11 Questions
Which type of molecule is often detected by immunoassays?
Proteins and small molecules
What are multi-step immunoassays often called?
Heterogeneous immunoassays
What is the purpose of using calibrators in immunoassays?
To interpret the signal strength
Which enzyme is commonly used in enzyme immunoassays (EIAs)?
Horseradish peroxidase (HRP)
What is the newer approach to immunoassays that combines real-time quantitative polymerase chain reaction (RT qPCR) and traditional immunoassay techniques called?
Real-time immunoquantitative PCR (iqPCR)
Which type of immunoassay uses radioactive isotopes as labels?
Radioimmunoassay (RIA)
What is a label-free immunoassay that can detect binding between an unlabeled antibody and antigens?
Surface plasmon resonance (SPR)
Which immunoassay technique measures the fluorescence polarization signal after incubation, without separating bound and free labels?
Competitive, homogeneous immunoassay
In which immunoassay technique does the signal intensity decrease as the analyte concentration increases?
Competitive, homogeneous immunoassay
Which immunoassay technique uses enzyme-labeled analyte analog molecules that compete with the analyte being tested?
Competitive, homogeneous immunoassay
In which immunoassay technique are free antibodies used to bind to drug microparticle conjugates and form aggregates?
Competitive, heterogeneous immunoassay
Test your knowledge on immunoassays - the biochemical tests used to measure the presence or concentration of molecules in a solution. This quiz covers the basics of immunoassays, including the use of antibodies and antigens, and the different types of molecules that can be detected.
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