Immunology: ELISA Methods Quiz

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Questions and Answers

What is the primary purpose of the Immunofluorescence Testing method?

  • To quantify enzyme activity in biological samples
  • To identify specific receptors in cells using a fluorescently labeled antibody (correct)
  • To measure the concentration of antigens in a serum sample
  • To determine the type of pathogen causing an infection

In which step of the ELISA process is the unknown serum added?

  • Immediately after attaching the known antigen to the well (correct)
  • Before the antigen is attached to the well
  • After the substrate for the enzyme is added
  • Before rinsing the well for the second time

What does the intensity of the color developed in an ELISA test indicate?

  • The presence of antigens from pathogens
  • The purity of the serum sample
  • The amount of unknown antibody present in the sample (correct)
  • The presence of fluorescent antibodies

Which of the following conditions is NOT commonly screened using Indirect ELISA?

<p>Influenza (D)</p> Signup and view all the answers

During the ELISA process, what is the purpose of rinsing the well?

<p>To remove any unbound antibodies and ensure only specific reactions are measured (D)</p> Signup and view all the answers

What is the primary purpose of epinephrine in treating anaphylactic attacks?

<p>To reverse airway constriction (D)</p> Signup and view all the answers

Which blood type is considered the universal donor and why?

<p>Type O, because it lacks A and B antigens (D)</p> Signup and view all the answers

What genetic combinations lead to an individual having type A blood?

<p>AA or AO genotype (A)</p> Signup and view all the answers

What defines the Rh factor in terms of blood type inheritance?

<p>Rh+ is dominant over Rh- (B)</p> Signup and view all the answers

What is the mechanism by which allergy 'vaccines' aim to reduce allergic reactions?

<p>By creating IgG blocking antibodies (A)</p> Signup and view all the answers

What is the primary difference between an Arthus reaction and serum sickness?

<p>Arthus reaction occurs immediately while serum sickness has delayed symptoms (B)</p> Signup and view all the answers

Which of the following treatments can prevent hemolytic disease of the newborn?

<p>RhoGAM injections (D)</p> Signup and view all the answers

What is the most severe consequence of receiving the wrong blood type during a transfusion?

<p>Massive hemolysis and systemic shock (B)</p> Signup and view all the answers

Why are universal recipients considered type AB blood?

<p>It has no antibodies to A or B antigens (B)</p> Signup and view all the answers

Which of the following options is a characteristic of asthma?

<p>Episodes of breathing difficulties due to severe bronchoconstriction (B)</p> Signup and view all the answers

What are common reactions associated with drug allergies?

<p>Range from mild rash to fatal anaphylaxis (B)</p> Signup and view all the answers

What symptoms are often associated with food allergies?

<p>Vomiting, diarrhea, and abdominal pain (A)</p> Signup and view all the answers

How is specific allergen identification important in diagnosing allergies?

<p>It allows tailored treatment options (B)</p> Signup and view all the answers

What is a significant drawback of food allergy skin tests?

<p>They can provide false negatives (C)</p> Signup and view all the answers

What is the role of the RAST test in allergy diagnosis?

<p>It measures levels of IgE to specific allergens (A)</p> Signup and view all the answers

What kind of allergy is characterized by life-threatening systemic reactions?

<p>Anaphylaxis (C)</p> Signup and view all the answers

What is the primary purpose of allergy treatment drugs?

<p>To block the action of lymphocytes and mast cells (D)</p> Signup and view all the answers

Where are allergen extracts typically applied for skin testing?

<p>On the inner forearms or back (C)</p> Signup and view all the answers

Which of the following describes hay fever?

<p>A seasonal reaction to inhaled plant pollen or molds (D)</p> Signup and view all the answers

What is a typical symptom of the Arthus reaction?

<p>Redness and swelling at the injection site (D)</p> Signup and view all the answers

What primarily causes the symptoms in an Arthus reaction?

<p>Histamine release and tissue destruction (A)</p> Signup and view all the answers

In type IV hypersensitivity reactions, which cell type is primarily involved?

<p>T cells (C)</p> Signup and view all the answers

What is the time frame for symptoms to arise in type IV hypersensitivity reactions?

<p>One to several days after exposure (B)</p> Signup and view all the answers

What is a potential trigger for serum sickness?

