17 Questions
What is the main factor that determines the separation of solutes in chromatography?
Difference in retention of solutes
What type of chromatography involves the use of a support containing fixed charges?
Ion-exchange chromatography
What is the term for the equilibrium constant for the distribution of a solute between the mobile and stationary phases?
KD
What is the purpose of having a narrow width of the solute peaks in chromatography?
To improve the resolution of the separation
What type of chromatography involves the use of a stationary phase with immobilized ligands?
Affinity chromatography
What is the term for the ratio of the concentration of a solute in the stationary phase to its concentration in the mobile phase?
k'
What type of chromatography involves a solid, underivatized support as the stationary phase?
Gas-solid chromatography
What is the optimal range of k' for separation in chromatography?
k' = 2-10
What is the primary basis for dividing chromatographic techniques?
Type of mobile phase used
What is the relationship between the strength of analyte interactions with the stationary phase and the time it takes to pass through the system?
Stronger interactions result in slower passage through the system
What is the relationship between the capacity factor k’ and the strength of interaction between a solute and the stationary and mobile phases?
k’ is directly related to the strength of interaction
What is Wh in the context of chromatography?
Half-height width of the peak in time units
What does a chromatogram typically represent?
Concentration versus elution time
At what point is equilibrium achieved or approached in a chromatographic peak?
At the center of the peak
What is the purpose of a stationary phase in chromatography?
To interact with the analytes and separate them
What is the formula for the capacity factor k’?
k’ = Moles A_stationary phase / Moles A_mobile phase
What is the main difference between gas chromatography and liquid chromatography?
Type of mobile phase used
This quiz covers the basics of chromatography, including the capacity factor, types of chromatography, and chromatographic techniques. Learn about the fundamental definition of k' and the primary division of chromatographic techniques.
Make Your Own Quizzes and Flashcards
Convert your notes into interactive study material.
Get started for free
