12 Questions
What is the main purpose of impregnation (infiltration) in tissue processing?
To replace the clearing agent with a medium that fills tissue cavities
Which embedding medium is commonly used for routine tissue processing?
Paraffin wax
When is Celloidin (Collodion) embedding medium mainly used?
For preparing soft tissue sections of mixed consistency
What is the purpose of using Gelatin as an embedding medium?
To prevent fragmentation of tough and friable tissues
What is paraffin wax commonly purchased with for histological use?
~60°C melting point
Why is it advised to use paraffin wax slightly above its melting point in histological processing?
To ensure complete impregnation of tissues
What is the purpose of immersing the specimen in fresh melted paraffin for approximately 3 hours?
To ensure complete removal of the clearing agent from the tissue
What does vacuum embedding under negative atmospheric pressure help reduce during tissue processing?
Heat-induced tissue hardening
How many changes of wax are usually required in automatic tissue processing to properly impregnate the specimen?
1-2
How is paraffin wax traditionally marketed based on its properties?
By its melting points
What is the recommended maximum time for tissues to be left in the paraffin oven to prevent shrinkage and hardening?
Between 4-6 hours
How does vacuum embedding contribute to reducing the time required for complete impregnation of tissues?
By minimizing heat-induced tissue hardening
Test your knowledge on the processes of infiltration (impregnation) and embedding in histology. Learn about the steps involved in replacing the clearing agent with a medium in tissues and placing impregnated tissue in a mold. Find out about different types of infiltrating and embedding techniques.
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