Podcast
Questions and Answers
What is the primary purpose of the Immunofluorescence Testing method?
What is the primary purpose of the Immunofluorescence Testing method?
- To quantify enzyme activity in biological samples
- To identify specific receptors in cells using a fluorescently labeled antibody (correct)
- To measure the concentration of antigens in a serum sample
- To determine the type of pathogen causing an infection
In which step of the ELISA process is the unknown serum added?
In which step of the ELISA process is the unknown serum added?
- Immediately after attaching the known antigen to the well (correct)
- Before the antigen is attached to the well
- After the substrate for the enzyme is added
- Before rinsing the well for the second time
What does the intensity of the color developed in an ELISA test indicate?
What does the intensity of the color developed in an ELISA test indicate?
- The presence of antigens from pathogens
- The purity of the serum sample
- The amount of unknown antibody present in the sample (correct)
- The presence of fluorescent antibodies
Which of the following conditions is NOT commonly screened using Indirect ELISA?
Which of the following conditions is NOT commonly screened using Indirect ELISA?
During the ELISA process, what is the purpose of rinsing the well?
During the ELISA process, what is the purpose of rinsing the well?
What is the primary purpose of epinephrine in treating anaphylactic attacks?
What is the primary purpose of epinephrine in treating anaphylactic attacks?
Which blood type is considered the universal donor and why?
Which blood type is considered the universal donor and why?
What genetic combinations lead to an individual having type A blood?
What genetic combinations lead to an individual having type A blood?
What defines the Rh factor in terms of blood type inheritance?
What defines the Rh factor in terms of blood type inheritance?
What is the mechanism by which allergy 'vaccines' aim to reduce allergic reactions?
What is the mechanism by which allergy 'vaccines' aim to reduce allergic reactions?
What is the primary difference between an Arthus reaction and serum sickness?
What is the primary difference between an Arthus reaction and serum sickness?
Which of the following treatments can prevent hemolytic disease of the newborn?
Which of the following treatments can prevent hemolytic disease of the newborn?
What is the most severe consequence of receiving the wrong blood type during a transfusion?
What is the most severe consequence of receiving the wrong blood type during a transfusion?
Why are universal recipients considered type AB blood?
Why are universal recipients considered type AB blood?
Which of the following options is a characteristic of asthma?
Which of the following options is a characteristic of asthma?
What are common reactions associated with drug allergies?
What are common reactions associated with drug allergies?
What symptoms are often associated with food allergies?
What symptoms are often associated with food allergies?
How is specific allergen identification important in diagnosing allergies?
How is specific allergen identification important in diagnosing allergies?
What is a significant drawback of food allergy skin tests?
What is a significant drawback of food allergy skin tests?
What is the role of the RAST test in allergy diagnosis?
What is the role of the RAST test in allergy diagnosis?
What kind of allergy is characterized by life-threatening systemic reactions?
What kind of allergy is characterized by life-threatening systemic reactions?
What is the primary purpose of allergy treatment drugs?
What is the primary purpose of allergy treatment drugs?
Where are allergen extracts typically applied for skin testing?
Where are allergen extracts typically applied for skin testing?
Which of the following describes hay fever?
Which of the following describes hay fever?
What is a typical symptom of the Arthus reaction?
What is a typical symptom of the Arthus reaction?
What primarily causes the symptoms in an Arthus reaction?
What primarily causes the symptoms in an Arthus reaction?
In type IV hypersensitivity reactions, which cell type is primarily involved?
In type IV hypersensitivity reactions, which cell type is primarily involved?
What is the time frame for symptoms to arise in type IV hypersensitivity reactions?
What is the time frame for symptoms to arise in type IV hypersensitivity reactions?
What is a potential trigger for serum sickness?
What is a potential trigger for serum sickness?
Which of the following conditions is associated with delayed type hypersensitivity?
Which of the following conditions is associated with delayed type hypersensitivity?
What is the primary cause of contact dermatitis?
What is the primary cause of contact dermatitis?
What can lead to necrosis in an Arthus reaction?
What can lead to necrosis in an Arthus reaction?
What is the primary characteristic of allergens?
What is the primary characteristic of allergens?
Which type of hypersensitivity is characterized by an immediate allergic reaction?
Which type of hypersensitivity is characterized by an immediate allergic reaction?
How does the Hygiene Hypothesis explain the rise of allergies?
How does the Hygiene Hypothesis explain the rise of allergies?
What role do mast cells play in the allergic response?
