Podcast
Questions and Answers
What is the condition required for ppt or agglutination to occur?
What is the condition required for ppt or agglutination to occur?
What happens when there is excess antigen?
What happens when there is excess antigen?
Why does excess antibody prevent cross-linking?
Why does excess antibody prevent cross-linking?
What is the importance of the correct ratio of antibody to antigen?
What is the importance of the correct ratio of antibody to antigen?
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What happens when there is no cross-linking?
What happens when there is no cross-linking?
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What is the result of excess antigen in an agglutination reaction?
What is the result of excess antigen in an agglutination reaction?
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Why is it essential to have the correct ratio of antibody to antigen?
Why is it essential to have the correct ratio of antibody to antigen?
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What is the consequence of excess antibody in an agglutination reaction?
What is the consequence of excess antibody in an agglutination reaction?
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What is necessary for the occurrence of ppt or agglutination?
What is necessary for the occurrence of ppt or agglutination?
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What is the purpose of determining the correct ratio of antibody to antigen?
What is the purpose of determining the correct ratio of antibody to antigen?
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Study Notes
Agglutination and Antibody-Antigen Ratio
- Agglutination only occurs when there is a correct ratio of antibody to antigen.
- This ratio is crucial for determining the amount of antigen present.
Effect of Excess Antigen
- Excess antigen prevents agglutination by saturating all available antibody sites.
- This prevents cross-linking between antibodies, as all sites are occupied.
Effect of Excess Antibody
- Excess antibody prevents cross-linking by providing enough antibodies to bind to each individual antigen.
- This makes it statistically unlikely for two antibodies to share an antigen, resulting in no cross-linking.
Agglutination and Antibody-Antigen Ratio
- Agglutination only occurs when there is a correct ratio of antibody to antigen.
- This ratio is crucial for determining the amount of antigen present.
Effect of Excess Antigen
- Excess antigen prevents agglutination by saturating all available antibody sites.
- This prevents cross-linking between antibodies, as all sites are occupied.
Effect of Excess Antibody
- Excess antibody prevents cross-linking by providing enough antibodies to bind to each individual antigen.
- This makes it statistically unlikely for two antibodies to share an antigen, resulting in no cross-linking.
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Description
Learn about the importance of the correct ratio of antibody to antigen in immunology, and how it affects agglutination reactions. Discover why excess antigen or antibody hinders cross-linking and affects the detection of antigens.
