13 Questions
What is the purpose of the blocking buffer/solution in a Direct ELISA?
To prevent non-specific binding of antibodies to the well surface
In Indirect ELISA, which molecule binds to the primary antibody?
The secondary antibody
What is the role of the capture antibody in Sandwich ELISA?
To bind to the antigen in a specific manner
Why are microplates used for ELISA made of polystyrene?
Polystyrene is hydrophobic, allowing passive adsorption of biomolecules through hydrophobic interactions
What is the function of a mature naive B cell during the initial encounter with a pathogen?
Act as an antigen-presenting cell
What happens to the B cell after a helper T cell specific for the same antigen activates it?
It differentiates into memory B cells and plasma cells
What is the function of memory B cells?
Secrete antibodies to fight against the pathogen
What is the purpose of using an enzyme-linked anti-mouse 2˚ antibody in the given scenario?
To detect different antigens with a single antibody
Which region of the antibody does the enzyme-linked anti-mouse 2˚ antibody bind to?
Constant region
What is the function of the control line in the lateral flow immunoassay?
To indicate if the result is negative or positive
What is the purpose of the chicken IgY antibodies in the lateral flow immunoassay?
To bind to the tagged antibodies in the conjugation pad
Which type of antibodies can be used for research as mentioned in the text?
Monoclonal or polyclonal antibodies
How do antibody-bound antigens reach the test line in the lateral flow immunoassay?
By binding to another antigen-specific antibody on the test line
This quiz covers the method for detecting and quantifying a specific protein in a protein solution using ELISA. It also includes information about the antibody-antigen binding and the non-specific adsorption of the antigen or antibody in Immunosorbent Assays.
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