Podcast
Questions and Answers
TK tissue culture plants grew faster in liquid media compared to solid media.
TK tissue culture plants grew faster in liquid media compared to solid media.
True
Traditional sterile tissue cultured plants grow rapidly on semi-solid medium in autoclavable vessels.
Traditional sterile tissue cultured plants grow rapidly on semi-solid medium in autoclavable vessels.
False
Axenic plant hydroponic culture has been limited to cell culture or callus grown on solid medium.
Axenic plant hydroponic culture has been limited to cell culture or callus grown on solid medium.
False
Taraxacum kok-saghyz is a root propagable species that can regenerate into whole plants without the addition of exogenous hormones.
Taraxacum kok-saghyz is a root propagable species that can regenerate into whole plants without the addition of exogenous hormones.
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Plants grown in soil or soilless solid medium have roots that are easy to sterilize in advance of plant transformation experiments.
Plants grown in soil or soilless solid medium have roots that are easy to sterilize in advance of plant transformation experiments.
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Axenic tissue culture plants grown on semi-solid media produce large amounts of biomass compared to plants grown in solution-based media.
Axenic tissue culture plants grown on semi-solid media produce large amounts of biomass compared to plants grown in solution-based media.
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Expanded clay pebbles provide excellent anchorage for growing roots while also allowing easy harvest for subsequent subculturing or tissue analysis.
Expanded clay pebbles provide excellent anchorage for growing roots while also allowing easy harvest for subsequent subculturing or tissue analysis.
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The hydroponically-grown plants showed 10 times larger growth than those on semi-solid media.
The hydroponically-grown plants showed 10 times larger growth than those on semi-solid media.
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The sucrose concentrations used in the experiment had very little effect on seedling growth on semi-solid media.
The sucrose concentrations used in the experiment had very little effect on seedling growth on semi-solid media.
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The root biomass in the liquid system increased by up to 10 times compared to semisolid culture.
The root biomass in the liquid system increased by up to 10 times compared to semisolid culture.
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Study Notes
Improved Axenic Hydroponic Method for Plant Transformation Studies
- Taraxacum kok-saghyz (TK) is a root propagable plant that produces high-quality natural rubber in its roots.
- TK requires modern molecular methods for domestication due to being wild-collected.
- A rapid plant transformation method using Agrobacterium rhizogenes root transformation was developed for TK.
- Sufficient root production for experimentation is a common hurdle in stable and transient root transformation methods.
- Traditional sterile tissue cultured plants grow slowly on semi-solid medium in autoclavable vessels.
- Hydroponic culture has low adoption due to the growth of unwanted micro-organisms in the nutrient-rich medium.
- Axenic plant hydroponic culture has been limited to cell culture or callus grown in suspension.
- Whole plant cultures must be oxygenated, but delicate root structures may be damaged by a shaker table.
- An easily implementable axenic culturing system for small-scale rapid root production is described.
- In a preliminary experiment, TK tissue culture plants grew significantly faster in liquid media compared to solid media.
- Addition of sucrose from 0.25 to 2% correlated with an increase in biomass accumulation in plants grown in liquid media.
- The hydroponic apparatus consists of autoclavable Magenta vessels with expanded clay balls and aeration hose fitted with a 20 μm filter.
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Description
Test your knowledge of an improved axenic hydroponic method for plant transformation studies with this quiz. Explore the development of a rapid plant transformation method using Agrobacterium rhizogenes root transformation for Taraxacum kok-saghyz (TK) and the challenges in achieving sufficient root production for experimentation. Delve into the benefits of hydroponic culture and the implementation of an axenic culturing system for small-scale rapid root production.
