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Fluorescence is the property of certain molecules to absorb light at a longer wavelength and emit light at a shorter wavelength when it is illuminated by light of a different wavelength.
Fluorescence is the property of certain molecules to absorb light at a longer wavelength and emit light at a shorter wavelength when it is illuminated by light of a different wavelength.
False
Immunofluorescence is a technique used to visualize specific proteins or antigens in tissue sections by binding a non-specific antibody chemically conjugated with a fluorescent dye.
Immunofluorescence is a technique used to visualize specific proteins or antigens in tissue sections by binding a non-specific antibody chemically conjugated with a fluorescent dye.
False
The emitted light in fluorescence can be viewed with a normal light microscope.
The emitted light in fluorescence can be viewed with a normal light microscope.
False
Immunofluorescence can be used for the detection of antigens directed against antibodies already known to be present in a given tissue section or cell preparation.
Immunofluorescence can be used for the detection of antigens directed against antibodies already known to be present in a given tissue section or cell preparation.
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Fluorescein isothiocyanate (FITC) is a fluorescent dye that makes antibodies glow red microscopically under ultraviolet light.
Fluorescein isothiocyanate (FITC) is a fluorescent dye that makes antibodies glow red microscopically under ultraviolet light.
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Immunofluorescence can be used to stain cell membrane molecules or tissue sections.
Immunofluorescence can be used to stain cell membrane molecules or tissue sections.
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Direct immunofluorescence is a double-layer technique.
Direct immunofluorescence is a double-layer technique.
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In indirect immunofluorescence, a fluorochrome-conjugated anti-immunoglobulin serum is applied directly to the tissue substrate.
In indirect immunofluorescence, a fluorochrome-conjugated anti-immunoglobulin serum is applied directly to the tissue substrate.
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Indirect immunofluorescence is used to detect the presence of autoantibodies in a patient's serum.
Indirect immunofluorescence is used to detect the presence of autoantibodies in a patient's serum.
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Fluorescein is used to conjugate the antibody in indirect immunofluorescence.
Fluorescein is used to conjugate the antibody in indirect immunofluorescence.
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Direct immunofluorescence is used to detect the distribution of a thyroid autoantigen.
Direct immunofluorescence is used to detect the distribution of a thyroid autoantigen.
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In direct immunofluorescence, the site where the antibody attaches will show red fluorescence.
In direct immunofluorescence, the site where the antibody attaches will show red fluorescence.
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The fluorescence is brighter in the direct test than in the indirect test.
The fluorescence is brighter in the direct test than in the indirect test.
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The primary antibody needs to be conjugated with a fluorochrome in the indirect immunofluorescence technique.
The primary antibody needs to be conjugated with a fluorochrome in the indirect immunofluorescence technique.
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Immunofluorescence is only used for identifying bacterial species.
Immunofluorescence is only used for identifying bacterial species.
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Autoimmune diseases are characterized by antibodies directed against non-self-antigens.
Autoimmune diseases are characterized by antibodies directed against non-self-antigens.
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Fixation is used to revive the cell's functions.
Fixation is used to revive the cell's functions.
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Immunofluorescence is not suitable for detecting autoantibodies.
Immunofluorescence is not suitable for detecting autoantibodies.
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