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Questions and Answers
What is the main factor that determines the separation of solutes in chromatography?
What is the main factor that determines the separation of solutes in chromatography?
- Width of the solute peaks
- Type of stationary phase used
- Volume of the mobile phase
- Difference in retention of solutes (correct)
What type of chromatography involves the use of a support containing fixed charges?
What type of chromatography involves the use of a support containing fixed charges?
- Ion-exchange chromatography (correct)
- Affinity chromatography
- Size exclusion chromatography
- Partition chromatography
What is the term for the equilibrium constant for the distribution of a solute between the mobile and stationary phases?
What is the term for the equilibrium constant for the distribution of a solute between the mobile and stationary phases?
- Volumestationary phase
- k'
- KD (correct)
- Volumemobile phase
What is the purpose of having a narrow width of the solute peaks in chromatography?
What is the purpose of having a narrow width of the solute peaks in chromatography?
What type of chromatography involves the use of a stationary phase with immobilized ligands?
What type of chromatography involves the use of a stationary phase with immobilized ligands?
What is the term for the ratio of the concentration of a solute in the stationary phase to its concentration in the mobile phase?
What is the term for the ratio of the concentration of a solute in the stationary phase to its concentration in the mobile phase?
What type of chromatography involves a solid, underivatized support as the stationary phase?
What type of chromatography involves a solid, underivatized support as the stationary phase?
What is the optimal range of k' for separation in chromatography?
What is the optimal range of k' for separation in chromatography?
What is the primary basis for dividing chromatographic techniques?
What is the primary basis for dividing chromatographic techniques?
What is the relationship between the strength of analyte interactions with the stationary phase and the time it takes to pass through the system?
What is the relationship between the strength of analyte interactions with the stationary phase and the time it takes to pass through the system?
What is the relationship between the capacity factor k’ and the strength of interaction between a solute and the stationary and mobile phases?
What is the relationship between the capacity factor k’ and the strength of interaction between a solute and the stationary and mobile phases?
What is Wh in the context of chromatography?
What is Wh in the context of chromatography?
What does a chromatogram typically represent?
What does a chromatogram typically represent?
At what point is equilibrium achieved or approached in a chromatographic peak?
At what point is equilibrium achieved or approached in a chromatographic peak?
What is the purpose of a stationary phase in chromatography?
What is the purpose of a stationary phase in chromatography?
What is the formula for the capacity factor k’?
What is the formula for the capacity factor k’?
What is the main difference between gas chromatography and liquid chromatography?
What is the main difference between gas chromatography and liquid chromatography?
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