Introduction to Biotechnology

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Questions and Answers

What is the primary focus of biotechnology?

  • The study of human biology only
  • Developing new computer technologies
  • Combining living entities with techniques for economic purposes (correct)
  • The classification of various organisms

What does the term 'Bio' in biotechnology originate from?

  • An English term for biology
  • A Latin term meaning innovation
  • A Greek term meaning life (correct)
  • A French term for crafting

Who is credited with the earliest use of the term 'biotechnology'?

  • Gregor Mendel
  • Károly Ereky (correct)
  • Robert Bud
  • Louis Pasteur

What challenge exists in defining biotechnology?

<p>The field encompasses diverse scientific and production activities (A)</p> Signup and view all the answers

According to the United Nations, what does biotechnology apply to?

<p>Biological systems, living organisms, or their derivatives (D)</p> Signup and view all the answers

What year was the United Nations Convention on Biological Diversity established?

<p>1992 (D)</p> Signup and view all the answers

What aspect is highlighted in the Cartagena Protocol regarding biotechnology?

<p>Distinct aspects of biotechnology (D)</p> Signup and view all the answers

Which of the following is NOT a characteristic of biotechnology?

<p>Completely unrelated to technology (C)</p> Signup and view all the answers

What is the primary advantage of producing therapeutic molecules from genetically modified plants?

<p>They minimize the risk of contamination by pathogens. (D)</p> Signup and view all the answers

Which plant is being researched for the production of a vaccine to prevent gastroenteritis due to E.coli?

<p>Banana (A)</p> Signup and view all the answers

What is one of the applications of enzymatic processes in industrial domains?

<p>Food industry additives (A)</p> Signup and view all the answers

Which of the following is NOT a benefit of using transgenic plants to produce human proteins?

<p>Increased risk of viral contamination (B)</p> Signup and view all the answers

What is one intended result of developing biodegradable lubricants and plastics?

<p>Less environmental pollution (D)</p> Signup and view all the answers

What is a possible use of recombinant human hemoglobin produced from transgenic tobacco plants?

<p>Trauma treatment (D)</p> Signup and view all the answers

Which products can be synthesized from genetically modified plants besides proteins?

<p>Therapeutic molecules and vaccines (A)</p> Signup and view all the answers

Which benefit is commonly associated with using enzymatic processes in industries?

<p>Cleaner and more sustainable processes (A)</p> Signup and view all the answers

Which types of materials are commonly utilized in blue biotechnology?

<p>Hydrocolloids and gelling agents (C)</p> Signup and view all the answers

What is a primary benefit of using transgenic plants in agriculture?

<p>They provide resistance to herbicides and specific insects. (D)</p> Signup and view all the answers

Which bacterium is a significant source of insect resistance genes?

<p>Bacillus thuringiensis (D)</p> Signup and view all the answers

What is a common use of marker molecules derived from marine organisms?

<p>Diagnostics and research (B)</p> Signup and view all the answers

How do transgenic plants achieve resistance to viral infections?

<p>Through the production of proteins that inhibit viral multiplication (D)</p> Signup and view all the answers

Which transgenic plant is mentioned as being resistant to cucumber mosaic virus?

<p>Melons (C)</p> Signup and view all the answers

What is the mode of action of the Cry a gene in corn?

<p>It produces a protein that becomes a toxin in the insect digestive tract. (C)</p> Signup and view all the answers

Which major sectors are exploring the potential of blue biotechnology?

<p>Agriculture and cosmetics (B)</p> Signup and view all the answers

What is the focus of biotechnology as defined by the United Nations Food and Agriculture Organization?

<p>Manipulation of living organisms at the genetic level (A)</p> Signup and view all the answers

During fermentation, what products are typically produced through the degradation of organic matter?

<p>Acids, alcohol, or gas (C)</p> Signup and view all the answers

Which of the following domains has been significantly influenced by the evolution of genetics?

<p>Molecular biology (B)</p> Signup and view all the answers

What technique is used to cultivate cells outside their natural living environment?

<p>Cell culture (A)</p> Signup and view all the answers

According to biotechnological principles, which of the following is NOT a benefit of using fermentation products?

