10 Questions
What is the condition required for ppt or agglutination to occur?
Correct ratio of antibody to antigen
What happens when there is excess antigen?
Antigens bind to all available antibody sites
Why does excess antibody prevent cross-linking?
Because enough antibody binds to each individual antigen
What is the importance of the correct ratio of antibody to antigen?
It determines how much antigen is present
What happens when there is no cross-linking?
No ppt or agglutination occurs
What is the result of excess antigen in an agglutination reaction?
Binding of all available antibody sites
Why is it essential to have the correct ratio of antibody to antigen?
To facilitate cross-linking and agglutination
What is the consequence of excess antibody in an agglutination reaction?
Prevention of cross-linking
What is necessary for the occurrence of ppt or agglutination?
Correct ratio of antibody to antigen
What is the purpose of determining the correct ratio of antibody to antigen?
To determine the amount of antigen present
Study Notes
Agglutination and Antibody-Antigen Ratio
- Agglutination only occurs when there is a correct ratio of antibody to antigen.
- This ratio is crucial for determining the amount of antigen present.
Effect of Excess Antigen
- Excess antigen prevents agglutination by saturating all available antibody sites.
- This prevents cross-linking between antibodies, as all sites are occupied.
Effect of Excess Antibody
- Excess antibody prevents cross-linking by providing enough antibodies to bind to each individual antigen.
- This makes it statistically unlikely for two antibodies to share an antigen, resulting in no cross-linking.
Agglutination and Antibody-Antigen Ratio
- Agglutination only occurs when there is a correct ratio of antibody to antigen.
- This ratio is crucial for determining the amount of antigen present.
Effect of Excess Antigen
- Excess antigen prevents agglutination by saturating all available antibody sites.
- This prevents cross-linking between antibodies, as all sites are occupied.
Effect of Excess Antibody
- Excess antibody prevents cross-linking by providing enough antibodies to bind to each individual antigen.
- This makes it statistically unlikely for two antibodies to share an antigen, resulting in no cross-linking.
Learn about the importance of the correct ratio of antibody to antigen in immunology, and how it affects agglutination reactions. Discover why excess antigen or antibody hinders cross-linking and affects the detection of antigens.
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