<p>Injections of animal sera or hormones (C)</p> Signup and view all the answers

Which of the following conditions is associated with delayed type hypersensitivity?

<p>Tuberculosis (D)</p> Signup and view all the answers

What is the primary cause of contact dermatitis?

<p>Exposure to irritants or allergens (C)</p> Signup and view all the answers

What can lead to necrosis in an Arthus reaction?

<p>Intravascular blood clotting (D)</p> Signup and view all the answers

What is the primary characteristic of allergens?

<p>They are antigens that only affect allergic individuals. (A)</p> Signup and view all the answers

Which type of hypersensitivity is characterized by an immediate allergic reaction?

<p>Type I hypersensitivity. (B)</p> Signup and view all the answers

How does the Hygiene Hypothesis explain the rise of allergies?

<p>Lack of early immune stimulus due to reduced microbial exposure. (D)</p> Signup and view all the answers

What role do mast cells play in the allergic response?

<p>They release chemical mediators upon re-exposure to an allergen. (B)</p> Signup and view all the answers

What is a possible physiological effect of the chemical mediators released during an allergic reaction?

<p>Altered blood pressure and respiration. (B)</p> Signup and view all the answers

What distinguishes exogenous antigens from endogenous antigens in the context of hypersensitivity?

<p>Exogenous antigens include microbes and pollen, while endogenous are self-derived. (D)</p> Signup and view all the answers

Which cytokine is NOT commonly released during an allergic response?

<p>Cortisol. (B)</p> Signup and view all the answers

What commonly triggers the degranulation of mast cells during an allergic reaction?

<p>Binding of allergen molecules to IgE on mast cells. (C)</p> Signup and view all the answers

Which of these examples is classified as an ingestant allergen?

<p>Peanuts. (A)</p> Signup and view all the answers

What impact do larger families have on the development of allergies, according to the hygiene hypothesis?

<p>They reduce the odds of hay fever and asthma. (D)</p> Signup and view all the answers

What is the primary function of lymph fluid in the immune system?

<p>To carry White Blood Cells and provide immune support (C)</p> Signup and view all the answers

What differentiates B cells from T cells in terms of their maturation?

<p>B cells originate in the bone marrow and mature there, T cells originate in the bone marrow but mature in the thymus (D)</p> Signup and view all the answers

What role do plasma cells play in the immune response?

<p>They produce antibodies that recognize specific antigens (B)</p> Signup and view all the answers

How do T cells achieve self-tolerance during their maturation process?

<p>They undergo apoptosis if they bind to self-antigens (D)</p> Signup and view all the answers

What type of immune response is described when multiple antibodies are produced against different features of an antigen?

<p>Humoral immune response: polyclonal (D)</p> Signup and view all the answers

Which type of T cell is primarily responsible for directly destroying infected or cancerous cells?

<p>Cytotoxic T cells (D)</p> Signup and view all the answers

Which cytokine is known for its role in modulating smooth muscle responses?

<p>Histamine (A)</p> Signup and view all the answers

What is the main feature of an antigen that can trigger distinct antibody production?

<p>The presence of multiple antigenic determinants (epitopes) (A)</p> Signup and view all the answers

What is one primary function of cytokines in the immune system?

<p>To serve as chemical messengers regulating immune functions (D)</p> Signup and view all the answers

What happens to T cells that survive the selection process in the thymus?

<p>They mature and become functional T cells (C)</p> Signup and view all the answers

Flashcards

Immunofluorescence Technique

Uses fluorescently labeled antibodies to detect specific receptors on cells. Cells containing matching receptors fluoresce when illuminated with UV light.

ELISA Procedure (Steps)

Enzyme-linked Immunosorbent Assay. Antigens, unknown antibodies, enzyme-linked antibodies are used to detect presence & amount of unknown antibody. Color change signals presence.

ELISA Detection

ELISA detects antibodies or antigens (such as drugs).

Indirect ELISA

A type of ELISA used to screen for antibodies related to specific conditions like HIV, Hepatitis A & C, and Cholera.

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ELISA Color Development

Color change indicates the presence of the targeted antibody; stronger color = more antibody.

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Atopic Diseases

Conditions where the immune system overreacts to harmless substances, causing symptoms like inflammation and itching.

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Hay Fever

Seasonal allergy caused by inhaled plant pollen or molds.

