Podcast
Questions and Answers
What is the main purpose of impregnation (infiltration) in tissue processing?
What is the main purpose of impregnation (infiltration) in tissue processing?
- To replace the clearing agent with a medium that fills tissue cavities (correct)
- To remove tissue cavities
- To freeze the tissue for preservation
- To soften the tissue for cutting
Which embedding medium is commonly used for routine tissue processing?
Which embedding medium is commonly used for routine tissue processing?
- Paraffin wax (correct)
- Celloidin (Collodion)
- Plastic
- Gelatin
When is Celloidin (Collodion) embedding medium mainly used?
When is Celloidin (Collodion) embedding medium mainly used?
- For tough and friable tissues
- For preparing soft tissue sections of mixed consistency (correct)
- For embedding delicate specimens
- For frozen tissue sections
What is the purpose of using Gelatin as an embedding medium?
What is the purpose of using Gelatin as an embedding medium?
What is paraffin wax commonly purchased with for histological use?
What is paraffin wax commonly purchased with for histological use?
Why is it advised to use paraffin wax slightly above its melting point in histological processing?
Why is it advised to use paraffin wax slightly above its melting point in histological processing?
What is the purpose of immersing the specimen in fresh melted paraffin for approximately 3 hours?
What is the purpose of immersing the specimen in fresh melted paraffin for approximately 3 hours?
What does vacuum embedding under negative atmospheric pressure help reduce during tissue processing?
What does vacuum embedding under negative atmospheric pressure help reduce during tissue processing?
How many changes of wax are usually required in automatic tissue processing to properly impregnate the specimen?
How many changes of wax are usually required in automatic tissue processing to properly impregnate the specimen?
How is paraffin wax traditionally marketed based on its properties?
How is paraffin wax traditionally marketed based on its properties?
What is the recommended maximum time for tissues to be left in the paraffin oven to prevent shrinkage and hardening?
What is the recommended maximum time for tissues to be left in the paraffin oven to prevent shrinkage and hardening?
How does vacuum embedding contribute to reducing the time required for complete impregnation of tissues?
How does vacuum embedding contribute to reducing the time required for complete impregnation of tissues?
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