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Questions and Answers
What is the diagnostic test system that evaluates the number of stain zones or visually evaluates the number of interferon gamma-producing T-cells?
System ELISPOT (T-SPOT.TB)
What is the purpose of the IGRA test system?
To detect interferon gamma release by activated memory T-cells in response to stimulation with recombinant proteins ESAT6 and CFPT10.
What is the disadvantage of IGRA test systems that prevents their use in population screening tests for tuberculosis?
High cost, necessary equipped laboratory and specialists, special strict requirements of biological material preparation.
What is the type of analysis used in the QuantiFERON test system?
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What is required for the preparation of biological material for IGRA test systems?
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What is the source of blood required for IGRA test system analysis?
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What are the two proteins used to stimulate interferon gamma release in IGRA test systems?
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What is the role of T-cells in the IGRA test system?
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Why do IGRA test systems require equipped laboratories and specialists?
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What is the limitation of IGRA test systems in terms of their use in children?
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Study Notes
Interferon-Gamma Release Assays (IGRA)
- International studies on the mycobacterium genome led to the development of two IGRA variants for TB diagnosis
- IGRA tests detect interferon-gamma release by activated memory T-cells in response to stimulation with recombinant proteins ESAT6 and CFP10
- Fresh blood is required for the analysis
ELISPOT (T-SPOT.TB) System
- Isolates peripheral blood mononuclear cells
- Evaluates the number of stain zones (visual or device-based)
- Sustains peripheral T-cells that release interferon-gamma after incubation with CFRT10 and ESAT6 recombinant proteins
ELISA QuantiFERON System
- Evaluates the concentration of interferon-gamma in blood plasma using enzyme-linked immunosorbent analysis
- Uses whole blood incubated with CFP10 and ESAT6 recombinant proteins
- QuantiFERON Coat TB Golden Tube is used for analysis
Limitations of IGRA Tests
- High cost
- Requires equipped laboratory and specialists
- Strict requirements for biological material preparation, storage, and blood processing
- Intravenous manipulation required in children
- Further studies needed to define their relevance in TB diagnostics
Interferon-Gamma Release Assays (IGRA)
- International studies on the mycobacterium genome led to the development of two IGRA variants for TB diagnosis
- IGRA tests detect interferon-gamma release by activated memory T-cells in response to stimulation with recombinant proteins ESAT6 and CFP10
- Fresh blood is required for the analysis
ELISPOT (T-SPOT.TB) System
- Isolates peripheral blood mononuclear cells
- Evaluates the number of stain zones (visual or device-based)
- Sustains peripheral T-cells that release interferon-gamma after incubation with CFRT10 and ESAT6 recombinant proteins
ELISA QuantiFERON System
- Evaluates the concentration of interferon-gamma in blood plasma using enzyme-linked immunosorbent analysis
- Uses whole blood incubated with CFP10 and ESAT6 recombinant proteins
- QuantiFERON Coat TB Golden Tube is used for analysis
Limitations of IGRA Tests
- High cost
- Requires equipped laboratory and specialists
- Strict requirements for biological material preparation, storage, and blood processing
- Intravenous manipulation required in children
- Further studies needed to define their relevance in TB diagnostics
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Description
This quiz covers the development of diagnostic test systems for detecting Mycobacterium Tuberculosis and Mycobacterium Bovis using Interferon-gamma release assays (IGRA) based on laboratory evaluation of interferon gamma release by activated memory t-cells.