<p>Waste disposal (D)</p> Signup and view all the answers

What role do nucleic acids play in biotechnology?

<p>They can be directly injected into cells (D)</p> Signup and view all the answers

Which process allows for the fusion of cells from different taxonomic families?

<p>Cell fusion techniques (A)</p> Signup and view all the answers

What scientific achievement by Watson and Crick in 1953 was significant for biotechnology?

<p>Elucidation of DNA's chemical nature (C)</p> Signup and view all the answers

What is one of the uses of molecular biology and genetic engineering mentioned?

<p>Synthesis of organic products (A)</p> Signup and view all the answers

Which process involves the metabolization of pollutants by microorganisms?

<p>Bioremediation (A)</p> Signup and view all the answers

What alternative fuel production method is mentioned?

<p>Hydrogen photolysis (B)</p> Signup and view all the answers

Which of the following is a use for enzymes and biocatalysts?

<p>Food processes and medical diagnostics (D)</p> Signup and view all the answers

What is the role of a transgene in creating a transgenic organism?

<p>It introduces a foreign DNA sequence into a cell. (C)</p> Signup and view all the answers

What is one benefit of biological nitrogen fixation?

<p>Reduction of nitrogen fertilizer usage (C)</p> Signup and view all the answers

Why is enzyme immobilization important in biotechnological applications?

<p>It stabilizes enzymes for industrial use. (B)</p> Signup and view all the answers

Which of the following is a technique associated with bioremediation?

<p>Digestion of plant waste via bacteria. (C)</p> Signup and view all the answers

What type of substances can be produced from plant cell cultures?

<p>Biomass and chemical products (D)</p> Signup and view all the answers

Which technology is associated with the development of therapeutics?

<p>Recombinant DNA technology (B)</p> Signup and view all the answers

What characteristic of enzymes typically poses a challenge for their use in industrial biotechnology?

<p>Their instability and loss of catalytic activity. (B)</p> Signup and view all the answers

What ancient biotechnology development occurred around 9000 BC?

<p>Discovery of fermented foods. (B)</p> Signup and view all the answers

What process is involved in wastewater recycling?

<p>Collection and pre-treatment (A)</p> Signup and view all the answers

Which of the following is NOT an outcome of genetic manipulation in bioremediation?

<p>Contamination of soil with heavy metals. (D)</p> Signup and view all the answers

What type of biotechnology involved the production of baker's yeast in 1915?

<p>Classical biotechnology. (B)</p> Signup and view all the answers

What is one function of biosensors in industrial applications?

<p>Detecting pollutants more efficiently. (C)</p> Signup and view all the answers

Flashcards

What is Biotechnology?

A multidisciplinary field combining living organisms with techniques for economic purposes. It's rapidly evolving thanks to advancements in molecular biology.

Origin of 'Biotechnology'

The word 'biotechnology' comes from two Greek words: 'Bios' meaning life and 'Technologia' meaning study of techniques, tools, or machines.

Who coined the term 'Biotechnology' and when?

Hungarian scientist Károly Ereky coined the term 'biotechnology' in 1919, describing the conversion of organic raw materials into useful products.

Challenges in defining Biotechnology

Biotechnology covers a wide range of biological and technological concepts, making a universal definition challenging.

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UN definition of Biotechnology

The United Nations Convention on Biological Diversity defines biotechnology as any technology using biological systems for creating or modifying products. The definition emphasizes using living organisms or their derivatives for specific applications.

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Biotechnology (FAO definition)

The use of living organisms or biological processes to create products or services beneficial to humans.

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Fermentation

A natural process where microorganisms break down organic matter without oxygen, producing valuable substances like alcohol or acids.

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Genetics

The study of genes, heredity and genetic variation in organisms.

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Cell culture

The process of growing cells outside their natural environment, in a controlled laboratory setting.

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Recombinant DNA technology

A type of biotechnology that involves combining genetic material from different organisms.

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Direct injection of nucleic acids

The direct introduction of genetic material into cells or organelles.

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Cell fusion technology

The fusion of cells from different taxonomic families to achieve breeding goals.

