Immunoassays
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Questions and Answers

Which type of molecule is often detected by immunoassays?

  • Nucleic acids
  • Proteins only
  • Proteins and small molecules (correct)
  • Small molecules only
  • What are multi-step immunoassays often called?

  • Homogeneous immunoassays
  • Non-separation immunoassays
  • Heterogeneous immunoassays (correct)
  • Separation immunoassays
  • What is the purpose of using calibrators in immunoassays?

  • To develop proper antibodies
  • To interpret the signal strength (correct)
  • To measure the concentration of analyte
  • To wash away or separate reagents
  • Which enzyme is commonly used in enzyme immunoassays (EIAs)?

    <p>Horseradish peroxidase (HRP)</p> Signup and view all the answers

    What is the newer approach to immunoassays that combines real-time quantitative polymerase chain reaction (RT qPCR) and traditional immunoassay techniques called?

    <p>Real-time immunoquantitative PCR (iqPCR)</p> Signup and view all the answers

    Which type of immunoassay uses radioactive isotopes as labels?

    <p>Radioimmunoassay (RIA)</p> Signup and view all the answers

    What is a label-free immunoassay that can detect binding between an unlabeled antibody and antigens?

    <p>Surface plasmon resonance (SPR)</p> Signup and view all the answers

    Which immunoassay technique measures the fluorescence polarization signal after incubation, without separating bound and free labels?

    <p>Competitive, homogeneous immunoassay</p> Signup and view all the answers

    In which immunoassay technique does the signal intensity decrease as the analyte concentration increases?

    <p>Competitive, homogeneous immunoassay</p> Signup and view all the answers

    Which immunoassay technique uses enzyme-labeled analyte analog molecules that compete with the analyte being tested?

    <p>Competitive, homogeneous immunoassay</p> Signup and view all the answers

    In which immunoassay technique are free antibodies used to bind to drug microparticle conjugates and form aggregates?

    <p>Competitive, heterogeneous immunoassay</p> Signup and view all the answers

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