11 Questions
What is the difference in charge density between the inner and outer layers of the ionic cloud surrounding red blood cells in an electrolyte solution?
The inner layer has a higher charge density than the outer layer.
What is the term used to describe the potential difference between the stationary layer of fluid attached to the red blood cell surface and the surrounding electrolyte solution?
Zeta potential
What is the purpose of the $ ext{slide method}$ and $ ext{tube method}$ in testing for hemagglutination?
To visually observe the clumping of red blood cells.
What is the purpose of the $ ext{gel method}$ in testing for hemagglutination?
To visually observe the formation of a solid band of agglutinated red blood cells at the top of the gel column.
What is a potential cause of an abnormality in the zeta potential of red blood cells?
Changes in the charge density of the ionic cloud surrounding the red blood cells.
Why is it essential to observe agglutination reactions microscopically?
To differentiate pseudoagglutination (rouleaux) from true agglutination
What is the significance of RBCs forming a well-delineated pellet at the bottom of the microtube in the tube method?
It confirms the absence of hemolysis in the sample
What does it mean when numerous small clumps of cells exist amid a sea of free cells in an agglutination reaction?
Mixed field reactions
In the gel column, where are RBC agglutinates predominantly seen when hemolysis occurs?
Lower half of the gel column
What does a turbid background with small agglutinates indicate in an agglutination reaction?
Presence of grade 2 agglutination
Why do we use distilled water during some test procedures for RBCs?
To observe the absence of agglutinates
Explore the zeta potential and charge characteristics of red blood cells, and understand the differences in charge density within the Ionic cloud surrounding the cells. Learn about common immunohematologic reactions like hemagglutination and the implications of abnormalities in zeta potential.
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