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Genetic manipulation

A technique in biotechnology that alters the genetic makeup of organisms to create new products.

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Transgenic Organisms

The process of introducing foreign DNA (transgene) into a cell, enabling the organism to produce new proteins.

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Enzymes

Catalysts in living organisms that speed up specific metabolic reactions, known for their specificity and regulation.

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Enzyme Immobilization

The process of making enzymes stable and usable outside of living organisms, often for industrial purposes.

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Bioremediation

A technique using living organisms to clean up polluted environments by degrading or neutralizing contaminants.

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Ancient Biotechnology

The practice of using living organisms for various purposes, with a long history dating back to fermented foods.

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Classical Biotechnology

A more modern and advanced form of biotechnology, utilizing techniques like genetic engineering, fermentation, and cell culture.

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Transgenic Microorganisms

Microorganisms genetically modified to produce substances like proteins or enzymes with specific applications.

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Enzymatic Engineering

The use of enzymes for various industrial processes, including the production of biofuels and pharmaceuticals.

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Bacillus thuringiensis (Bt)

A bacterium commonly used to create insect-resistant crops. It contains genes expressing insecticidal proteins that target specific insect pests.

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Cry Protein

A protein produced by Bt, toxic to specific insects, but harmless to humans and most other animals. It's often used in genetically modified crops.

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Cry a Gene (Bt Gene)

A gene from Bt, responsible for producing the Cry protein, which provides insect resistance in plants.

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Bt Corn

A genetically modified crop expressing the Cry a gene, making it resistant to the European corn borer.

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Virus-Resistant Plants

Genetically modified plants expressing specific proteins that interfere with virus replication, preventing their spread.

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Disease-Resistant Plants (Fungal/Bacterial)

Genetically modified plants that produce toxins or other compounds that make them resistant to fungal or bacterial infections.

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Transgenesis

The process of introducing new genes into an organism's DNA, often to create a specific trait like disease resistance.

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Biotechnology for Crop Protection

A method of using genes from specific organisms, like bacteria, to create pest-resistant crops through genetic modification.

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Traditional Fermentation in Biotechnology

Traditional fermentation processes, such as alcoholic fermentation, production of organic acids (citric acid, acetic acid, etc.), and production of antibiotics, focus on using microorganisms to create products.

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Enzymes and Biocatalysts in Biotechnology

Enzymes and biocatalysts are naturally occurring proteins that accelerate specific reactions, finding applications in various fields such as food processing (cheesemaking, brewing), chemical synthesis, and medical diagnostics.

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Biotechnology for Sustainable Fuels and Products

Biotechnology seeks alternative sources of fuel and organic products to replace petroleum-based ones. This involves harnessing processes like hydrogen photolysis, biomass digesters for methane production, and alcohol production from plant sugars.

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Interferon and Monoclonal Antibodies in Biotechnology

Interferon and monoclonal antibody technologies have revolutionized medicine, enabling the development of targeted therapies for diseases. They also contribute to the creation of advanced diagnostic equipment.

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Plant Cell Cultures and Single-Cell Proteins

Plant cell cultures offer an alternative for producing biomass and various chemical products, including steroids and alkaloids. Using single-cell protein sources, like microalgae, provides an efficient pathway for biomass production.

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Bioremediation for Waste Treatment

Bioremediation employs microorganisms to clean up pollutants from wastewaters and soils. This environmentally friendly approach helps in pollution control and waste treatment, especially in the agri-food industry.

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Biological Nitrogen Fixation in Agriculture

Biological nitrogen fixation is the process where nitrogen gas from the atmosphere is converted into usable compounds. This process offers an alternative solution for reducing dependency on synthetic nitrogen fertilizers in agriculture and ammonia production.

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What is the purpose of genetically engineered blood products?

A process that uses genetically modified plants, like tobacco or potatoes, to produce proteins that are normally found in human blood, such as albumin or hemoglobin. These proteins are then used in medical treatments.

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What specific blood protein can be produced from transgenic tobacco?

Transgenic tobacco plants can be used to produce human albumin, a protein important in trauma treatment.

