Identification of Gelatin

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10 Questions

What is the percentage difference in weight required to achieve constant weight in the drying process?

0.25%

The drying process is continued until three successive readings match each other.

False

What is the formula used to calculate the percentage of moisture content of the drug?

Loss in weight of the sample x 100 / Weight of the sample

The lemon peel has a strong and ______________ odour.

aromatic

Match the chemical constituents of lemon peel with their descriptions.

Volatile oil = Essential oil Vit.C = Vitamin C Limonene = Terpene Calcium oxalate = Mineral compound Pectin = Carbohydrate Mucilage = Gel-like substance

What is the shape of the lemon peel?

Spiral

The lemon peel has a bitter taste.

True

What is the botanical name of the lemon peel?

Citrus limon

The lemon peel is ______________ in colour on the outer surface.

yellow

What is the thickness of the lemon peel?

2-3 mm

Study Notes

Gelatin

  • Source: Obtained by treating specific animal tissues like skin, tendons, ligaments, and bones of Bos taurus (Bovidae family)
  • Chemical Constituents: Proteins like glutin, mixture of amino acids like glycine, valine, cysteine, glutamic acid, aspartic acid, and tyrosine
  • Gelatinizing agent: Chondrin
  • Adhesive nature: Due to glutin

Macroscopic Features of Gelatin

  • Colour: Yellow or colorless
  • Odour: Odourless
  • Taste: Tasteless

Chemical Tests for Gelatin

  • Ninhydrin test: Forms a red to violet colour on heating with ninhydrin
  • Biuret reaction: Forms a red or violet colour with peptides containing at least two peptide linkages
  • Xanthoproteic reaction: Forms a yellow colour when warmed with concentrated nitric acid, which becomes orange when made alkaline
  • Millon's reaction: Forms a white precipitate that turns red on heating

Uses of Gelatin

  • Nutrient
  • Used in manufacturing suppositories, rubber, printing inks, plastic compounds, artificial silk, photographic plates, film, and matches
  • Used for inhibiting crystallization in bacteriology

Beeswax

  • Aims of pharmacognostic evaluation: Determine purity and quality, identify adulterants or contaminants

Lemon Peel

  • Botanical Origin: Citrus limon (Rutaceae family)
  • Part used: Fresh ripe fruit
  • Chemical Constituents: Volatile oil, Vit.C, limonene, citronellal, alpha pinene, calcium oxalate, pectin, and mucilage
  • Macroscopic Features:
    • Length: 20 cm
    • Width: 1.5 cm
    • Thickness: 2-3 mm
    • Shape: Spiral
    • Colour: Outer surface rough and yellow, inner surface pithy and white
    • Odour: Strong and aromatic
    • Taste: Aromatic and bitter

Microscopic Evaluation of Lemon Peel

  • Prepare microscope and adjust the light
  • Put a pinch of powder drug on a slide

This quiz identifies the protein gelatin through various chemical tests and reactions, including the ninhydrin test, soda lime reaction, tannic acid precipitation, and picric acid test. The uses of gelatin as a nutrient and gelling agent are also discussed.

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