Podcast
Questions and Answers
Which statement is NOT correct regarding new DNA production in a cell before mitosis?
Which statement is NOT correct regarding new DNA production in a cell before mitosis?
- It follows a semi-conservative process.
- Adenine pairs with cytosine during the process. (correct)
- The process is commonly referred to as DNA replication.
- Enzymes play a crucial role in this process.
During DNA replication, what is the primary function of helicase?
During DNA replication, what is the primary function of helicase?
- To disrupt the hydrogen bonds between complementary bases. (correct)
- To catalyze the formation of complementary base pairs.
- To supply the energy needed for replication.
- To break the bonds between deoxyribose sugars and phosphate groups.
Which method is employed to separate DNA fragments based on their size during DNA profiling?
Which method is employed to separate DNA fragments based on their size during DNA profiling?
- Gel electrophoresis (correct)
- Titration
- Chromatography
- Autoradiography
What is the crucial role of DNA polymerase during replication?
What is the crucial role of DNA polymerase during replication?
Why is the presence of multiple replication origins advantageous in eukaryotic DNA replication?
Why is the presence of multiple replication origins advantageous in eukaryotic DNA replication?
What prevents the single strands of DNA from re-annealing during replication?
What prevents the single strands of DNA from re-annealing during replication?
What is the function of RNA primers during DNA replication?
What is the function of RNA primers during DNA replication?
How does DNA ligase contribute to the process of DNA replication?
How does DNA ligase contribute to the process of DNA replication?
What is the role of topoisomerase in DNA replication?
What is the role of topoisomerase in DNA replication?
Flashcards
DNA Replication
DNA Replication
The process where DNA makes two identical copies of itself.
Helicase
Helicase
An enzyme that unwinds the DNA double helix by breaking hydrogen bonds.
Semi-conservative Replication
Semi-conservative Replication
The principle where each new DNA molecule consists of one original and one new strand.
Gel Electrophoresis
Gel Electrophoresis
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Polymerase Chain Reaction (PCR)
Polymerase Chain Reaction (PCR)
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Role of Enzymes in DNA Replication
Role of Enzymes in DNA Replication
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Study Notes
- These notes pertain to IB Style Test - SL/HL Topic D1.1 DNA replication
- Theme D: Continuity and change
- Level of Organisation: Molecules
Multiple Choice Questions
- Adenine forms a complementary base pair with thymine, not cytosine
- Helicase breaks the hydrogen bonds between the bases
- DNA replication achieves two exact copies because base pairing is complementary and replication is semi-conservative
- Gel electrophoresis is the process used to separate DNA fragments in profiling
Structured Answer Questions
- The polymerase chain reaction and gel electrophoresis can identify a suspect from DNA left at a crime scene
- PCR amplifies DNA so there is enough to analyze
- Enzymes in the polymerase chain reaction catalyze the synthesis of new DNA strands
- Agarose gel in electrophoresis separates DNA fragments based on size
- An electric current in electrophoresis moves the negatively charged DNA fragments through the gel
- DNA samples from both the crime scene and the suspect are needed for comparison
- Observations must be made on many DNA fragments to create a unique profile
Extended Response Question
- Enzymes play a crucial role in DNA replication
Additional Higher Level Questions
- DNA replication is continuous on the leading strand, but discontinuous on the lagging strand
- DNA proofreading ensures that accurate copies of DNA are produced in DNA replication
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