose of a microtome in tissue preparation?
What is the main purpose of a microtome in tissue preparation?
- To remove paraffin wax from tissue
- To stain the tissue sections
- To store the paraffin blocks
- To cut tissue into thin sections (correct)
What is the thickness range of sections produced by a microtome?
What is the thickness range of sections produced by a microtome?
- 1-2 µm
- 10-15 µm
- 3-10 µm (correct)
- 20-25 µm
What process is used to eliminate wrinkles from the tissue ribbon?
What process is used to eliminate wrinkles from the tissue ribbon?
- Covering with a glass slide
- Lowering onto a water bath (correct)
- Rehydration with alcohol
- Staining with Hematoxylin
Which of the following is NOT part of the staining process for tissue sections?
Which of the following is NOT part of the staining process for tissue sections?
What is the function of xylene in the tissue preparation process?
What is the function of xylene in the tissue preparation process?
What color does Hematoxylin stain nuclei in tissue sections?
What color does Hematoxylin stain nuclei in tissue sections?
Which alcohol percentage is directly used before rehydration with water?
Which alcohol percentage is directly used before rehydration with water?
In the preparation of tissue sections, what is the final step after staining?
In the preparation of tissue sections, what is the final step after staining?
What is the primary purpose of microtechniques in histology?
What is the primary purpose of microtechniques in histology?
Which of the following techniques is described as the fastest?
Which of the following techniques is described as the fastest?
What is the first step in the paraffin technique?
What is the first step in the paraffin technique?
Why is fixation important in tissue processing?
Why is fixation important in tissue processing?
What thickness are tissue sections typically cut to during the paraffin technique?
What thickness are tissue sections typically cut to during the paraffin technique?
Which characteristic makes the paraffin technique the most common?
Which characteristic makes the paraffin technique the most common?
What type of tools are recommended for tissue dissection to minimize damage?
What type of tools are recommended for tissue dissection to minimize damage?
In which step of the paraffin technique are tissue samples mounted on glass slides?
In which step of the paraffin technique are tissue samples mounted on glass slides?
What is the primary purpose of a fixative in tissue preparation?
What is the primary purpose of a fixative in tissue preparation?
During which step is bound water removed from the tissue?
During which step is bound water removed from the tissue?
What is the typical concentration progression of alcohol used in the dehydration step?
What is the typical concentration progression of alcohol used in the dehydration step?
What is the role of clearing agents in tissue processing?
What is the role of clearing agents in tissue processing?
At what temperature is melted soft paraffin typically heated for infiltration?
At what temperature is melted soft paraffin typically heated for infiltration?
What is the primary function of embedding in the tissue preparation process?
What is the primary function of embedding in the tissue preparation process?
Why is it important to keep the tissue sample size small during fixation?
Why is it important to keep the tissue sample size small during fixation?
What is the melting point range for hard paraffin used in embedding?
What is the melting point range for hard paraffin used in embedding?
Study Notes
Introduction to Microtechniques
- Microtechniques involve preparation, processing, and examination of histological tissue samples at a microscopic level.
- Essential for preserving tissue structures and highlighting specific cellular components.
Types of Microtechniques
- Paraffin Technique: Most widely used method.
- Celloidine Technique: Known for its perfection.
- Freezing Technique: Fastest method for tissue preparation.
Paraffin Technique Steps
- Tissue Collection: Fresh tissue obtained using sterile blunt-cutting tools to minimize damage, followed by preservation in fixatives.
- Fixation: Chemical preservation to prevent tissue autolysis, enhance dye attachment, and improve optical differentiation. Common fixative is 10% buffered formalin.
- Dehydration: Gradual removal of water using progressively higher concentrations of ethanol.
- Clearing: Replacement of alcohol with a clearing agent like xylene.
- Infiltration/Impregnation: Removal of clearing agent and permeation of tissue with melted paraffin wax heated slightly above melting point.
- Embedding: Placement of infiltrated tissue in a mold filled with hard paraffin to create solid wax blocks for easier sectioning.
Tissue Sectioning and Staining
- Microtomy: Technique for cutting tissue into extremely thin sections (3-10 µm) using a microtome. Sections laid on a water bath to eliminate wrinkles.
- Staining: Reverses the paraffin removal process for dye penetration:
- Use clearing agent followed by rehydration through descending alcohol grades.
- Common stains: Hematoxylin (stains nuclei blue) and Eosin (stains cytoplasm pink).
Preparation Notes
- Emphasize the importance of sample size and fixative volume for optimal preservation.
- Record keeping: Embed tissue with clear labeling on cassette including tissue name, fixative type, and date.
- Ensure proper drying time for slides before staining to maintain integrity of tissue sections.
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Description
This quiz focuses on the practical aspects of the Introduction to Human Biology (IHB102) course at Dubai Medical College for Girls. It covers essential microtechniques used in biological preparation and examination. Prepare to test your understanding and application of these concepts in human biology.
