Immunoassays
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Questions and Answers

Which type of molecule is often detected by immunoassays?

  • Nucleic acids
  • Proteins only
  • Proteins and small molecules (correct)
  • Small molecules only

What are multi-step immunoassays often called?

  • Homogeneous immunoassays
  • Non-separation immunoassays
  • Heterogeneous immunoassays (correct)
  • Separation immunoassays

What is the purpose of using calibrators in immunoassays?

  • To develop proper antibodies
  • To interpret the signal strength (correct)
  • To measure the concentration of analyte
  • To wash away or separate reagents

Which enzyme is commonly used in enzyme immunoassays (EIAs)?

<p>Horseradish peroxidase (HRP) (A)</p> Signup and view all the answers

What is the newer approach to immunoassays that combines real-time quantitative polymerase chain reaction (RT qPCR) and traditional immunoassay techniques called?

<p>Real-time immunoquantitative PCR (iqPCR) (A)</p> Signup and view all the answers

Which type of immunoassay uses radioactive isotopes as labels?

<p>Radioimmunoassay (RIA) (B)</p> Signup and view all the answers

What is a label-free immunoassay that can detect binding between an unlabeled antibody and antigens?

<p>Surface plasmon resonance (SPR) (C)</p> Signup and view all the answers

Which immunoassay technique measures the fluorescence polarization signal after incubation, without separating bound and free labels?

<p>Competitive, homogeneous immunoassay (D)</p> Signup and view all the answers

In which immunoassay technique does the signal intensity decrease as the analyte concentration increases?

<p>Competitive, homogeneous immunoassay (B)</p> Signup and view all the answers

Which immunoassay technique uses enzyme-labeled analyte analog molecules that compete with the analyte being tested?

<p>Competitive, homogeneous immunoassay (B)</p> Signup and view all the answers

In which immunoassay technique are free antibodies used to bind to drug microparticle conjugates and form aggregates?

<p>Competitive, heterogeneous immunoassay (C)</p> Signup and view all the answers

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