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Questions and Answers
What is the purpose of the blocking buffer/solution in a Direct ELISA?
In Indirect ELISA, which molecule binds to the primary antibody?
What is the role of the capture antibody in Sandwich ELISA?
Why are microplates used for ELISA made of polystyrene?
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What is the function of a mature naive B cell during the initial encounter with a pathogen?
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What happens to the B cell after a helper T cell specific for the same antigen activates it?
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What is the function of memory B cells?
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What is the purpose of using an enzyme-linked anti-mouse 2˚ antibody in the given scenario?
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Which region of the antibody does the enzyme-linked anti-mouse 2˚ antibody bind to?
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What is the function of the control line in the lateral flow immunoassay?
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What is the purpose of the chicken IgY antibodies in the lateral flow immunoassay?
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Which type of antibodies can be used for research as mentioned in the text?
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How do antibody-bound antigens reach the test line in the lateral flow immunoassay?
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Study Notes
Direct and Indirect ELISA
- Blocking buffer in Direct ELISA prevents non-specific binding of antibodies to the plate.
- In Indirect ELISA, secondary antibodies bind to the primary antibodies enhancing signal detection.
Sandwich ELISA
- The capture antibody attaches to the target antigen, providing specificity and allowing for detection.
Microplates
- Polystyrene microplates are used for ELISA due to their high protein-binding capacity and compatibility with various assays.
Immune Response
- Mature naive B cells recognize and bind to specific antigens during initial encounters with pathogens, beginning the immune response.
- After activation by helper T cells specific to the same antigen, B cells proliferate and differentiate into antibody-secreting plasma cells or memory B cells.
Memory B Cells
- Memory B cells persist long-term after an initial immune response, providing a quicker and stronger response upon subsequent infections.
Antibody Detection
- An enzyme-linked anti-mouse secondary antibody is used to amplify the detection signal in ELISA and binds to the Fc region of the primary antibody.
Lateral Flow Immunoassay
- The control line confirms the validity of the test by showing that the assay worked properly.
- Chicken IgY antibodies serve as a detection agent in lateral flow assays due to their unique properties and reactivity.
Antibody Research
- Different types of antibodies, including polyclonal and monoclonal antibodies, are utilized for research applications.
Antigen Mobility
- Antibody-bound antigens move to the test line in a lateral flow immunoassay through capillary action, allowing for visualization of results.
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Description
This quiz covers the method for detecting and quantifying a specific protein in a protein solution using ELISA. It also includes information about the antibody-antigen binding and the non-specific adsorption of the antigen or antibody in Immunosorbent Assays.