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Asthma

Breathing difficulty due to bronchoconstriction, often triggered by allergens or infections.

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Eczema

Skin condition with dry, scaly, thickened skin, possibly caused by various allergens.

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Food Allergy

Immune response triggered by food, often leading to digestive or skin issues.

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Drug Allergy

Allergic reaction to medications, ranging from mild to life-threatening.

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Anaphylaxis

Serious, life-threatening allergic reaction affecting the respiratory and circulatory systems.

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Skin Testing (Allergy)

In vivo method using allergens to pinpoint allergic triggers on the skin.

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Allergy Mimics

Conditions that can have symptoms similar to allergies, requiring careful diagnosis.

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Radioallergosorbent (RAST) Test

Laboratory test measuring the amount of IgE antibodies to specific allergens.

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Epinephrine's function in allergies

Epinephrine, also known as adrenaline, is used to treat anaphylactic shock. It reverses airway constriction and slows the release of allergic mediators, buying time for further treatment.

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Desensitization treatment for allergies

Desensitization, or hyposensitization, is a process of controlled injections of specific allergens to reduce allergic reactions. It works by preventing reactions between allergens, IgE antibodies, and mast cells.

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ABO blood types

ABO blood types are based on genetically determined glycoprotein markers (antigens) on red blood cells. They are inherited as A, B, or O alleles.

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Universal blood donor

Type O blood is the universal donor because it lacks both A and B antigens, preventing agglutination with other blood types.

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Universal blood recipient

Type AB blood lacks antibodies to A and B antigens, making it the universal recipient.

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Rh factor

Rh factor, specifically the D antigen, is another blood type marker. Rh+ is dominant, while Rh- is recessive.

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Hemolytic disease of the newborn

This condition results from Rh incompatibility between mother and fetus, potentially harming the baby.

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Type III hypersensitivity

Type III hypersensitivity involves immune complex reactions, caused by antigen-antibody complexes in the blood.

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Serum sickness

A systemic reaction involving antigen-antibody complexes throughout the body, causing inflammation.

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Arthus reaction

A localized reaction caused by immune complexes forming at the site of injection.

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Immunopathology

The study of diseases caused by over- or under-reactions of the immune system.

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Allergy

An exaggerated immune response that causes inflammation, triggered by specific antigens called allergens.

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Hyposensitivity

A weakened or impaired immune system.

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Hygiene Hypothesis

The idea that early exposure to microbes, especially in childhood, helps train the immune system and reduce allergies.

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Allergens

Substances that trigger an allergic reaction in sensitive individuals.

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Mast Cells

Immune cells found in tissues that release chemicals like histamine during allergic reactions.

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Degranulation

The process where mast cells release their granules containing inflammatory chemicals.

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Histamine

A chemical mediator released by mast cells that causes many of the classic symptoms of allergies, like sneezing, itching, and runny nose.

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Cytokines

Chemical messengers released by cells, particularly during immune responses.

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Type I Allergic Reactions

Immediate hypersensitivity reactions triggered by allergens, often involving IgE antibodies and mast cells.

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Infectious Allergy

A type of delayed-type hypersensitivity that occurs when someone previously exposed to an infection is re-exposed to the same pathogen's antigens, leading to an inflammatory reaction.

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Lymphatic System

A network of vessels that transport lymph fluid, containing white blood cells, to lymph nodes. It plays a crucial role in the immune system by facilitating the circulation of immune cells.

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Lymph Fluid

A fluid that circulates throughout the lymphatic system, carrying white blood cells, including lymphocytes (B cells and T cells), and macrophages.

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B Cells

White blood cells that originate and mature in the bone marrow. They produce antibodies that specifically target antigens.

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Plasma Cells

Specialized B cells that produce and secrete large amounts of antibodies.

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Antigen

A molecule, usually a protein or carbohydrate, that triggers an immune response. They have specific regions called epitopes that bind to antibodies.

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Helper T Cells

A type of T cell that helps activate other immune cells, like B cells and cytotoxic T cells, to fight infections.

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Cytotoxic T Cells

A type of T cell that directly kills infected cells or cancer cells.

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Humoral Immune Response

An immune response mediated by antibodies produced by B cells. This response is effective against pathogens in the blood and other body fluids.

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