What role do mast cells play in the allergic response?
What is a possible physiological effect of the chemical mediators released during an allergic reaction?
What is a possible physiological effect of the chemical mediators released during an allergic reaction?
What distinguishes exogenous antigens from endogenous antigens in the context of hypersensitivity?
What distinguishes exogenous antigens from endogenous antigens in the context of hypersensitivity?
Which cytokine is NOT commonly released during an allergic response?
Which cytokine is NOT commonly released during an allergic response?
What commonly triggers the degranulation of mast cells during an allergic reaction?
What commonly triggers the degranulation of mast cells during an allergic reaction?
Which of these examples is classified as an ingestant allergen?
Which of these examples is classified as an ingestant allergen?
What impact do larger families have on the development of allergies, according to the hygiene hypothesis?
What impact do larger families have on the development of allergies, according to the hygiene hypothesis?
What is the primary function of lymph fluid in the immune system?
What is the primary function of lymph fluid in the immune system?
What differentiates B cells from T cells in terms of their maturation?
What differentiates B cells from T cells in terms of their maturation?
What role do plasma cells play in the immune response?
What role do plasma cells play in the immune response?
How do T cells achieve self-tolerance during their maturation process?
How do T cells achieve self-tolerance during their maturation process?
What type of immune response is described when multiple antibodies are produced against different features of an antigen?
What type of immune response is described when multiple antibodies are produced against different features of an antigen?
Which type of T cell is primarily responsible for directly destroying infected or cancerous cells?
Which type of T cell is primarily responsible for directly destroying infected or cancerous cells?
Which cytokine is known for its role in modulating smooth muscle responses?
Which cytokine is known for its role in modulating smooth muscle responses?
What is the main feature of an antigen that can trigger distinct antibody production?
What is the main feature of an antigen that can trigger distinct antibody production?
What is one primary function of cytokines in the immune system?
What is one primary function of cytokines in the immune system?
What happens to T cells that survive the selection process in the thymus?
What happens to T cells that survive the selection process in the thymus?
Flashcards
Immunofluorescence Technique
Immunofluorescence Technique
Uses fluorescently labeled antibodies to detect specific receptors on cells. Cells containing matching receptors fluoresce when illuminated with UV light.
ELISA Procedure (Steps)
ELISA Procedure (Steps)
Enzyme-linked Immunosorbent Assay. Antigens, unknown antibodies, enzyme-linked antibodies are used to detect presence & amount of unknown antibody. Color change signals presence.
ELISA Detection
ELISA Detection
ELISA detects antibodies or antigens (such as drugs).
Indirect ELISA
Indirect ELISA
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ELISA Color Development
ELISA Color Development
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Atopic Diseases
Atopic Diseases
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Hay Fever
Hay Fever
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Asthma
Asthma
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Eczema
Eczema
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Food Allergy
Food Allergy
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Drug Allergy
Drug Allergy
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Anaphylaxis
Anaphylaxis
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Skin Testing (Allergy)
Skin Testing (Allergy)
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Allergy Mimics
Allergy Mimics
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Radioallergosorbent (RAST) Test
Radioallergosorbent (RAST) Test
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Epinephrine's function in allergies
Epinephrine's function in allergies
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Desensitization treatment for allergies
Desensitization treatment for allergies
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ABO blood types
ABO blood types
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Universal blood donor
Universal blood donor
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Universal blood recipient
Universal blood recipient
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Rh factor
Rh factor
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Hemolytic disease of the newborn
Hemolytic disease of the newborn
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Type III hypersensitivity
Type III hypersensitivity
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Serum sickness
Serum sickness
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Arthus reaction
Arthus reaction
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Immunopathology
Immunopathology
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Allergy
Allergy
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Hyposensitivity
Hyposensitivity
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Hygiene Hypothesis
Hygiene Hypothesis
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Allergens
Allergens
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Mast Cells
Mast Cells
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Degranulation
Degranulation
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Histamine
Histamine
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Cytokines
Cytokines
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Type I Allergic Reactions
Type I Allergic Reactions
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Infectious Allergy
Infectious Allergy
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Lymphatic System
Lymphatic System
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Lymph Fluid
Lymph Fluid
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B Cells
B Cells
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Plasma Cells
Plasma Cells
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Antigen
Antigen
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Helper T Cells
Helper T Cells
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Cytotoxic T Cells
Cytotoxic T Cells
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Humoral Immune Response
Humoral Immune Response
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