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Infiltration and Embedding Quiz

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Questions and Answers

What is the main purpose of impregnation (infiltration) in tissue processing?

To replace the clearing agent with a medium that fills tissue cavities (correct)

To remove tissue cavities

To freeze the tissue for preservation

To soften the tissue for cutting

Which embedding medium is commonly used for routine tissue processing?

Paraffin wax (correct)

Celloidin (Collodion)

Plastic

Gelatin

When is Celloidin (Collodion) embedding medium mainly used?

For tough and friable tissues

For preparing soft tissue sections of mixed consistency (correct)

For embedding delicate specimens

For frozen tissue sections

What is the purpose of using Gelatin as an embedding medium?

To prevent fragmentation of tough and friable tissues Signup and view all the answers

What is paraffin wax commonly purchased with for histological use?

~60°C melting point Signup and view all the answers

Why is it advised to use paraffin wax slightly above its melting point in histological processing?

To ensure complete impregnation of tissues Signup and view all the answers

What is the purpose of immersing the specimen in fresh melted paraffin for approximately 3 hours?

To ensure complete removal of the clearing agent from the tissue Signup and view all the answers

What does vacuum embedding under negative atmospheric pressure help reduce during tissue processing?

Heat-induced tissue hardening Signup and view all the answers

How many changes of wax are usually required in automatic tissue processing to properly impregnate the specimen?

1-2 Signup and view all the answers

How is paraffin wax traditionally marketed based on its properties?

By its melting points Signup and view all the answers

What is the recommended maximum time for tissues to be left in the paraffin oven to prevent shrinkage and hardening?

Between 4-6 hours Signup and view all the answers

How does vacuum embedding contribute to reducing the time required for complete impregnation of tissues?

By minimizing heat-induced tissue hardening Signup and view all the answers