Introduction to Chromatography
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Questions and Answers

What is the main factor that determines the separation of solutes in chromatography?

  • Width of the solute peaks
  • Type of stationary phase used
  • Volume of the mobile phase
  • Difference in retention of solutes (correct)

    • What type of chromatography involves the use of a support containing fixed charges?

  • Ion-exchange chromatography (correct)
  • Affinity chromatography
  • Size exclusion chromatography
  • Partition chromatography

    • What is the term for the equilibrium constant for the distribution of a solute between the mobile and stationary phases?

  • Volumestationary phase
  • k'
  • KD (correct)
  • Volumemobile phase

    • What is the purpose of having a narrow width of the solute peaks in chromatography?

    <p>To improve the resolution of the separation</p> Signup and view all the answers

    What type of chromatography involves the use of a stationary phase with immobilized ligands?

    <p>Affinity chromatography</p> Signup and view all the answers

    What is the term for the ratio of the concentration of a solute in the stationary phase to its concentration in the mobile phase?

    <p>k'</p> Signup and view all the answers

    What type of chromatography involves a solid, underivatized support as the stationary phase?

    <p>Gas-solid chromatography</p> Signup and view all the answers

    What is the optimal range of k' for separation in chromatography?

    <p>k' = 2-10</p> Signup and view all the answers

    What is the primary basis for dividing chromatographic techniques?

    <p>Type of mobile phase used</p> Signup and view all the answers

    What is the relationship between the strength of analyte interactions with the stationary phase and the time it takes to pass through the system?

    <p>Stronger interactions result in slower passage through the system</p> Signup and view all the answers

    What is the relationship between the capacity factor k’ and the strength of interaction between a solute and the stationary and mobile phases?

    <p>k’ is directly related to the strength of interaction</p> Signup and view all the answers

    What is Wh in the context of chromatography?

    <p>Half-height width of the peak in time units</p> Signup and view all the answers

    What does a chromatogram typically represent?

    <p>Concentration versus elution time</p> Signup and view all the answers

    At what point is equilibrium achieved or approached in a chromatographic peak?

    <p>At the center of the peak</p> Signup and view all the answers

    What is the purpose of a stationary phase in chromatography?

    <p>To interact with the analytes and separate them</p> Signup and view all the answers

    What is the formula for the capacity factor k’?

    <p>k’ = Moles A_stationary phase / Moles A_mobile phase</p> Signup and view all the answers

    What is the main difference between gas chromatography and liquid chromatography?

    <p>Type of mobile phase used</p> Signup and view all the answers

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