Podcast
Questions and Answers
What is the primary method of preventing Hepatitis D virus?
What is the primary method of preventing Hepatitis D virus?
- Avoiding contact with infected individuals
- Prevention of Hepatitis B virus (correct)
- Vaccination against Hepatitis D
- Treatment with PEGylated interferon alpha
What is the most common outcome of acute HDV and HBV infection?
What is the most common outcome of acute HDV and HBV infection?
- Recovery from infection without any complications
- Severe acute hepatitis and Chronic Hepatitis D infection in 80% of the cases (correct)
- Chronic Hepatitis D infection in 20% of the cases
- Development of liver cancer
What is the purpose of Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) in Hepatitis D diagnosis?
What is the purpose of Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) in Hepatitis D diagnosis?
- To monitor any ongoing HDV infection (correct)
- To diagnose co-infection
- To diagnose superinfection
- To detect the total anti-HDV antibodies
What is a rare case of Hepatitis D transmission?
What is a rare case of Hepatitis D transmission?
What is the difference between co-infection and superinfection?
What is the difference between co-infection and superinfection?
What is the role of Enzyme Immunoassay (EIA) in Hepatitis D diagnosis?
What is the role of Enzyme Immunoassay (EIA) in Hepatitis D diagnosis?
What is the treatment for Hepatitis D virus?
What is the treatment for Hepatitis D virus?
What is the primary method of diagnosing Hepatitis D infection?
What is the primary method of diagnosing Hepatitis D infection?
What is the primary purpose of the denaturation step in PCR?
What is the primary purpose of the denaturation step in PCR?
What is the optimal temperature for Taq polymerase activity?
What is the optimal temperature for Taq polymerase activity?
What is the primary function of the primers in PCR?
What is the primary function of the primers in PCR?
How many double-stranded DNA sequences are produced after two cycles of PCR?
How many double-stranded DNA sequences are produced after two cycles of PCR?
What is the purpose of the thermocycler in PCR?
What is the purpose of the thermocycler in PCR?
What occurs during the annealing step of PCR?
What occurs during the annealing step of PCR?
How many cycles of PCR are typically performed?
How many cycles of PCR are typically performed?
What is the temperature used during the annealing step of PCR?
What is the temperature used during the annealing step of PCR?
What is the purpose of autoclaving the 3% agarose solution?
What is the purpose of autoclaving the 3% agarose solution?
Why is Fetal Bovine Serum (FBS) not used as a cell culture medium in the plaque assay?
Why is Fetal Bovine Serum (FBS) not used as a cell culture medium in the plaque assay?
What is the temperature at which the agarose solution is mixed with culture medium?
What is the temperature at which the agarose solution is mixed with culture medium?
How long are the cells incubated with formaldehyde?
How long are the cells incubated with formaldehyde?
What is the purpose of shaking the plates every 15 minutes during the viral adsorption step?
What is the purpose of shaking the plates every 15 minutes during the viral adsorption step?
What is the percentage of agarose in the solution used for the overlay?
What is the percentage of agarose in the solution used for the overlay?
What is the purpose of using crystal violet in the plaque assay?
What is the purpose of using crystal violet in the plaque assay?
How long are the plates incubated at 37°C to allow viral plaques to form?
How long are the plates incubated at 37°C to allow viral plaques to form?
What is the primary limitation of Western Blot as a diagnostic tool for HIV infection?
What is the primary limitation of Western Blot as a diagnostic tool for HIV infection?
What is the purpose of Sodium Dodecyl sulphate (SDS) in Western Blot?
What is the purpose of Sodium Dodecyl sulphate (SDS) in Western Blot?
What is the primary function of electrophoresis in Western Blot?
What is the primary function of electrophoresis in Western Blot?
What is the ultimate goal of Western Blot?
What is the ultimate goal of Western Blot?
What is the role of antibodies in Western Blot?
What is the role of antibodies in Western Blot?
What is the name of the gel used in Western Blot?
What is the name of the gel used in Western Blot?
What is the first step in the Western Blot process?
What is the first step in the Western Blot process?
What is the purpose of Western Blot in the context of HIV diagnosis?
What is the purpose of Western Blot in the context of HIV diagnosis?
What is the primary purpose of rinsing the microtiter plate well after immobilizing the antibody?
What is the primary purpose of rinsing the microtiter plate well after immobilizing the antibody?
In a direct ELISA, what binds to the enzyme-linked antibody?
In a direct ELISA, what binds to the enzyme-linked antibody?
What is the purpose of adding a colorless substrate in an ELISA?
What is the purpose of adding a colorless substrate in an ELISA?
What is the purpose of immobilizing the antigen in an indirect ELISA?
What is the purpose of immobilizing the antigen in an indirect ELISA?
What is the role of the secondary antibody in an indirect ELISA?
What is the role of the secondary antibody in an indirect ELISA?
What is the primary difference between a direct and indirect ELISA?
What is the primary difference between a direct and indirect ELISA?
What is the purpose of washing away unbound antigens in an indirect ELISA?
What is the purpose of washing away unbound antigens in an indirect ELISA?
What is the final result of a positive ELISA?
What is the final result of a positive ELISA?
