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Questions and Answers
TK tissue culture plants grew faster in liquid media compared to solid media.
True
Traditional sterile tissue cultured plants grow rapidly on semi-solid medium in autoclavable vessels.
False
Axenic plant hydroponic culture has been limited to cell culture or callus grown on solid medium.
False
Taraxacum kok-saghyz is a root propagable species that can regenerate into whole plants without the addition of exogenous hormones.
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Plants grown in soil or soilless solid medium have roots that are easy to sterilize in advance of plant transformation experiments.
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Axenic tissue culture plants grown on semi-solid media produce large amounts of biomass compared to plants grown in solution-based media.
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Expanded clay pebbles provide excellent anchorage for growing roots while also allowing easy harvest for subsequent subculturing or tissue analysis.
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The hydroponically-grown plants showed 10 times larger growth than those on semi-solid media.
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The sucrose concentrations used in the experiment had very little effect on seedling growth on semi-solid media.
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The root biomass in the liquid system increased by up to 10 times compared to semisolid culture.
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Study Notes
Improved Axenic Hydroponic Method for Plant Transformation Studies
- Taraxacum kok-saghyz (TK) is a root propagable plant that produces high-quality natural rubber in its roots.
- TK requires modern molecular methods for domestication due to being wild-collected.
- A rapid plant transformation method using Agrobacterium rhizogenes root transformation was developed for TK.
- Sufficient root production for experimentation is a common hurdle in stable and transient root transformation methods.
- Traditional sterile tissue cultured plants grow slowly on semi-solid medium in autoclavable vessels.
- Hydroponic culture has low adoption due to the growth of unwanted micro-organisms in the nutrient-rich medium.
- Axenic plant hydroponic culture has been limited to cell culture or callus grown in suspension.
- Whole plant cultures must be oxygenated, but delicate root structures may be damaged by a shaker table.
- An easily implementable axenic culturing system for small-scale rapid root production is described.
- In a preliminary experiment, TK tissue culture plants grew significantly faster in liquid media compared to solid media.
- Addition of sucrose from 0.25 to 2% correlated with an increase in biomass accumulation in plants grown in liquid media.
- The hydroponic apparatus consists of autoclavable Magenta vessels with expanded clay balls and aeration hose fitted with a 20 μm filter.
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Description
Test your knowledge of an improved axenic hydroponic method for plant transformation studies with this quiz. Explore the development of a rapid plant transformation method using Agrobacterium rhizogenes root transformation for Taraxacum kok-saghyz (TK) and the challenges in achieving sufficient root production for experimentation. Delve into the benefits of hydroponic culture and the implementation of an axenic culturing system for small-scale rapid root production.