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Besides tobacco plants, how can biotechnology be used to produce albumin?

Biotechnology offers a new way to produce human albumin. This approach avoids relying solely on blood plasma donations, which can be problematic due to supply and potential risks.

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What is the idea behind a 'vaccine banana'?

Genetically modified plants like bananas can be used to deliver vaccines, offering a convenient and potentially affordable method for vaccinating populations in developing countries.

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What specific disease is the 'vaccine banana' aimed at preventing?

Scientists are working on developing a vaccine banana to protect people from E.coli bacterial gastroenteritis.

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What is the main advantage of using genetically modified plants to produce vaccines?

Producing therapeutic molecules and vaccines from plants like tobacco, corn, or potatoes can be a safer alternative to traditional production methods, as it eliminates the risk of contamination by human-specific viruses.

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What are enzymatic processes in the context of biotechnology?

Enzymatic processes are environmentally friendly and are used across different industries for various applications involving the breaking down or changing of substances.

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Give examples of different industries utilizing enzymatic processes.

Enzymatic processes are used in starch processing for producing alcohol and sugars, food industry additives for quality improvement, animal feed production, cosmetics, paper manufacturing, tanning, and various chemical applications.

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Study Notes

Introduction to Biotechnology

  • Biotechnology is a multidisciplinary science combining living organisms or parts with techniques for economic gain.
  • The term "Biotechnology" is derived from the Greek words "bios" (life) and "technologia" (study of techniques).
  • The concept of biotechnology evolved from early techniques, such as fermentation, to modern approaches like genetic engineering.
  • Biotechnology encompasses both "old" and "new" technologies.

Definitions of Biotechnology

  • Biotechnology is difficult to define due to diverse scientific and production activities.
  • No single global definition exists.
  • The United Nations Convention on Biological Diversity defines biotechnology as "any technological application that uses biological systems".
  • The FAO (United Nations Food and Agriculture Organization) defines it as using biological processes or organisms to create materials and services, and manipulating living organisms, scientifically, to produce new products, like hormones and vaccines.

Origins of Biotechnologies

  • Fermentation: A natural process of organic matter breakdown by microorganisms, producing acids, alcohols, or gases as byproducts.
  • Genetics: DNA research and spatial organization studies, improving the understanding of inheritance and regulation.
  • Cell Culture: Growing cells outside their natural environment, enabling researchers to study cell function and produce large quantities of desired substances.
  • Recombinant DNA: Inserting DNA fragments of interest into another organism's DNA, with the potential to express those characteristics.
  • Transgenesis/Genetic Engineering: The artificial transfer of genetic material from one organism to another, directly manipulating the DNA of organisms.

Bioremediation

  • A waste management technique using organisms to remove pollutants or neutralize contaminants.
  • Some examples are degrading hydrocarbons and obtaining methane from waste.

Evolution of Biotechnologies

  • Ancient biotechnology, practiced before 1800 CE, involved accidental discovery of fermented foods.
  • Modern biotechnology has techniques for modifying flavors and textures, and producing different products.
  • Classical biotechnology includes the production of different beverages (beer, wine, vinegar).
  • Modern biotechnology makes use of several techniques based on the understanding of genetic material (DNA), including cell culture, recombinant DNA, and modifying cells and plants.

Definitions of Different Types of Biotechnology

  • Red biotechnology: Focuses on medicine and healthcare applications, including vaccines, antibiotics, and gene therapies.
  • White biotechnology: Centers on industrial processes and producing chemicals, fuels, and materials.
  • Green biotechnology: Applies to agriculture, using microorganisms to improve crop yields, and making biofuels.
  • Grey biotechnology: Focuses on environmental applications, like bioremediation and maintaining biodiversity.
  • Blue biotechnology: Focuses on water and marine resources, with applications in medicine and industry.

Typical Biotechnology Products

  • Agrarian Sector: Creating pest-resistant, disease-resistant, herbicide-tolerant, or higher-yielding crops.
  • Industry: Using biological systems to create new materials and optimize industrial processes.
  • Health: Creating vaccines, gene therapies, and producing valuable biochemical compounds.

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